1887

Abstract

Synthetic peptides representing 14 regions of the bovine enterovirus structural proteins were used to raise antibodies in mice. The peptides were predicted using amino acid sequence alignments with the position of antigenic sites on other picornaviruses. Five of the anti-peptide antibodies reacted with the virus in an immunoprecipitation test. Furthermore, each of these anti-peptide antibodies neutralized virus infectivity; those directed against peptides of VP2 and VP3 neutralized to a greater extent than those directed against peptides of VP1. The positions of these epitopes in the viral structural proteins are discussed in relation to corresponding positions in other picornaviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-231
1990-01-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010231.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-231&mimeType=html&fmt=ahah

References

  1. Acharya R., Fry E., Stuart D., Fox G., Rowlands D., Brown F. 1989; The three dimensional structure of foot-and-mouth disease virus at 2·9 Å resolution. Nature; London: 337704–716
    [Google Scholar]
  2. Bittle J. L., Houghten R. A., Alexander H., Shannock T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease virus by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature; London: 29830–33
    [Google Scholar]
  3. Boege V., Kobasa D., Onodera S., Palmenberg A., Scraba D. 1989; Characterization of Mengovirus neutralization epitopes. Abstract. Sixth Meeting of the European Study Group on the Molecular Biology of Picornaviruses Bruges, Belgium: 10th to 16th September
    [Google Scholar]
  4. Earle J. A. P., Skuce R. A., Fleming C. S., Hoey E. M., Martin S. J. 1988; Complete nucleotide sequence of a bovine enterovirus. Journal of General Virology 69:253–263
    [Google Scholar]
  5. Emini E. A., Jameson B. A., Wimmer E. 1983; Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature; London: 304699–703
    [Google Scholar]
  6. Evans D. M. A., Minor P. D., Schild G. C., Almond J. W. 1983; Critical role of an eight amino acid sequence of VP1 in the neutralization of poliovirus type 3. Nature; London: 304459–462
    [Google Scholar]
  7. Ferguson M., Evans D. M. A., Magrath E. I., Minor P. D., Almond J. W., Schild G. C. 1985; Induction by synthetic peptides of broadly reactive, type specific neutralizing antibody to poliovirus type 3. Virology 143:505–515
    [Google Scholar]
  8. Francis M. J., Hastings G. Z., Sangar D. V., Clark R. P., Syred A., Clarke B. E., Rowlands D. J., Brown F. 1987; A synthetic peptide which elicits neutralizing antibody against human rhinovirus type 2. Journal of General Virology 68:2687–2691
    [Google Scholar]
  9. Hoey E. M., Martin S. J. 1974; A possible precursor containing RNA of a bovine enterovirus: the provirion II. Journal of General Virology 24:515–524
    [Google Scholar]
  10. Hogle J. M., Chow M., Filman D. J. 1985; Three dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  11. Houghten R. A. 1985; General method for the rapid synthesis of large numbers of peptides: specificity of an antigen-antibody interaction at the level of individual amino acids. Proceedings of the National Academy of Sciences, U.S.A 82:5131–5135
    [Google Scholar]
  12. Luo M., Vriend G., Kramer G., Minor I., Arnold E., Rossmann M. G., Boege V., Scraba D. G., Duke G. M., Palmenberg A. C. 1987; The atomic structure of Mengo virus at 3·0 Å resolution. Science 235:182–191
    [Google Scholar]
  13. Merrifield R. B. 1963; Solid phase peptide synthesis.I. The synthesis of a tetrapeptide. Journal of the American Chemical Society 85:2149–2154
    [Google Scholar]
  14. Minor P. D., Schild G. C., Bootman J., Evans D. M. A., Ferguson M., Reeve P., Spitz M., Stanway G., Cannon A. J., Hauptmann R., Clarke B. L. D., Mountford R. C., Almond J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature; London: 301674–679
    [Google Scholar]
  15. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  16. Palmenberg A. 1989; Sequence comparisons of the PI regions of picornaviruses. Proceedings ICN-VCI International Conference in Virology Newport Beach, California, U.S.A: Jan. 14 to 15 in press
    [Google Scholar]
  17. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picornaviruses. Nature; London: 317145–153
    [Google Scholar]
  18. Sherry B., Rueckert R. R. 1985; Evidence for at least two dominant neutralization antigens on human rhinovirus. Journal of Virology 53:137–143
    [Google Scholar]
  19. Sherry B., Mosser A. G., Colonno R. J., Rueckert R. R. 1986; Use of monoclonal antibodies to identify four neutralization immunogens on a common cold picomavirus, human rhinovirus 14. Journal of Virology 57:246–257
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-231
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error