The Design and Use of Specific Genetic Probes to Identify Closely Related Bunyaviruses and to Determine the Genotype of Their Recombinants Free

Abstract

SUMMARY

Viruses that are very closely related to each other at the genetic and gene product level can prove difficult to distinguish, although they may differ in phenotype (for example in their viruslence or vector preferences). A chimeric genetic probe has been developed and tested to distinguish the S RNAs of two closely related bunyaviruses, snowshoe hare and La Crosse viruses. The technique is applicable to other RNA species of these two bunyaviruses.

Keyword(s): bunyaviruses , LAC , S RNAs and SSH
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1989-08-01
2024-03-29
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References

  1. Akashi H., Bishop D. H. L. 1983; Comparison of the sequences and coding of La Crosse and snowshoe hare bunyavirus S RNA species. Journal of Virology 45:1155–1158
    [Google Scholar]
  2. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  3. Beaty B. J., Holterman M., Tabachnick W., Shope R. E., Rozhon E. J., Bishop D. H. L. 1981; Molecular basis of bunyavirus transmission by mosquitoes: the role of the M RNA segment. Science 211:1433–1435
    [Google Scholar]
  4. Beaty B. J., Miller B. R., Shope R. E., Rozhon E. J., Bishop D. H. L. 1982; Molecular basis of bunyavirus per os infection of mosquitoes: the role of the M RNA segment. Proceedings of the National Academy of SciencesU.S.A 791295–1297
    [Google Scholar]
  5. Bishop D. H. L., Shope R. E. 1979; Bunyaviridae. In Comprehensive Virology 141–156 Fraenkel-Conrat H, Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  6. Bishop D. H. L., Gould K. G., Akashi H., Clerx-Van Haaster C. M. 1982; The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA. Nucleic Acids Research 10:3703–3713
    [Google Scholar]
  7. Cabradilla C. D. JR, Holloway B. P., Obijeski J. F. 1983; Molecular cloning and sequencing of the La Crosse virus S RNA. Virology 128:463–468
    [Google Scholar]
  8. Calisher C. H. 1983; Taxonomy, classification, and geographic distribution of California serogroup bunyaviruses. Progress in Clinical and Biological Research 123:1–16
    [Google Scholar]
  9. Cash P., Vezza A. C., Gentsch J. R., Bishop D. H. L. 1979; Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide. Journal of Virology 31:685–694
    [Google Scholar]
  10. Clerx-Van Haaster C., Bishop D. H. L. 1980; Analyses of the 3′ terminal sequences of snowshoe hare and La Crosse bunyaviruses. Virology 105:564–574
    [Google Scholar]
  11. Clerx-Van Haaster C. M., Akashi H., Auperin D. D., Bishop D. H. L. 1982; Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species. Journal of Virology 41:119–128
    [Google Scholar]
  12. Clewley J. P., Gentsch J. R., Bishop D. H. L. 1977; Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. Journal of Virology 22:459–468
    [Google Scholar]
  13. Elliott R. M., Lees I. F., Watret G. E., Clark W., Pringle C. R. 1984; Genetic diversity of bunyaviruses and mechanisms of genetic variation. In Mechanisms of Viral Pathogenesis61–76 Cohn A., Suchs T. The Hague: Martinus Nijhoff;
    [Google Scholar]
  14. Endres M. I., Jacoby D. R., Janssen R. S., Gonzalez-Scarano F., Nathanson N. 1989; The large viral RNA segment of the California serogroup bunyaviruses encodes the large viral protein. Journal of General Virology 70:223–228
    [Google Scholar]
  15. Eshita Y., Bishop D. H. L. 1984; The complete sequence of the M RNA of snowshoe hare bunyavirus reveals the presence of internal hydrophobic domains in the viral glycoproteins. Virology 137:227–240
    [Google Scholar]
  16. Fauvel M., Artsob H., Calisher C. H., Davignon L., Chagnon A., Skvorc-Ranko R., Belloncik S. 1980; California group virus encephalitis in three children from Quebec: clinical and serologic findings. Canadian Medical Association Journal 122:60–64
    [Google Scholar]
  17. Fazakerley J. K., Gonzalez-Scarano F., Strickler J., Dietzschold B., Karush F., Nathanson N. 1988; Organization of the middle RNA segment of snowshoe hare bunyavirus. Virology 167:422–432
    [Google Scholar]
  18. Fuller F., Bishop D. H. L. 1982; Identification of virus-coded nonstructural polypeptides in bunyavirus infected cells. Journal of Virology 41:643–648
    [Google Scholar]
  19. Fuller F., Bhown A. S., Bishop D. H. L. 1983; Bunyavirus nucleoprotein, N, and a non-structural protein, NSS, are coded by overlapping reading frames in the S RNA. Journal of General Virology 64:1705–1714
    [Google Scholar]
  20. Gentsch J. R., Bishop D. H. L. 1978; Small viral RNA segment of bunyaviruses codes for the viral nucleocapsid protein. Journal of Virology 28:417–419
    [Google Scholar]
  21. Gentsch J. R., Bishop D. H. L. 1979; M RNA viral segment of bunyaviruses codes for two glycoproteins, G1 and G2. Journal of Virology 30:767–770
    [Google Scholar]
  22. Gentsch J., Bishop D. H. L., Obijeski J. F. 1977; The virus particle nucleic acids and proteins of four bunyaviruses. Journal of General Virology 34:257–268
    [Google Scholar]
  23. Gentsch J. R., Wynne L. R., Clewley J. P., Shope R. E., Bishop D. H. L. 1977; Formation of recombinants between snowshoe hare and La Crosse bunyaviruses. Journal of Virology 24:893–902
    [Google Scholar]
  24. Gentsch J. R., Robeson G., Bishop D. H. L. 1979; Recombination between snowshoe hare and La Crosse bunyaviruses. Journal of Virology 31:707–717
    [Google Scholar]
  25. Gentsch I. R., Rozhon E. J., Klimas R. A., El Said L. H., Shope R. E., Bishop D. H. L. 1980; Evidence from recombinant bunyavirus studies that the M RNA gene products elicit neutralizing antibodies. Virology 102:190–204
    [Google Scholar]
  26. Gonzalez-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1982; Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunyaviruses. Virology 120:42–53
    [Google Scholar]
  27. Gonzalez-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1983; Monoclonal antibodies against the G1 nucleocapsid proteins of La Crosse and Tahyna viruses. Progress in Clinical and Biological Research 123:145–156
    [Google Scholar]
  28. Grady L. J., Srihongse S., Grayson M. A., Deibel R. 1983; Monoclonal antibodies against La Crosse virus. Journal of General Virology 64:1699–1704
    [Google Scholar]
  29. Grady L. J., Sanders M. L., Campbell W. P. 1987; The sequence of the M RNA of an isolate of La Crosse virus. Journal of General Virology 68:3057–3071
    [Google Scholar]
  30. Gundersen C. B., Brown K. L. 1983; Clinical aspects of La Crosse encephalitis: preliminary report. Progress in Clinical and Biological Research 123:169–177
    [Google Scholar]
  31. Iroegbu C. U., Pringle C. R. 1981a; Genetic interactions among viruses of the Bunyamwera complex. Journal of Virology 37:383–394
    [Google Scholar]
  32. Iroegbu C. U., Pringle C. R. 1981b; Genetics of the Bunyamwera complex. In The Replication of Negative Strand Viruses159–165 Bishop D. H. L., Compans R. W. Amsterdam: Elsevier;
    [Google Scholar]
  33. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  34. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning : A Laboratory Manual. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  36. Obijeski J. R., Bishop D. H., Palmer E. L., Murphy F. A. 1976; Segmented genome and nucleocapsid of La Crosse virus. Journal of Virology 20:664–675
    [Google Scholar]
  37. Pringle C. R., Iroegbu C. U. 1982; A mutant identifying a third recombination group in a bunyavirus. Journal of Virology 42:873–879
    [Google Scholar]
  38. Pringle C. R., Lees J. F., Clark W., Elliott R. M. 1984; Genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary DNA probes. Virology 135:244–256
    [Google Scholar]
  39. Rozhon E. J., Gensemer P., Shope R. E., Bishop D. H. L. 1981; Attenuation of virulence of a bunyavirus involving an L RNA defect and isolation of LAC/SSH/LAC and LAC/SSH/SSH reassortants. Virology 111:125–138
    [Google Scholar]
  40. Shope R. E., Rozhon E. J., Bishop D. H. L. 1981; Role of the middle-sized bunyavirus RNA segment in mouse virulence. Virology 114:273–276
    [Google Scholar]
  41. Staden R. 1986; The current status and portability of our sequencing handling software. Nucleic Acids Research 14:217–231
    [Google Scholar]
  42. Thompson W. H., Kalfayan B., Anslow R. O. 1965; Isolation of California encephalitis group virus from a fatal human illness. American Journal of Epidemiology 81:245–253
    [Google Scholar]
  43. Tignor G. H., Burrage T. G., Smith A. L., Shope R. E., Bishop D. H. L. 1983; California serogroup gene structure-function relationships: virulence and tissue tropisms. Progress in Clinical and Biological Research 123:129–138
    [Google Scholar]
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