1887

Abstract

Summary

A cassette vector has been constructed which allows the rapid and extensive modification of one of the neutralizing antigenic sites of the Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease sites flanking antigenic site 1 have been engineered into a full-length infectious Sabin 1 cDNA clone with minimal alteration to the coding sequence. This facilitates replacement of this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that this region is highly flexible in terms of the number and sequence of amino acids which can be accommodated.

Keyword(s): antigen chimaeras , poliovirus and vector
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-9-2475
1989-09-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/9/JV0700092475.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-9-2475&mimeType=html&fmt=ahah

References

  1. Burke K. L., Dunn G., Ferguson M., Minor P. D., Almond J. W. 1988; Antigen chimaeras of poliovirus as potential new vaccines. Nature, London 332:81–82
    [Google Scholar]
  2. Emini A. E., Bradford A. J., Wimmer E. 1983; Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature, London 304:699–703
    [Google Scholar]
  3. Ferguson M., Minor P. D., Magrath D. I., qui Y-H., spitz M., Schild G. C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  4. Ferguson M., Evans D. M. A., Magrath D. I., Minor P. D., Almond J. W., Schild G. C. 1985; Induction by synthetic peptides of broadly reactive, type-specific neutralizing antibody to poliovirus type 3. Virology 143:505–515
    [Google Scholar]
  5. Ferguson M., Reed S. E., Minor P. D. 1986; Reactivity of anti-peptide and anti-poliovirus type 3 monoclonal antibodies with synthetic peptides. Journal of General Virology 67:2527–2531
    [Google Scholar]
  6. Hogle J. M., Chow M., Filman D. J. 1985; The three-dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  7. Jenkins O., Booth J. D., Minor P. D., Almond J. W. 1987; The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the Picornaviridae. Journal of General Virology 68:1835–1848
    [Google Scholar]
  8. Kitamura N., Semler B., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecack R., Lee J. J., van der werf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
    [Google Scholar]
  9. Kramer W., Drutsa V., Jansen H., Kramer B., Pflugfelder M., Fritz H. 1984; The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Research 12:9441–9456
    [Google Scholar]
  10. Kuhn R. J., Tada H., Ypma-wong M. F., Dunn J. J., Semler B. L., Wimmer E. 1988; Construction of a ‘mutagenesis cartridge’ for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants. Proceedings of the National Academy of SciencesU.S.A 85:519–523
    [Google Scholar]
  11. Mccray J., Werner G. 1987; Different rhinovirus serotypes neutralized by antipeptide antibodies. Nature, London 329:736–738
    [Google Scholar]
  12. Martin A., Wychowski C., Couderc T., Crainic R., Hogle J. M., Girard M. 1988; Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO Journal 7:2839–2847
    [Google Scholar]
  13. Melnick J. L. 1980; Poliomyelitis vaccines: an appraisal after 25 years. Comprehensive Therapeutics 5:6–14
    [Google Scholar]
  14. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1292
    [Google Scholar]
  15. Murray M. G., Kuhn R. J., Arita M., Kawamura N., Nomoto A., Wimmer E. 1988; Poliovirus type 1/type 3 antigenic hybrid virus constructed in vitro elicits type 1 and type 3 neutralizing antibodies in rabbits and monkeys. Proceedings of the National Academy of SciencesU.S.A. 85:3202–3207
    [Google Scholar]
  16. Najarian R., Caput D., Gee W., Potter S., Renard A., Merrywheather J., van nest G., Dina D. 1985; Primary structure and gene organization of human hepatitis A virus. Proceedings of the National Academy of SciencesU.S.A 82:2627–2631
    [Google Scholar]
  17. Ogra P. L., Fishaut M., Gallagher M. R. 1980; Viral vaccination via the mucosal routes. Review of Infectious Diseases 2:352–369
    [Google Scholar]
  18. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  19. Pipkin P. A., Minor P. D., Hockley D., Schild G. C., Almond J. W. 1984; Monoclonal antibodies which block cellular receptors of poliovirus. Virus Research 1:203–212
    [Google Scholar]
  20. Purcell R. H. 1987; Status of hepatitis A vaccines. In Positive Strand RNA Viruses (UCLA Symposia on Molecular and Cellular Biology)565–574 Brinton M. R., Rueckert R. R.
    [Google Scholar]
  21. Rico-Hesse R., Pallansch M. A., Nottay B. K., Kew O. M. 1987; Geographic distribution of wild poliovirus type 1 genotypes. Virology 160:311–322
    [Google Scholar]
  22. Sabin A. B., Boulger L. R. 1973; History of Sabin attenuated poliovirus oral live vaccine strains. Journal of Biological Standards 1:115–118
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A 74:5463–5467
    [Google Scholar]
  24. Skern T., Sommergruber W., Blaas D., Gruendler p., Fraundorfer F., Pieler C., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  25. Stanway G., Hughes P., Mountford R., Minor P., Almond J. W. 1984; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
  26. Stanway G., Hughes P. J., Westrop G. D., Evans D. M. A., Dunn G., Minor P. D., Schild G. C., Almond J. W. 1986; Construction of poliovirus intertypic recombinants by use of cDNA. Journal of Virology 57:1187–1190
    [Google Scholar]
  27. Van der werf S., Wychowski C., Bruneau P., Blondel B., Crainic R., Horodniceanu F., Girard M. 1983; Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1. Proceedings of the National Academy of SciencesU.S.A. 80:5080–5084
    [Google Scholar]
  28. van der werf S., Bradley J., Wimmer E., Studier F. W., Dunn J. J. 1986; Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proceedings of the National Academy of SciencesU.S.A 83:2330–2334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-9-2475
Loading
/content/journal/jgv/10.1099/0022-1317-70-9-2475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error