Acid Stability of Hepatitis A Virus Free

Abstract

Summary

The acid stability of unpurified and highly purified hepatitis A virus (HAV) was tested andcompared with that of poliovirus type 1, coxsackievirus types A9 and B1 and echovirus type 9. Only HAV had a high residual infectivity after 2 h of exposure to pH 1 at room temperature, remaining infectious for up to 5 h. At 38°C, pH 1, HAV remained infectious for 90 min. Highly purified HAV was found to be infectious for 8 h at pH 1 and room temperature. This indicates that the increased stability is not due to protection by cellular material attached to the virus,but is a virus-specific marker. Under the same conditions, at pH 1 and room temperature, unpurified and highly purified HAV antigens were traceable for 5 and 4 h respectively.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-9-2481
1989-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/9/JV0700092481.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-9-2481&mimeType=html&fmt=ahah

References

  1. Ansorge W. 1983; Fast visualization of protein bands by impregnation in potassium permanganate and silver nitrate. In Electrophoresis ’82235–242 Stathakos D. Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  2. Coulepis A. G., , Anderson B. N., Gust I. d. 1987; Hepatitis A. Advances in Virus Research 32:129–169
    [Google Scholar]
  3. Faber H. K., Dong L. 1946; Inactivation of poliomyelitis virus in relation to gastric and intestinal digestion. Proceedings of the Society for Experimental Biology and Medicine 63:575–578
    [Google Scholar]
  4. Flehmig B., Ranke M., Frank H., Gerth H. J. 1978; Application of a solid-phase radioimmunoassay and immune electron microscopy for hepatitis A in diagnosis and research. Medical Microbiology and Immunology 166:187–194
    [Google Scholar]
  5. Flehmig B., Vallbracht A., Wurster G. 1981; Hepatitis A virus in cell culture. III. Propagation of hepatitis A virus in human embryo kidney cells and human embryo fibroblast strains. Medical Microbiology and Immunology 170:83–89
    [Google Scholar]
  6. Heinricy U., Stierhof Y.-D., Pfisterer M., Flehmig B. 1987; Properties of a hepatitis A virus candidate vaccine strain. Journal of General Virology 68:2487–2493
    [Google Scholar]
  7. Kärber G. 1931; Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv für experimentelle Pathologie und Pharmakologie 162:480–483
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  9. Lemon S. M., Binn L. N. 1985; Incomplete neutralization of hepatitis A virus in vitro due to lipid-associated virions. Journal of General Virology 66:2501–2505
    [Google Scholar]
  10. Lonberg-holm K., Philipson L. 1981; Pathogenesis of picornaviruses. In Virus Receptors 2171–174 London & New York: Chapman and Hall;
    [Google Scholar]
  11. Loring H. S., Schwerdt C. P. 1944; Studies on purification of poliomyelitis virus. II. pH stability range of MVA strain. Proceedings of the Society for Experimental Biology and Medicine 57:173–175
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Newman J. F. E., Rowlands D. J., Brown F. 1973; A physico-chemical sub-grouping of the mammalian picornaviruses. Journal of General Virology 18:171–180
    [Google Scholar]
  14. Provost P. J., Hughes J. V., Miller W. J., Giesa P. A., Banker F. S., Emini E. A. 1986; An inactivated hepatitis A viral vaccine of cell culture origin. Journal of Medical Virology 19:23–31
    [Google Scholar]
  15. Pschyrembel W. 1969 Klinisches Wörterbuch728 Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  16. Robinson L. K. 1950; Effect of heat and of pH on strains of Coxsackie virus. Proceedings of the Society for Experimental Biology and Medicine 75:580–582
    [Google Scholar]
  17. Salo R. J., Cliver D. O. 1976; Effect of acid pH, salts, and temperature on the infectivity and physical integrity of enteroviruses. Archives of Virology 52:269–282
    [Google Scholar]
  18. Siegl G., Weitz M., Kronauer G. 1984; Stability of hepatitis A virus. Intervirology 22:218–226
    [Google Scholar]
  19. Vallbracht A., Hofmann L., Wurster K. G., Flehmig B. 1984; Persistent infection of human fibroblasts by hepatitis A virus. Journal of General Virology 65:609–615
    [Google Scholar]
  20. White D. O., Fenner F. J. 1986; Pathogenesis and pathology of viral infections (chapter 5), Picornaviruses and calciviruses (chapter 19). In Medical Virology119–145–451–478 Orlando: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-9-2481
Loading
/content/journal/jgv/10.1099/0022-1317-70-9-2481
Loading

Data & Media loading...

Most cited Most Cited RSS feed