@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-9-2287, author = "Karpova, O. V. and Tyulkina, L. G. and Atabekov, K. J. and Rodionova, N. P. and Atabekov*, J. G.", title = "Deletion of the Intercistronic Poly(A) Tract from Brome Mosaic Virus RNA 3 by Ribonuclease H and Its Restoration in Progeny of the Religated RNA 3", journal= "Journal of General Virology", year = "1989", volume = "70", number = "9", pages = "2287-2297", doi = "https://doi.org/10.1099/0022-1317-70-9-2287", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-9-2287", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "BMV", keywords = "ribonuclease", abstract = "Summary The dicistronic genomic RNA 3 of brome mosaic virus (BMV) was used in experiments on site-specific cleavage by RNase H and subsequent religation of large BMV RNA 3 fragments with T4 RNA ligase. BMV RNA 3 was cleaved at the intercistronic poly(A) tract into two fragments: a long (L-BMV 3) 5′-terminal fragment (M r 0·40 × 106) containing the 3a gene, and a short (Sh-BMV 3) fragment (M r 0·28 × 106) containing the coat protein gene and the 3′-terminal tRNA-like tyrosine-accepting structure. Two or three adenylate residues were present at the 5′ end of Sh-BMV 3 and one adenylate at the 3′ end of L-BMV 3. Afterreligation of these RNA fragments BMV RNA 3 was constructed with a deletion including the entire intercistronic poly(A) tract but not the flanking sequences. The religated RNA 3 replicated in wheat plants co-inoculated with BMV RNA 1 and RNA 2. The normal poly(A) tract was restored in progeny BMV RNA 3 during the course of replication.", }