1887

Abstract

Summary

The negative-stranded genomic RNA of lettuce necrotic yellows virus (LNYV) was isolated and estimated by denaturing agarose gel electrophoresis to consist of about 13000 nucleotides (nt). Hybridization of randomly labelled LNYV RNA to poly(A) RNA from infected plants revealed the presence of five distinct complementary RNA species (cRNAs) ranging in size from 900 to 6450 nt. Individual recombinant cDNA clones derived from LNYV RNA hybridized to four of these cRNA species. Each cRNA contained unique sequences which together represented about 95% of the viral genome. From their size range and sequence complexity, it is assumed that the cRNAs represent messenger RNAs encoding the five LNYV structural proteins. The mRNA for the nucleocapsid protein is cRNA 3 because a recombinant cDNA expression vector clone containing sequences which hybridize to cRNA 3 produced a polypeptide which was detected with a monoclonal antibody specific for the nucleocapsid protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-9-2299
1989-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/9/JV0700092299.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-9-2299&mimeType=html&fmt=ahah

References

  1. Banerjee A. K. 1987; The transcription complex of vesicular stomatitis virus. Cell 48:363–364
    [Google Scholar]
  2. Covey S. N., Lomonossoff G. P., Hull R. 1981; Characterization of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts. Nucleic Acids Research 9:6735–6747
    [Google Scholar]
  3. Dale R. M. K., Mcclure B. A., Houchins J. P. 1985; A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: applications to sequencing the corn mitochondrial 18S DNA. Plasmid 13:31–40
    [Google Scholar]
  4. D’Alessio J. M., Gerard G. F. 1988; Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase mH: the fate of information at the mRNA 5′ terminus and the effect of E. coli DNA ligase. Nucleic Acids Research 16:1999–2014
    [Google Scholar]
  5. Dietzgen R. G., Francki R. I. B. 1986; Nonspecific binding of immunoglobulins to coat proteins of certain plant viruses in immunoblots and indirect ELISA. Journal of Virological Methods 15:159–164
    [Google Scholar]
  6. Dietzgen R. G., Francki R. I. B. 1988; Analysis of lettuce necrotic yellows virus structural proteins with monoclonal antibodies and concanavalin A. Virology 166:486–494
    [Google Scholar]
  7. Francki R. I. B., Randles J.W. 1972; RNA-dependent RNA polymerase associated with particles of lettuce necrotic yellows virus. Virology 47:270–275
    [Google Scholar]
  8. Francki R. I. B., Randles J.W. 1973; Some properties of lettuce necrotic yellows virus RNA and its in vitro transcription by virion-associated transcriptase. Virology 54:359–368
    [Google Scholar]
  9. Francki R. I. B., Randles J. W., Dietzgen R. G. 1989; Lettuce necrotic yellows virus. CMI/AAB Descriptions of Plant Viruses
    [Google Scholar]
  10. Freeman G. J., Rose J. K., Clinton G. M., Huang A.S. 1977; RNA synthesis of vesicular stomatitis virus. VII. Complete separation of the mRNA’s of vesicular stomatitis virus by duplex formation. Journal of Virology 21:1094–1104
    [Google Scholar]
  11. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of SciencesU.S.A. 72:3961–3965
    [Google Scholar]
  12. Gustafson G. D., Milner J. J., Mcfarland J. E., Pedersen K., Larkins B. A., Jackson A. O. 1982; Investigation of the complexity of barley stripe mosaic virus RNAs with recombinant DNA clones. Virology 120:182–193
    [Google Scholar]
  13. Heaton L. A. 1986; Characterization and analysis of the genome of sonchus yellow net virus. Ph.D. thesis; Purdue University:
    [Google Scholar]
  14. Heaton L. A., Hillman B. L., Zuidema D., Jackson A. O. 1987a; Sonchus yellow net virus: a plant rhabdovirus with six genes. Abstracts of the 7th International Congress of Virology Edmonton, Canada:225 R33.7
    [Google Scholar]
  15. Heaton L. A., Zuidema D., Jackson A. O. 1987b; Structure of the M2 protein gene ofsonchus yellow net virus. Virology 161:234–241
    [Google Scholar]
  16. Helfman D. M., Hughes S. H. 1987; Use of antibodies to screen cDNA expression libraries prepared in plasmid vectors. Methods in Enzymology 152:451–457
    [Google Scholar]
  17. Hellebust H., Murby M., Abrahamsen L., Uhlen M., Enfors S. 1989; Different approaches to stabilize a recombinant fusion protein. Bio/Technology 7:165–168
    [Google Scholar]
  18. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  19. Hu N., Messing J. 1982; The making of strand-specific M13 probes. Gene 17:271–277
    [Google Scholar]
  20. Hunter B. G., Heaton L. A., Bracker C. E., Jackson A. O. 1986; Structural comparison of poa semilatent virus and barley stripe mosaic virus. Phytopathology 75:322–326
    [Google Scholar]
  21. Hurkman W. J., Tanaka, C K. 1986; Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiology 81:802–806
    [Google Scholar]
  22. Jackson A. O., Christie S. R. 1977; Purification and some physicochemical properties of sonchus yellow net virus. Virology 77:344–355
    [Google Scholar]
  23. Jackson A. O., Francki R. I. B., Zuidema D. 1987; Biology, structure and replication of plant rhabdoviruses. In The Rhabdoviruses427–508 Wagner R. R. New York:: Plenum Press;
    [Google Scholar]
  24. Jacobson A. 1987; Purification and fractionation of poly(A)+ RNA. Methods in Enzymology 152:254–261
    [Google Scholar]
  25. Kurath G., Leong J. C. 1985; Characterization of infectious hematopoietic necrosic virus mRNA species reveals a non-virion rhabdovirus protein. Journal of Virology 53:462–468
    [Google Scholar]
  26. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  27. McLean G. D., Wolanski B. S., Francki R. I. B. 1971; Serological analysis of lettuce necrotic yellows virus preparations by immunodiffusion. Virology 43:480–487
    [Google Scholar]
  28. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. New York:; Cold Spring Harbor Laboratory.:
    [Google Scholar]
  29. Matthews R. E. F. 1982; Plant Virology. New York & London: Academic Press;
    [Google Scholar]
  30. Milner J. J., Jackson A. O. 1983; Characterization of viral-complementary RNA associated with polyribosomes from tobacco infected with sonchus yellow net virus. Journal of General Virology 64:2479–2483
    [Google Scholar]
  31. Rezaian M. A., Heaton L. A., Pederson K., Milner J. J., Jackson A. O. 1983; Size and complexity of polyadenylated RNAs induced in tobacco infected with sonchus yellow net virus. Virology 131:221–229
    [Google Scholar]
  32. Rose J., Schubert M. 1987; Rhabdovirus genomes and their products. In The Rhabdoviruses129–166 Wagner R. R. New York:: Plenum Press;
    [Google Scholar]
  33. Ruther U., Muller-Hill B. 1983; Easy identification of cDNA clones. EMBO Journal 2:1791–1794
    [Google Scholar]
  34. Stenger D.C., Richardson J., Sylvester E. S., Jackson A. O., Morris T. J. 1988; Analysis of sowthistle yellow vein virus specific RNAs in infected hosts. Phytopathology 78:1473–1477
    [Google Scholar]
  35. Stubbs L. L., Grogan R. G. 1963; Necrotic yellows: a newly recognized virus disease of lettuce. Australian Journal of Agricultural Research 14:439–459
    [Google Scholar]
  36. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of SciencesU.S.A. 77:5201–5205
    [Google Scholar]
  37. Toriyama S., Peters D. 1981; Differentiation between broccoli necrotic yellows virus and lettuce necrotic yellows virus by their transcriptase activities. Journal of General Virology 56:59–66
    [Google Scholar]
  38. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesU.S.A. 76:4350–4354
    [Google Scholar]
  39. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., Macleod R. 1972; Classification of rhabdovirus proteins: a proposal. Journal of General Virology 10:1228–1230
    [Google Scholar]
  40. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of SciencesU.S.A. 76:3683–3687
    [Google Scholar]
  41. Wolanski B. S., Chambers T. C. 1971; The multiplication of lettuce necrotic yellows virus. Virology 44:582–591
    [Google Scholar]
  42. Young R. A., Davis R. W. 1983; Efficient isolation of genes by using antibody probes. Proceedings of the National Academy of SciencesU.S.A 80:1194–1198
    [Google Scholar]
  43. Zuidema D., Heaton L. A., Jackson A. O. 1987; Structure of the nucleocapsid protein gene of sonchus yellow net virus. Virology 159:373–380
    [Google Scholar]
  44. Zuidema D., Heaton L. A., Hanau R., Jackson A. O. 1986; Detection and sequence of plus-strand leader RNA of sonchus yellow net virus, a plant rhabdovirus. Proceedings of the National Academy of SciencesU.S.A. 83:5019–5023
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-9-2299
Loading
/content/journal/jgv/10.1099/0022-1317-70-9-2299
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error