1887

Abstract

Summary

The nucleotide sequences of cDNA clones corresponding to 2569 nucleotides from the 3′ end of cymbidium ringspot tombusvirus (CyRSV) RNA were determined. This region contains three open reading frames giving rise to three predicted protein products, two of which had been identified in previous studies. The 3′ non-coding region is 351 nucleotides long. The amino acid sequence of CyRSV coat protein has striking similarities with that of tomato bushy stunt tombusvirus, particularly in the S domain. No homology was found between the protein encoded by the second largest open reading frame and the corresponding product of other plant viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-9-2533
1989-09-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/9/JV0700092533.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-9-2533&mimeType=html&fmt=ahah

References

  1. Bellon B. 1988; Apple Macintosh programs for nucleic and protein sequence analyses. Nucleic Acids Research 16:1837–1846
    [Google Scholar]
  2. Burgyan J., Russo M., Galutelli D. 1986; Translation of cymbidium ringspot virus RNA in cowpea protoplasts and rabbit reticulocyte lysates. Journal of General Virology 67:1149–1160
    [Google Scholar]
  3. Burgyan J., Grieco F., Russo M. 1989; A defective interfering RNA molecule in cymbidium ringspot virus infections. Journal of General Virology 70:235–239
    [Google Scholar]
  4. Carrington J. C., Morris T. J. 1986; High resolution mapping of carnation mottle virus-associated RNAs. Virology 150:196–206
    [Google Scholar]
  5. Chen E. J., Seeburg P. H. 1985; Supercoil sequencing: a fast simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  6. Done-Keller H., Maxam A. M., Gilbert W. 1977; Mapping adenines, guanines and pyrimidines in RNA. Nucleic Acids Research 4:2527–2538
    [Google Scholar]
  7. England T. E., Uhlenbeck O. C. 1978; 3′-Terminal labelling of RNA with T4 RNA ligase. Nature London: 275560–561
    [Google Scholar]
  8. Fickett J. W. 1982; Recognition of protein coding regions in DNA sequences. Nucleic Acids Research 10:5303–5318
    [Google Scholar]
  9. Gallitelli D., Hull R., Koenig R. 1985; Relationships among viruses in the tombusvirus group: nucleic acid hybridization studies. Journal of General Virology 66:1523–1531
    [Google Scholar]
  10. Gomez-Marquez J., Freire M., Segade F. 1987; A simple procedure for large-scale purification of plasmid DNA. Gene 54:255–259
    [Google Scholar]
  11. Hayes R. J., Brunt A. A., Buck K. W. 1988; Gene mapping and expression of tomato bushy stunt virus. Journal of General Virology 69:3047–3057
    [Google Scholar]
  12. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  13. Hillman B. I., Hearne P., Rochon D., Morris T. J. 1989; Organization of tomato bushy stunt virus genome: characterization of the coat protein gene and the 3′ terminus. Virology 169:42–50
    [Google Scholar]
  14. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmid DNA. Analytical Biochemistry 114:193–197
    [Google Scholar]
  15. Hopper P., Harrison S. C., Sauer R. T. 1984; Structure of tomato bushy stunt virus. V. Coat protein sequence determination and its structural implications. Journal of Molecular Biology 177:701–713
    [Google Scholar]
  16. Koenig R., Gibbs A. 1986; Serological relationships among tombusviruses. Journal of General Virology 67:75–82
    [Google Scholar]
  17. Kozak M. 1984; Point mutation close to AUG initiator codon affects the efficiency of translation of rat preproinsulin in vivo. Nature london: 308241–246
    [Google Scholar]
  18. Marck C. 1988; ‘DNA Strider’: a ‘C’ program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.. Nucleic Acids Research 16:1829–1836
    [Google Scholar]
  19. Messing I. 1983; New vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  20. Russo M., Burgyan J., Carrington J. C., Hillman B. I., Morris T. I. 1988; Complementary DNA cloning and characterization of cymbidium ringspot virus RNA. Journal of General Virology 69:401–406
    [Google Scholar]
  21. Saito T., Imai Y., Meshi T., Okada Y. 1988; Interviral homologies of the 30K proteins of tobamoviruses. Virology 167:653–656
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 74:5463–5467
    [Google Scholar]
  23. Wurst R. M., Vournakis J. N., Maxam A. M. 1978; Structure mapping of 5′-32P-labeled RNA with S1 nuclease. Biochemistry 17:4493–4499
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-9-2533
Loading
/content/journal/jgv/10.1099/0022-1317-70-9-2533
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error