1887

Abstract

SUMMARY

Human papillomavirus (HPV) type 16 is highly associated with cervical cancer, but it seems that cofactors such as hormones affect its potential oncogenicity. We have analysed the HPV-16 gene expression in response to sex hormones and glucocorticoids in SiHa cells, a human cervical carcinoma cell line. An eightfold induction of HPV-16 transcripts was obtained in oestrogen-treated SiHa cells. Of the five HPV-16 transcripts detected in these cells only the two major ones, the 4·6 kb and the 4·1 kb mRNA species, were affected by oestrogen. Since the five transcripts span the E6 and E7 open reading frames of the HPV-16 genome, these results suggest that the expression of the various transcripts is differentially controlled, as oestrogen regulates only two of them. We have identified in the HPV-16 genome seven different regions with a high degree of similarity to the oestrogen-responsive element consensus sequence (GGTCANNNTGACC). These sequences are located throughout the entire HPV-16 genome. Progesterone or dexamethasone had no detectable stimulatory effect on the various transcripts of HPV-16 in SiHa cells, up to 24 h after treatment of the cells. Since the E6 and E7 open reading frames have been associated with the oncogenic potential of HPV-16, the effect of oestrogen on the transcription of these viral genes may be of biological relevance in the malignant transformation of HPV-16-infected cervical cells.

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1989-08-01
2024-03-29
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References

  1. Aviv H., Leder P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of SciencesU.S.A. 69:1408–1412
    [Google Scholar]
  2. Baker C., Phelps W., Lindgren V., Braun M., Gonda M., Howley P. 1987; Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Journal of Virology 61:962–971
    [Google Scholar]
  3. Boshart M., Gissmann L., Ikenberg H., Scheurlen W., Kleinheinz A., Zur hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:151–157
    [Google Scholar]
  4. Brinton L. 1986; Current epidemiological studies - emerging hypotheses. Banbury Report 21:17–28
    [Google Scholar]
  5. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  6. Crum C. P., Ikenberg H., Richart R. M., Gissmann L. 1984; Human papillomavirus type 16 and early cervical neoplasia. New England Journal of Medicine 310:880–883
    [Google Scholar]
  7. Dürst M., Gissmann L., Ikenberg H., Zur Hausen H. 1983; A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of SciencesU.S.A. 80:3812–3815
    [Google Scholar]
  8. El awady M. K., Kaplan J. B., O’brian S. J., Burk R. D. 1987; Molecular analysis of integrated human papillomavirus 16 sequences in the cervical cancer cell line SiHa. Virology 159:389–398
    [Google Scholar]
  9. Gloss B., Bernard H. U., Seedorf K., Klock G. 1987; The upstream regulatory region of the human papillomavirus-16 contains an E2 protein independent enhancer which is specific for cervical carcinoma cells and regulated by glucocorticoid hormones. EMBO Journal 6:3735–3743
    [Google Scholar]
  10. Green S., Chambon P. 1986; A superfamily of potentially oncogenic hormone receptors. Nature London: 324615–617
    [Google Scholar]
  11. Green S., Chambon P. 1987; Oestradiol induction of a glucocorticoid responsive gene by a chimaeric receptor. Nature London: 32575–78
    [Google Scholar]
  12. Jantzen H. M., Strahle U., Gloss B., Stewart F., Schmid W., Boshart M., Miksicek R., Schutz G. 1987; Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene. Cell 49:29–38
    [Google Scholar]
  13. Kanda T., Furuno A., Yoshiike K. 1988; Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells. Journal of Virology 62:610–613
    [Google Scholar]
  14. Klein-hitpass L., Kaling M., Ryffel G. U. 1988; Synergism of closely adjacent estrogen responsive elements increases their regulatory potential. Journal of Molecular Biology 201:537–544
    [Google Scholar]
  15. Klock G., Strahle U., Schutz G. 1987; Estrogen and glucocorticoid responsive elements are closely related but distinct. Nature London: 329734–736
    [Google Scholar]
  16. Kumar V., Green S., Stack G., Berry M., Jin J. R., Chambon P. 1987; Functional domains of the human estrogen receptor. Cell 51:941–951
    [Google Scholar]
  17. Martinez E., Givel F., Wahli W. 1987; The estrogen responsive element as an inducible enhancer. DNA sequence requirements and conversion to a glucocorticoid responsive element. EMBO Journal 6:3719–3727
    [Google Scholar]
  18. Miksicek R., Heber A., Schmid W., Danesch U., Posseckert G., Beato M., Schutz G. 1986; Glucocorticoid responsiveness of the transcription enhancer of Moloney sarcoma virus. Cell 46:283–290
    [Google Scholar]
  19. Pater M. M., Pater A. 1985; Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145:313–318
    [Google Scholar]
  20. Pfister H. 1984; Biology and biochemistry of papillomaviruses. Review of Physiology, Biochemistry and Pharmacology 99:111–181
    [Google Scholar]
  21. Phelps W., Yee C., Munger K., Howley P. 1988; The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to adenovirus Ela. Cell 53:539–547
    [Google Scholar]
  22. Schneider A., Hotz M., Gissmann L. 1987; Increased prevalence of human papillomaviruses in the lower genital tract of pregnant women. International Journal of Cancer 40:198–201
    [Google Scholar]
  23. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., Zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature London: 314111–114
    [Google Scholar]
  24. Seedorf K., Krammer G., Dürst M., Suhai S., Rowekamp W. 1985; Human papillomavirus type 16 DNA sequence. Virology 145:181–185
    [Google Scholar]
  25. Smotkin D., Wettstein F. O. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer derived cell line and identification of the E7 protein. Proceedings of the National Academy of SciencesU.S.A. 83:4680–4684
    [Google Scholar]
  26. Strahle U., Klock G., Schutz G. 1987; A DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression. Proceedings of the National Academy of SciencesU.S.A. 84:7871–7875
    [Google Scholar]
  27. Tur-Kaspa R., Burk R., Shaul Y., Shafritz D. 1986; Hepatitis B virus DNA contains a glucocorticoid responsive element. Proceedings of the National Academy of SciencesU.S.A. 83:1627–1631
    [Google Scholar]
  28. Von knebel M., Oltersdorf T., Schwarz E., Gissmann L. 1988; Correlation of modified human papillomavirus early gene expression with altered growth properties in C4-1 cervical carcinoma cells. Cancer Research 48:3780–3786
    [Google Scholar]
  29. Walker P., Germond J. E., Brown L. M., Givel F., Wahli W. 1984; Sequence homologies in the region preceding the transcription initiation site of the liver estrogen responsive vitellogenin and apo-VLDLII genes. Nucleic Acids Research 12:8611–8626
    [Google Scholar]
  30. World Health Organization 1985; Invasive cervical cancer and combined oral contraceptives. British Medical Journal 290:261–264
    [Google Scholar]
  31. Yee C., Krishnan-Hewlett I., Baker C. C., Schlegel R., Howley P. M. 1985; Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. American Journal of Pathology 119:361–366
    [Google Scholar]
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