1887

Abstract

Summary

The dicistronic genomic RNA 3 of brome mosaic virus (BMV) was used in experiments on site-specific cleavage by RNase H and subsequent religation of large BMV RNA 3 fragments with T4 RNA ligase. BMV RNA 3 was cleaved at the intercistronic poly(A) tract into two fragments: a long (L-BMV 3) 5′-terminal fragment ( 0·40 × 10) containing the 3a gene, and a short (Sh-BMV 3) fragment ( 0·28 × 10) containing the coat protein gene and the 3′-terminal tRNA-like tyrosine-accepting structure. Two or three adenylate residues were present at the 5′ end of Sh-BMV 3 and one adenylate at the 3′ end of L-BMV 3. Afterreligation of these RNA fragments BMV RNA 3 was constructed with a deletion including the entire intercistronic poly(A) tract but not the flanking sequences. The religated RNA 3 replicated in wheat plants co-inoculated with BMV RNA 1 and RNA 2. The normal poly(A) tract was restored in progeny BMV RNA 3 during the course of replication.

Keyword(s): BMV and ribonuclease
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1989-09-01
2022-08-14
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References

  1. Afanasiev B. N., Rupasov V. V., Fedchenko V. I., Dolja V. V., Atabekov J. G., Chernov V. K., Kozlov YU. V., Bayev A. A. 1986; Primary structure of barley stripe mosaic virus RNA 3 and the generation of RNA 2b and RNA 4.. Dokladȳ Akademii nauk SSSR 290:724–728
    [Google Scholar]
  2. Agranovsky A. A., Dolja V. V., Gorbulev V. G., Kozlov YU. V., Atabekov J. G. 1981; Aminoacylation of barley stripe mosaic virus RNA: polyadenylate-containing RNA has a 3′terminal tyrosine-accepting structure. Virology 113:174–187
    [Google Scholar]
  3. Agranovsky A. A., Dolja V. V., Atabekov J. G. 1983; Differences in polyadenylate length between individual barley stripe mosaic virus RNA species. Virology 129:344–349
    [Google Scholar]
  4. Ahlquist P., Lucknow V., Kaesberg P. 1981; Complete nucleotide sequence of brome mosaic virus RNA 3. Journal of Molecular Biology 153:23–38
    [Google Scholar]
  5. Ahlquist P., French R., Janda M., Loesch Fries L. S. 1984; Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proceedings of the National Academy of SciencesU.S.A 81:7066–7070
    [Google Scholar]
  6. Allison R. F., Janda M., Ahlquist P. 1988; Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus. Journal of Virology 62:3581–3588
    [Google Scholar]
  7. Altenbach S. B., Howell S. H. 1981; Identification of satellite RNA associated with turnip crinkle virus. Virology 112:25–33
    [Google Scholar]
  8. Atabekov K. J., Tyulkina L. G., Karpova O. V., Metelev V. G., Rodionova N. P., Atabekov J. G. 1988; Site-specific enzymatic cleavage of TMV RNA directed by (deoxyribo-ribo) oligonucleotides. FEBS Letters 232:96–98
    [Google Scholar]
  9. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. W. 1986; Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proceedings of the National Academy of SciencesU.S.A. 83:4594–4598
    [Google Scholar]
  10. Dolja V. V., Lunina N. A., Smirnov V. V., Karpov V. A., Hudjakov YU. E., Kozlov YU. V., Baev A. A., Atabekov J. G. 1982; Localization of genes in polycistronic RNAs by the method of oligonucleotide zones on the example of coat protein gene of barley stripe mosaic virus. Dokladȳ Akademii nauk SSSR 265:474–477
    [Google Scholar]
  11. Donis Keller H. 1979; Site specific enzymatic cleavage of RNA. Nucleic Acids Research 7:179–192
    [Google Scholar]
  12. Fowlks E. R., Lee J. F. 1981; Detection and sequence of an internal A-rich T1 oligonucleotide series in brome mosaic viral RNA 3. FEBS Letters 130:32–48
    [Google Scholar]
  13. Fraenkel-Conrat H., Singer B., Tsugita A. 1961; Purification of virus RNA by means of bentonite. Virology 14:54–58
    [Google Scholar]
  14. French R., Ahlquist P. 1987; Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA 3. Journal of Virology 61:1457–1465
    [Google Scholar]
  15. French R., Ahlquist P. 1988; Characterization and engineering of sequences controlling in vivo synthesis of brome mosaic virus subgenomic RNA. Journal of Virology 62:2411–2420
    [Google Scholar]
  16. French R., Janda M., Ahlquist p. 1986; Bacterial gene inserted in an engineered RNA virus: efficient expression in monocotyledonous plant cells. Science 231:1294–1297
    [Google Scholar]
  17. Gupta K.C, Kingsbury D. W. 1984; Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus. Nucleic Acids Research 12:3829–3841
    [Google Scholar]
  18. Kiberstis P. A., Loesch-Fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  19. Kozlov YU. V., Rupasov V. V., Adyshev D. M., Belzhelarskaya S. N., Agranovsky A. A., Mankin A. S., Morozov S. YU., Dolja V. V., Atabekov J. G. 1984; Nucleotide sequence of the 3′-terminal tRNA-like structure in barley stripe mosaic genome. Nucleic Acids Research 12:4001–4009
    [Google Scholar]
  20. Langereis K., Mugnier M. A., Cornelissen B. J. C., Pinck L., Bol J. F. 1986; Variable repeats and poly(A)- stretches in the leader sequences of alfalfa mosaic virus RNA 3. Virology 154:408–414
    [Google Scholar]
  21. Luk D., Masters P. S., Gill D. S., Banerjee A. K. 1987; Intergenic sequences of the vesicular stomatitis virus genome (New Jersey serotype): evidence for two transcription initiation sites within the L gene. Virology 160:88–94
    [Google Scholar]
  22. Marsh L. E., Dreher T. W., Hall T. C. 1988; Mutational analysis of the core and modulator sequences of the BMV RNA 3 subgenomic promoter. Nucleic Acids Research 16:981–995
    [Google Scholar]
  23. Mcmaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of SciencesU.S.A. 74:4835–4838
    [Google Scholar]
  24. Metelev V. G., Rodionova N. P., Chichkova N. V., Atabekov J. G., Bogdanov A. A., Shabarova Z. A., Berzin v., Jansone J., Gren E. J. 1980; Addressed fragmentation of RNA molecules. 11. Determination of a specific cleavage site on MS2 bacteriophage RNA. FEBS Letters 120:17–20
    [Google Scholar]
  25. Mett I. L., Kuzmin E. V., Zajtseva G. N. 1981; Localization of the RNA of two genes on the maxicircular molecule of DNA from the kinetoplast of the zooflagellate Crithidia oncopelti. Doklady Akademii nauk SSSR 261:1274–1276
    [Google Scholar]
  26. Rodionova N. P., Karpova O. V., Metelev V. G., Bogdanova S. L., Shabarova Z. A., Atabekov J. G. 1983; Fragmentation of tobacco mosaic virus RNA: excision of the 3′-proximal region containing the coat protein gene. Molekuljarnaja Biologija 17:809–817
    [Google Scholar]
  27. Shih D., Lane L., Kaesberg P. 1972; Origin of the small component of brome mosaic virus. Journal of Molecular Biology 64:353–362
    [Google Scholar]
  28. Schubert M., Koene J. D., Herman K. C., Lazzarini R. A. 1980; Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L-gene mRNA. Journal of Virology 34:550–559
    [Google Scholar]
  29. Stepanova O. V., Metelev V. G., Chichkova N. V., Smirnov V. D., Rodionova N. P., Atabekov J. G., Bogdanov A. A., Shabarova z. A. 1979; Addressed fragmentation of RNA molecules. FEBS Letters 103:197–199
    [Google Scholar]
  30. Symons R. H. 1985; Viral genome structure. In The Plant Viruses 15781 Francki R. I. B. New York and London: Plenum Press;
    [Google Scholar]
  31. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of SciencesU.S.A. 77:5201–5205
    [Google Scholar]
  32. Tyulkina L. C., Karpova O. V., Rodionova N. P., Atabekov J. G. 1987; Site-specific cleavage and religation of viral RNAs. 1. Infectivity of barley stripe mosiac virus RNA religated from functionally active segments and restoration of the internal poly(A) tract in progeny. Virology 159:312–320
    [Google Scholar]
  33. Tyulkina L. G., Karpova O. V., Atabekov K. I., Rodionova N. P., Atabekov J. G. 1988; Site-specific cleavage of brome mosaic virus RNA 3 in the intercistronic region and restoration of the poly(A) tract in progeny of the religated RNA 3. Doklady Akademii nauk SSSR 300:1489–1490
    [Google Scholar]
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