Antigen-presenting Capacity of Epidermal Cells Infected with Vaccinia Virus Recombinants Containing the Herpes Simplex Virus Glycoprotein D, and Protective Immunity
We studied the association of herpes simplex type 1 (HSV-1) glycoprotein D (gD-1) expression in epidermal cells (EC) with virus-specific immunity and protection of mice from fatal HSV-2 challenge. Vaccinia virus recombinants containing gD-1 under the control of an early (VP176) or late (VP254) vaccinia virus promoter were used. Mature gD-1 protein was expressed in VP176-infected EC and they had accessory cell function for HSV-2-induced T cell proliferation of immune lymph node cells (LNC). It was not expressed in VP254-infected EC and they did not act as accessory cells. LNC from VP176- but not VP254-immunized mice proliferated in response to HSV antigen and only VP176-immunized mice had complete long-term protection from HSV-2 challenge.
BablanianR.1984; Poxvirus cytopathogenicity: effects on cellular macromolecular synthesis. In Comprehensive Virology19391–421Fraenkel-ConratH.,
WagnerR. R.
New York: Plenum Press;
BalachandranN.,
BacchetttS.,
RawlsW. E.1982; Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. Infection and Immunity 37:1132–1137
BertholetC.,
DrillienR.,
WittekR.1985; One hundred base pairs of 5’ flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription. Proceedings of the National Academy of SciencesU.S.A. 82:2096–2100
BullerR. M. L.,
MossB.1985; Genetic basis for vaccinia virus virulence. In Vaccinia Viruses as Vectors for Vaccine Antigens37–46QuinnanG. V.JR
Amsterdam: Elsevier;
CohenG. h.,
LongD.,
EkenbergR. J.1980; Synthesis and processing of glycoprotein gD and gC of herpes simplex virus type 1. Journal of Virology 36:429–439
CouparB. E. H.,
AndrewM. E.,
BothG. W.,
BoyleD. B.1986; Temporal regulation of influenza hemagglutinin expression in vaccinia virus recombinants and effects on the immune response. European Journal of Immunology 16:1479–1487
EisenbergR. J.,
Ponce de leonM.,
PereiraL.,
LongD.,
CohenG. H.1982; Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody. Journal of Virology 41:1099–1104
FriedmanR. M.,
BaronS.,
BucklerC. E.,
SteinmullerR. L.1962; The role of antibody, delayed hypersensitivity, and interferon production in recovery of guinea pigs from primary infection with vaccinia virus. Journal of Experimental Medicine 116:347–356
HayashiY.,
AurelianL.1986; Immunity to herpes simplex virus type 2: viral antigen presenting capacity of epidermal cells and its impairment by ultraviolet irradiation. Journal of Immunology 136:1087–1092
HowesE. L.,
TaylorW.,
MitchisonN. A.,
SimpsonE.1979; MHC matching shows that at least two T-cell subsets determine resistance to HSV. Nature London: 27767–68
KotwalG. J.,
MossB.1988; Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant. Virology 167:524–537
MartinS.,
RouseB. T.1987; The mechanism of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D: clearance of local infection. Journal of Immunology 138:3431–3437
NashA. A.,
JayasuriyaA.,
PhelanJ.,
CobboldS. P.,
waldmannH.,
ProsperoT.1987; Different roles for L3T4+ and Lyt 2+ T cell subsets in the control of an acute herpes simplex virus infection of the skin and nervous system. Journal of General Virology 68:825–833
NatukR. J.,
HolowczakJ. A.1985; Vaccinia virus proteins on the plasma membrane of infected cells. III. Infection of peritoneal macrophages. Virology 147:354–372
PanicaliD.,
DavisS. W.,
MercerS. R.,
PaolettiE.1981; Two major DNA variants present in serially propagated stocks of WR strain of vaccinia virus. Journal of Virology 37:1000–1010
WachsmanM.,
AurelianL.,
SmithC. C.,
LipinskasB. R.,
PerkusM. E.,
Paolettie.1987; Protection of guinea pigs from primary and recurrent herpes simplex virus (HSV) type 2 cutaneous disease with vaccinia virus recombinants expressing HSV glycoprotein D. Journal of Infectious Diseases 155:1188–1197
WachsmanM.,
AurelianL.,
HunterJ. C. R.,
PerkusM. E.,
PaolettiE.1988; Expression of herpes simplex virus glycoprotein D on antigen presenting cells infected with vaccinia recombinants and protective immunity. Bioscience Reports 8:323–334
Antigen-presenting Capacity of Epidermal Cells Infected with Vaccinia Virus Recombinants Containing the Herpes Simplex Virus Glycoprotein D, and Protective Immunity