1887

Abstract

Infection of cowpea cells with cowpea mosaic virus (CPMV) is accompanied by the appearance of tubular structures containing virus-like particles which protrude from or penetrate the cell wall. Immunogold labelling of sections of infected cells using antisera against a CPMV M RNA translation products, and Protein A-gold, showed that the 58K and/or 48K tentative transport proteins of CPMV were located in or on these tubular structures. Furthermore, these proteins were detected in small electron-dense areas near the tail-end of the tubules. The possible function of these structures in virus movement from cell to cell is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-219
1990-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010219.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-219&mimeType=html&fmt=ahah

References

  1. Atabekov J. G., Dorokhov Y. L. 1984; Plant virus-specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
    [Google Scholar]
  2. Dawson W. O., Schlegel D. E. 1976; Synchronization of cowpea chlorotic mottle virus replication in cowpea leaves. Intervirology 7:284–291
    [Google Scholar]
  3. Dorokhov Y. L., Miroshnichenko N. A., Alexandrova N. M., Atabekov J. G. 1981; Development of systemic TMV infection in upper noninoculated tobacco leaves after differential temperature treatment. Virology 108:507–509
    [Google Scholar]
  4. Eggen R., Van Kammen A. 1988; RNA replication in comoviruses. In RNA Genetics 1 pp. 49–70 Domingo E., Holland J. J., Ahlquist P. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  5. Franssen H., Goldbach R., Broekhuysen M., Moerman M., Van Kammen A. 1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  6. Goldbach R., Van Kammen A. 1985; Structure, replication and expression of the bipartite genome of cowpea mosaic virus. In Molecular Plant Virology 2 pp. 83–120 Davies J. W. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  7. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature; London: 286297–300
    [Google Scholar]
  8. Jones A. T., Kinninmonth A. M., Roberts I. M. 1973; Ultrastructural changes in differentiated leaf cells infected with cherry leaf roll virus. Journal of General Virology 18:61–64
    [Google Scholar]
  9. Kim K. S., Fulton J. P. 1971; Tubules with virus-like particles in leaf cells infected with bean pod mottle virus. Virology 43:329–337
    [Google Scholar]
  10. Kim K. S., Fulton J. P. 1973; Plant virus-induced cell wall overgrowth and associated membrane elaboration. Journal of Ultrastructure Research 45:328–342
    [Google Scholar]
  11. Malyshenko S. I., Lapchic L. G., Kondakova O. A., Kuznetzova L. L., Taliansky M. E., Atabekov J. G. 1988; Red clover mottlecomovirus B-RNA spreads between cells in tobamovirus- infected tissues. Journal of General Virology 69:407–412
    [Google Scholar]
  12. Martelli G. P. 1980; Ultrastructural aspects of possible defence reactions in virus-infected plant cells. Microbiologica 3:369–391
    [Google Scholar]
  13. Rezelman G., Franssen H. J., Goldbach R. W., Ie T. S., Van Kammen A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of General Virology 60:335–342
    [Google Scholar]
  14. Rezelman G., Van Kammen A., Wellink J. 1989; Expression of cowpea mosaic virus M RNA in cowpea protoplasts. Journal of General Virology 70:3043–3050
    [Google Scholar]
  15. Roberts I. M., Harrison B. D. 1970; Inclusion bodies and tubular structures in Chenopodium amaranticolor plants infected with strawberry latent ringspot virus. Journal of General Virology 7:47–54
    [Google Scholar]
  16. Takamatsu N., Ishikawa M., Meshi T., Okada Y. 1987; Expression of bacterial chloramphenicol acetyl-transferase gene in tobacco plants mediated by TMV-RNA. EMBO Journal 6:307–311
    [Google Scholar]
  17. Van Der Scheer C., Groenewegen J. 1971; Structure in cells of Vigna unguiculata infected with cowpea mosaic virus. Virology 46:493–497
    [Google Scholar]
  18. Van Lent J. W. M., Verduin B. J. M. 1986; Detection of viral protein and particles in thin sections of infected plant tissue using immunogoldlabelling. In Developments in Applied Biology. I. Developments and Applications in Virus Testing pp. 193–211 Jones R. A. C., Torrance L. Edited by Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  19. Van Lent J. W. M., Verduin B. J. M. 1987; Detection of viral antigen in semi-thin sections of plant tissue by immunogold-silver staining and light microscopy. Netherlands Journal of Plant Pathology 93:261–272
    [Google Scholar]
  20. Vos P., Verver J., Van Wezenbeek P., Van Kammen A., Goldbach R. 1984; Study of the genetic organization of a plant viral RNA genome by in vitro expression of a full-length DNA copy. EMBO Journal 3:3049–3053
    [Google Scholar]
  21. Vos P., Verver J., Jaegle M., Wellink J., Van Kammen A., Goldbach R. 1988; Two viral proteins involved in the proteolytic processing of the cowpea mosaic virus polyproteins. Nucleic Acids Research 16:1967–1985
    [Google Scholar]
  22. Wellink J., Van Kammen A. 1989; Cell-to-cell transport of cowpea mosaic virus requires both the 58K/48K proteins and the capsid proteins. Journal of General Virology 70:2279–2286
    [Google Scholar]
  23. Wellink J., Jaegle M., Prinz H., Van Kammen A., Goldbach R. 1987; Expression of the middle component RNA of cowpea mosaic virus in vivo . Journal of General Virology 68:2577–2585
    [Google Scholar]
  24. Wellink J., Van Lent J., Goldbach R. 1988; Detection of viral proteins in cytopathic structures in cowpea protoplasts infected with cowpea mosaic virus. Journal of General Virology 69:751–755
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-219
Loading
/content/journal/jgv/10.1099/0022-1317-71-1-219
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error