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Synthetic oligopeptides, corresponding to an amino acid sequence encoded by a potential M r 9000 product’s open reading frame (ORF-4) at the 3′ terminus of the transmissible gastroenteritis virus genome, were used to generate rabbit antiserum. These antibodies produced immune complexes with an M r 14000 (14K) polypeptide in infected cells. The 14K product was shown by immune fluorescence to become associated with the cell nucleus, correlating with the onset of nuclear vacuolation, and suggesting a role in pathogenesis for the ORF-4 gene.
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