1887

Abstract

SUMMARY

Negative staining electron microscopy was used to study sucrose gradient-purified preparations of the simian immunodeficiency virus (SIV). Both isolated and aggregated virus particles were observed together with some free-lying virus cores. The cores were 110 nm long and 25 to 50 nm wide and were mainly conical or wedge-like in shape. Surface projections were seen on the envelope membrane of many of the virus particles; the knobs were approximately 6 nm in length, 10 nm wide and from an end-on view they had a Y or triangular-shaped morphology.

Keyword(s): electron microscopy , morphology and SIV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-8-2215
1989-08-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/8/JV0700082215.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-8-2215&mimeType=html&fmt=ahah

References

  1. Chrystie I. L., Almeida J. D. 1988; The morphology of human immunodeficiency virus (HIV) by negative staining. Journal of Medical Virology 25:281–288
    [Google Scholar]
  2. Daniel M. D., Letvin N. L., King N. W., Kannagi M., Sehgal P. K., Hunt R. D., Kanki P. J., Essex M., Desrosiers R. C. 1985; Isolation of a T-cell tropic HTLV-III-like retrovirus from macaques. Science 228:1201–1204
    [Google Scholar]
  3. Daniel M. D., Letvin N. L., Sehgal P. K., Schmidt D. K., Silva D. P., Keith R., Solomon F., Hodi F. S. JR, Ringler D. J., Hunt R. D., King N. W., Desrosiers R. C. 1988; Prevalence of antibodies to three retroviruses in a captive colony of macaque monkeys. International Journal of Cancer 41:601–608
    [Google Scholar]
  4. Essex M., Kanki P. J. 1988; The origins of the AIDS virus. Scientific American 259:44–51
    [Google Scholar]
  5. Gelderblom H. R., Hausmann E. H. S., Ozel M., Pauli G., Koch M. A. 1987; Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 156:171–176
    [Google Scholar]
  6. Gelderblom H. R., Ozel E. H. S., Winkel T., Pauli G., Koch M. A. 1988; Fine structure of human immunodeficiency virus (HIV): immunologicalization of structural proteins and virus-cell relation. Micron and microscopica 19:41–60
    [Google Scholar]
  7. Hockley D. J., Wood R. D., Jacobs J. P., Garrett A. J. 1988; Electron microscopy of human immunodeficiency virus. Journal of General Virology 69:2455–2469
    [Google Scholar]
  8. Hoffman D. A., Banapour B., Levy J. A. 1985; Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. Virology 147:326–335
    [Google Scholar]
  9. Kannagi M., Kiyotaki M., Desrosiers R., Reimann K., King N., Waldron L., Letvin N. 1986; Humoral immune responses to T-cell tropic retroviruses STLV-III in monkeys with experimentally induced AIDS. Journal of Clinical Investigation 78:1229–1236
    [Google Scholar]
  10. Lecatsas G., Taylor M. B., Lyons S. F., Schoub B. D. 1986; Pleomorphism in HTLV-III the AIDS virus. South African Medical Journal 69:793
    [Google Scholar]
  11. Mcgrath M., Witte O., Pincus T., Weissman I. L. 1978; Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity. Journal of Virology 25:923–927
    [Google Scholar]
  12. Palmer E., Martin M., Goldsmith C., Switzer W. 1988; Ultrastructure of human immunodeficiency virus type 2. Journal of General Virology 69:1425–1429
    [Google Scholar]
  13. Sarkar N. H. 1987; Animal virus structure. Perspectives in Medical Virology 3:257–272
    [Google Scholar]
  14. Stannard L. M., Van Der Riet F., De St J., Moodie J. W. 1987; The morphology of human immunodeficiency virus particles by negative staining electron microscopy. Journal of General Virology 68:919–923
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-8-2215
Loading
/content/journal/jgv/10.1099/0022-1317-70-8-2215
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error