1887

Abstract

Two monoclonal antibodies (MAbs) were obtained from BALB/c mice immunized with cowpox virus (CPV)-infected cells cultured in the presence of cytosine-l---arabinofuranosyl-HCl (Ara C). In the immunofluorescence test, the specific antigen reacting with these MAbs was restricted almost entirely to the nucleus in CPV-infected cells in the presence of Ara C at any time. On the other hand, in CPV-infected cells in the absence of Ara C, the antigen was first detectable within the nucleus 2 h after infection and migrated to the cytoplasm as the infection proceeded. On immuno- blotting, only one component with an of 27K was detected 2 to 24 h after infection of cells with CPV in either the presence or absence of Ara C. The antigen was also detected in vaccinia virus-infected cells, but not in any mock-infected cells nor in any virions purified from infected cells. These results suggest that the antigen reacting with the MAbs is virus-specific and a non-structural early antigen.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-235
1990-01-01
2024-11-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010235.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-235&mimeType=html&fmt=ahah

References

  1. Archard L. C. 1983; Synthesis of full-length virus genomic DNA by nuclei of vaccinia-infected HeLa cells. Journal of General Virology 64:2561–2575
    [Google Scholar]
  2. Baroudy B. M., Moss B. 1980; Purification and characterization of a DNA-dependent RNA polymerase from vaccinia virions. Journal of Biological Chemistry 255:4372–4380
    [Google Scholar]
  3. Becker Y., Joklik W. K. 1964; Messenger RNA in cells infected with vaccinia virus. Proceedings of the National Academy of Sciences, U.S.A 51:577–585
    [Google Scholar]
  4. Berkowitz D. M., Kakefuda T., Sporn M. D. 1969; A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei. Journal of Cell Biology 42:851–855
    [Google Scholar]
  5. Bloom D. C., Massung R., Savage L., Morrison D. K., Moyer R. W. 1989; Recruitment to the cytoplasm of a cellular lamin-like protein from the nucleus during a poxvirus infection. Virology 169:115–126
    [Google Scholar]
  6. Bolden A., Pedrali-Noy G., Weissbach A. 1979; Vaccinia virus infection of HeLa cells. II. Disparity between cytoplasmic and nuclear viral-specific RNA. Virology 94:138–145
    [Google Scholar]
  7. Cairns J. 1960; The initiation of vaccinia infection. Virology 11:603–623
    [Google Scholar]
  8. Dales S., Pogo B. G. T. 1981; Biology of poxviruses. In Virology Monographs 18 pp. 1–109 Kingsbury D. W., Zur Hausen H. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  9. Gafford L. G., Randall C. C. 1976; Virus-specific RNA and DNA in nuclei of cells infected with fowlpox virus. Virology 69:1–14
    [Google Scholar]
  10. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  11. Kitamoto N., Goto E., Tanimoto S., Tanaka T., Miyamoto H., Wakamiya N., Ikijta K., Ueda S., Kato S. 1984; Crossreactivity between cowpox and vaccinia viruses with monoclonal antibodies. Archives of Virology 82:129–136
    [Google Scholar]
  12. Kitamoto N., Tanimoto S., Hiroi K., Tanaka T., Miyamoto H., Wakamiya N., Ikuta K., Ueda S., Kato S. 1986; Polypeptide analysis with monoclonal antibodies of A type inclusion bodies induced by cowpox virus. Archives of Virology 89:15–28
    [Google Scholar]
  13. Kitamoto N., Tanimoto S., Hiroi K., Ozaki M., Miyamoto H., Wakamiya N., Ikuta K., Ueda S., Kato S. 1987; Monoclonal antibodies to cowpox virus: polypeptide analysis of several major antigens. Journal of General Virology 68:239–246
    [Google Scholar]
  14. Lacolla P., Weissbach A. 1975; Vaccinia virus infection of HeLa cells. I. Synthesis of vaccinia DNA in host cell nuclei. Journal of Virology 15:305–315
    [Google Scholar]
  15. Mcauslan B. R. 1969; The biochemistry of poxvirus replication. In The Biochemistry of Viruses pp. 361–413 Levy H. B. Edited by New York: Marcel Dekker;
    [Google Scholar]
  16. Miyamoto H., Kato S. 1968; Immune haemadsorption by cells infected with poxviruses. Biken Journal 11:343–353
    [Google Scholar]
  17. Morrison D. K., Moyer R. W. 1986; Detection of a subunit of cellular Pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions. Cell 44:587–596
    [Google Scholar]
  18. Morrison D. K., Carter J. K., Moyer R. W. 1985; Isolation and characterization of monoclonal antibodies directed against two subunits of rabbit poxvirus-associated, DNA-directed RNA poly-merase. Journal of Virology 55:670–680
    [Google Scholar]
  19. Moss B. 1974; Reproduction of poxviruses. In Comprehensive Virology 3 pp. 405–474 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  20. Moyer R. W. 1987; The role of the host cell nucleus in vaccinia virus morphogenesis. Virus Research 8:173–191
    [Google Scholar]
  21. Nevins J. R., Joklik W. K. 1977; Isolation and partial characterization of the poly(A) polymerases from HeLa cells infected with vaccinia virus. Journal of Biological Chemistry 252:6939–6947
    [Google Scholar]
  22. Prescott D. M., Kates J., Kirkpatrick J. B. 1971; Replication of vaccinia virus DNA in enucleated L-cells. Journal of Molecular Biology 59:505–508
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  24. Ueda Y., Ito M., Tagaya I. 1969; A specific surface antigen induced by poxvirus. Virology 38:180–182
    [Google Scholar]
  25. Wilton S., Dales S. 1986; Influence of RNA polymerase II upon vaccinia virus-related translation examined by means of alpha-amanitin. Virus Research 5:323–341
    [Google Scholar]
  26. Wilton S., Dales S. 1989; Relationship between RNA polymerase II and efficiency of vaccina virus replication. Journal of Virology 63:1540–1548
    [Google Scholar]
  27. Wing D., Weissbach A. 1984; Vaccinia virus RNA polymerase associated with nuclei of infected HeLa cells. Journal of Virology 49:26–34
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-1-235
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-235
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error