1887

Abstract

The major immediate early enhancer of human cytomegalovirus (HCMV) is known to exert a strong constitutive transcription stimulation in a broad spectrum of cells. This basal activity can be augmented considerably by elevated levels of intracellular cAMP in a cell type-specific manner. Cyclic AMP induction was observed in several lymphoid cell lines and in HeLa cells. One of the functionally important enhancer sequence modules, the 19 bp repeat element, mediates this effect as a cAMP-responsive element (CRE). It acts more efficiently than the corresponding sequence from the human chorionic gonadotropin gene. It is suggested that protein kinase C is involved in the pathway which leads to the activation of CRE- containing genes in lymphoid cells. Gel retardation assays indicated that similar, but not identical complexes are formed between nuclear protein extracts and the CREs of HCMV and the gonadotropin gene.

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1990-01-01
2022-11-27
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References

  1. Akrigg A., Wilkinson G. W. G., Oram J. D. 1985; The structure of the major immediate early gene of human cytomegalovirus AD 169. Virus Research 2:107–121
    [Google Scholar]
  2. Angel P., Imagawa M., Chiu R., Stein B., Imbra R. J., Rahmsdorf H. J., Jonat C., Herrlich P., Karin M. 1987; Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor. Cell 49:729–739
    [Google Scholar]
  3. Biocca S., Cattaneo A., Calissano P. 1983; A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12). EMBO Journal 2:643–648
    [Google Scholar]
  4. Boshart M., Weber F., Jahn G., Dorsch-Häsler K., Flecken-Stein B., Schaffner W. 1985; A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell 41:521–530
    [Google Scholar]
  5. Cambier J. C., Newell M. K., Justement L. B., Mcguire J. C., Leach K. L., Chen Z. Z. 1987; Ia binding ligands and cAMP stimulate nuclear translocation of PKC in B lymphocytes. Nature; London: 327629–632
    [Google Scholar]
  6. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. Cortes P., Buckbinder L., Leza M. A., Rak N., Hearing P., Merino A., Reinberg D. 1988; EivF, a factor required for transcription of the adenovirus EIV promoter, binds to an element involved in ElA-dependent activation and cAMP induction. Genes and Development 2:975–990
    [Google Scholar]
  8. Defranco D., Yamamoto K. R. 1986; Two different factors act separately or together to specify functionally distinct activities at a single transcriptional enhancer. Molecular and Cellular Biology 6:993–1001
    [Google Scholar]
  9. Delegeane A. M., Ferland L. H., Mellon P. L. 1987; Tissue- specific enhancer of the human glycoprotein hormone alpha-subunit gene: dependence on cyclic AMP-inducible elements. Molecular and Cellular Biology 7:3994–4002
    [Google Scholar]
  10. Deutsch P. J., Hoeffler J. P., Jameson J. L., Lin J. C., Habener J. F. 1988a; Structural determinants for transcriptional activation by cAMP-responsive DNA elements. Journal of Biological Chemistry 263:18466–18472
    [Google Scholar]
  11. Deutsch P. J., Hoeffler J. P., Jameson J. L., Habener J. F. 1988b; Cyclic AMP and phorbol ester-stimulated transcription mediated by similar DNA elements that bind distinct proteins. Proceedings of the National Academy of Sciences U.S.A.: 857922–7926
    [Google Scholar]
  12. Döffinger R., Pawlita M., Sczakiel G. 1988; Electrotransfection of human lymphoid and myeloid cell lines. Nucleic Acids Research 16:11840
    [Google Scholar]
  13. Fickenscher H., Stamminger T., Rüger R., Fleckenstein B. 1989; The role of a repetitive palindromic sequence element in the human cytomegalovirus major immediate early enhancer. Journal of General Virology 70:107–123
    [Google Scholar]
  14. Foecking M. K., Hofstetter H. 1986; Powerful and versatile enhancer-promoter unit for mammalian expression vectors. Gene 45:101–105
    [Google Scholar]
  15. Ghazal P., Lubon H., Fleckenstein B., Hennighausen L. 1987; Binding of transcription factors and creation of a large nucleoprotein complex on the human cytomegalovirus enhancer. Proceedings of the National Academy of Sciences U.S.A.: 843658–3662
    [Google Scholar]
  16. Ghazal P., Lubon H., Hennighausen L. 1988; Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro . Molecular and Cellular Biology 8:1809–1811
    [Google Scholar]
  17. Gonzalez G. A., Yamamoto K. R., Fischer W. H., Karr D., Menzel P., Biggs W. III Vale W. W., Montminy M. R. 1989; A cluster of phosphorylation sites on the cyclic AMP- regulated nuclear factor CREB predicted by its sequence. Nature; London: 337749–752
    [Google Scholar]
  18. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltrans- ferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  19. Hai T., Liu F., Allegretto E. A., Karin M., Green M. R. 1988; A family of immunologically related transcription factors that includes multiple forms of ATF and AP-1. Genes and Development 2:1216–1226
    [Google Scholar]
  20. Hardy S., Shenk T. 1988; Adenoviral control regions activated by E1A and the cAMP response element bind the same factor. Proceedings of the National Academy of Sciences U.S.A.: 854171–4175
    [Google Scholar]
  21. Hatzopoulos A. K., Schlokat U., Gruss P. 1988; Enhancers and other cis-acting regulatory sequences. In Transcription and Splicing pp 43–96 Hames B. D., Glover D. M. Edited by Oxford: IRL Press;
    [Google Scholar]
  22. Hunninghake G. W., Monick M. M., Liu B., Stinski M. F. 1989; The promoter-regulatory region of the major immediate-early gene of human cytomegalovirus responds to T-lymphocyte stimu- lation and contains functional cyclic AMP-response elements. Journal of Virology 63:3026–3033
    [Google Scholar]
  23. Hurst H. C., Jones N. C. 1987; Identification of factors that interact with the El A-inducible adenovirus E3 promoter. Genes and Development 1:1132–1146
    [Google Scholar]
  24. Hyman S. E., Comb M., Lin Y. S., Pearlbert J., Green M. R., Goodman H. M. 1988; A common trans-acting factor is involved in transcriptional regulation of neurotransmitter genes by cyclic AMP. Molecular and Cellular Biology 8:4225–4233
    [Google Scholar]
  25. Jahn G., Knust E., Schmolla H., Sarre T., Nelson J. A., Mcdougall J. K., Fleckenstein B. 1984; Predominant immediate-early transcripts of human cytomegalovirus AD 169. Journal of Virology 49:363–370
    [Google Scholar]
  26. Klein G., Lindahl T., Leibold W., Menezes J., Nilsson K., Sundström C. 1974; Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proceedings of the National Academy of Sciences U.S.A: 713283–3286
    [Google Scholar]
  27. Lee W., Mitchell P., Tjian R. 1987; Purified transcription factor AP-1 interacts with TP A-inducible enhancer elements. Cell 49:741–752
    [Google Scholar]
  28. Lengyel P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251
    [Google Scholar]
  29. Lin Y.-S., Green M. R. 1988; Interaction of a common cellular transcription factor, ATF, with regulatory elements in both El a- and cyclic AMP-inducible promoters. Proceedings of the National Academy of Sciences U.S.A.: 853396–3400
    [Google Scholar]
  30. Lubon H., Ghazal P., Hennighausen L., Reynolds-Kohler C., Lockshin C., Nelson J. A. 1989; Cell-specific activity of the modulator region in the human cytomegalovirus major immediate early gene. Molecular and Cellular Biology 9:1342–1345
    [Google Scholar]
  31. Mach M., Stamminger T., Jahn G. 1989; Human cytomegalovirus: recent aspects from molecular biology. Journal of General Virology 70:3117–3146
    [Google Scholar]
  32. Maekawa T., Sakura H., Kanei-Ishii C., Sudo T., Yoshimura T., Fujisawa J.-L, Ishii S. 1989; Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO Journal 8:2023–2028
    [Google Scholar]
  33. Miksicek R., Heber A., Schmid W., Danesch U., Posseckert G., Beato M., Schutz G. 1986; Glucocorticoid responsiveness of the transcriptional enhancer of Moloney murine sarcoma virus. Cell 46:283–290
    [Google Scholar]
  34. Minowada J., Ohnuma T., Moore G. E. 1972; Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. Journal of the National Cancer Institute 49:891–895
    [Google Scholar]
  35. Montminy M. R., Bilezikjian L. M. 1987; Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature; London: 328175–178
    [Google Scholar]
  36. Montminy M. R., Sevarino K. A., Wagner J. A., Mandel G., Goodman R. H. 1986; Identification of a cyclic AMP-responsive element within the rat somatostatin gene. Proceedings of the National Academy of Sciences U.S.A.: 836682–6686
    [Google Scholar]
  37. Mosthaf L., Pawlita M., Gruss P. 1985; A viral enhancer element specifically active in human haematopoietic cells. Nature; London: 315597–600
    [Google Scholar]
  38. Picard D. 1985; Viral and cellular transcription enhancers. Oxford Surveys on Eukaryotic Genes 2:24
    [Google Scholar]
  39. Queen C., Baltimore D. 1983; Immunoglobulin gene transcription is activated by downstream sequence elements. Cell 33:741–748
    [Google Scholar]
  40. Roesler W. J., Vandenbark G. R., Hanson R. W. 1988; Cyclic AMP and the induction of eukaryotic gene transcription. Journal of Biological Chemistry 263:9063–9066
    [Google Scholar]
  41. Schneider U., Schwenk J.-U., Bornkamm G. 1977; Characterization of EBV-genome negative ‘null’ and ‘T’ cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. International Journal of Cancer 19:621–626
    [Google Scholar]
  42. Schrier R. D., Nelson J. A., Oldstone M. B. A. 1985; Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science 230:1048–1051
    [Google Scholar]
  43. Seamon K. B., Padgett W., Daly J. W. 1981; Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proceedings of the National Academy of Sciences U.S.A.: 783363–3367
    [Google Scholar]
  44. Serfling E., Jasin M., Schaffner W. 1985; Enhancers and eukaryotic gene transcription. Trends in Genetics 1:224–230
    [Google Scholar]
  45. Silhavy T. J., Berman M. L., Enquist L. W. 1984 Experiments with Gene Fusions New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  46. Silver B. J., Bokar J. A., Virgin J. B., Vallen E. A., Milstedt A., Nilson J. H. 1987; Cyclic AMP regulation of the human glycoprotein hormone alpha-subunit gene is mediated by an 18-base- pair element. Proceedings of the National Academy of Sciences U.S.A.: 842198–2202
    [Google Scholar]
  47. Sinclair J. H. 1987; The human cytomegalovirus immediate early gene promoter is a strong promoter in cultured Drosophila melanogaster cells. Nucleic Acids Research 5:2392
    [Google Scholar]
  48. Tamaoki T., Nomoto H., Takahashi I., Kato Y., Morimoto M., Tomita F. 1986; Staurosporin, a potent inhibitor of phospholipid/ Ca++ dependent protein kinase. Biochemical and Biophysical Research Communications 135:397–402
    [Google Scholar]
  49. Thomsen D. R., Stenberg R. M., Goins W. F., Stinski M. F. 1984; Promoter-regulatory region of the major immediate early gene of human cytomegalovirus. Proceedings of the National Academy of Sciences U.S.A.: 81659–663
    [Google Scholar]
  50. Westin G., Gerster T., Müller M. M., Schaffner G., Schaffner W. 1987; OVEC, a versatile system to study transcription in mammalian cells and cell-free extracts. Nucleic Acids Research 15:6787–6798
    [Google Scholar]
  51. Wolf M., Baggiolini M. 1988; The protein kinase inhibitor staurosporin, like phorbol esters, induces the association of protein kinase C with membranes. Biochemical and Biophysical Research Communications 154:1273–1279
    [Google Scholar]
  52. Yamamoto K. R. 1985; Steroid receptor regulated transcription of specific genes and gene networks. Annual Review of Genetics 19:209
    [Google Scholar]
  53. Yamamoto K. R., Gonzalez G. A., Biggs W. H. III Montminy M. R. 1988; Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature; London: 334494–498
    [Google Scholar]
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