1887

Journal of General Virology header logo

Volume 70, Issue 8

Molecular Cloning, Sequencing and Expression in of the Bean Yellow Mosaic Virus Coat Protein Gene Free

Abstract

SUMMARY

The sequence of 1015 nucleotides from the 3′ poly(A) tract of the potyvirus bean yellow mosaic virus (BYMV) RNA has been determined from two cDNA clones. This sequence contained a single long open reading frame (ORF) starting upstream of the cloned region. The ORF was expressed as a fusion protein in , and the product was detected by antibodies specific for the coat protein of BYMV. The predicted length of the coat protein gene was 822 nucleotides, corresponding to a 273 amino acid coat protein of 30910. The deduced amino acid sequence of the BYMV coat protein was compared to the chemically determined amino acid composition of purified virion protein, and of protein prepared from trypsin-treated virions. The nucleotide and deduced amino acid sequences were compared to the sequences of the coat protein genes of other potyviruses. The BYMV coat protein gene was found to be 50 to 61% homologous to those of other potyviruses at both the nucleotide and amino acid levels; the greatest variation was between the 5′-proximal one-fifth of the genes. Amino acid sequences and hydrophilicity plots of the different potyvirus coat proteins showed similarities which indicated that the structure of the coat protein is highly conserved; a non-terminal region of variability was predicted to be exposed on the exterior of the virion. A putative cleavage site at a glutamine-serine dipeptide was identified by similarity in context to the cleavage sites of tobacco etch virus and tobacco vein mottling virus coat proteins from the viral polyproteins. The BYMV 3′-terminal non-coding region of 166 nucleotides is followed by a poly(A) tract.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BYMV , coat protein and nucleotide sequence
© Society for General Microbiology 1989
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-8-1961
1989-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/8/JV0700081961.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-8-1961&mimeType=html&fmt=ahah

References

  1. Allison R. F., Sorenson J. C., Kelly M. E., Armstrong F. B., Dougherty W. G. 1985a; Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for synthesis and processing of a polyprotein in potyvirus gene expression. Proceedings of the National Academy of SciencesU.S.A 82:3969–3972
     [Google Scholar]
  2. Allison R. F., Dougherty W. G., Parks T. D., Willis L., Johnston R. E., Kelly M., Armstrong F. B. 1985b; Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147:309–316
     [Google Scholar]
  3. Allison R. F., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
     [Google Scholar]
  4. Argos P., Tucker A. D., Philipson L. 1986; Primary structural relationships may reflect similar DNA replication strategies. Virology 149:208–216
     [Google Scholar]
  5. Bevan M. W., Mason S. E., Goelet P. 1985; Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium. EMBO Journal 4:1921–1926
     [Google Scholar]
  6. Bos L. 1970; Bean yellow mosaic virus. CMI/AAB Descriptions of Plant Viruses40
     [Google Scholar]
  7. Carrington J. C., Dougherty W. G. 1987a; Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. Journal of Virology 61:2540–2548
     [Google Scholar]
  8. Carrington J. C., Dougherty W. G. 1987b; Processing of the tobacco etch virus 49K protease requires autoproteolysis. Virology 160:355–362
     [Google Scholar]
  9. Chang C. -A., Hiebert E., Purcifull D. E. 1988; Analysis of in vitro translation of bean yellow mosaic virus RNA: inhibition of proteolytic processing by antiserum to the 49K nuclear inclusion protein. Journal of General Virology 69:1117–1122
     [Google Scholar]
  10. Close T. J., Christmann J. L., Rodriguez R. L. 1983; M13 bacteriophage and pUC plasmids containing DNA inserts but still capable of β-galactosidase α-complementation. Gene 23:131–136
     [Google Scholar]
  11. Davies J. W., Stanley J., Van kammen A. 1979; Sequence homology adjacent to the 3′-terminal poly(A) of cowpea mosaic virus. Nucleic Acids Research 7:493–500
     [Google Scholar]
  12. Davis R. W., Thomas M., Cameron J., , St JOHN T. P., Scherer S., Padgett R. A. 1980; Rapid DNA isolations for enzymatic and hybridization analysis. Methods in Enzymology 65:404–411
     [Google Scholar]
  13. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
     [Google Scholar]
  14. Domier L. L., Shaw J. G., Rhoads R. E. 1987; Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 158:20–27
     [Google Scholar]
  15. Dougherty W. G., Hiebert E. 1980; Translation of potyviral RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104:183–194
     [Google Scholar]
  16. Dougherty W. G., Allison R. F., Parks T. D., Johnston R. E., Feild M. J., Armstrong F. B. 1985; Nucleotide sequence at the 3′ terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein gene organization. Virology 146:282–291
     [Google Scholar]
  17. Dougherty W. G., Carrington J. C., Cary S. M., Parks T. D. 1988; Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO Journal 7:1281–1287
     [Google Scholar]
  18. Eggenberger A. L., Stark D. M., Beachy R. N. 1989; Nucleotide sequence and expression of a soybean mosaic virus coat protein-coding region and its expression in Escherichia coli, Agrobacterium tumifaciens and tobacco callus. Journal of General Virology 70:1853–1860
     [Google Scholar]
  19. Francki R. I. B., Mclean G. D. 1968; Purification of potato virus X and preparation of infectious ribonucleic acid by degradation with lithium chloride. Australian Journal of Biological Sciences 21:1311–1318
     [Google Scholar]
  20. Goldbach R. 1987; Genome similarities between plant and animal RNA viruses. Microbiological Sciences 4:197–202
     [Google Scholar]
  21. Gough K. H., Azad A. A., Hanna P. J., Shukla D. D. 1987; Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. Journal of General Virology 68:297–304
     [Google Scholar]
  22. Govier D. A., Kassanis B. 1974a; Evidence that a component other than the viral particle is needed for aphid transmission of potato virus Y. Virology 57:285–286
     [Google Scholar]
  23. Govier D. A., Kassanis B. 1974b; A virus-induced component of plant sap needed when aphids acquire potato virus Y from purified preparations. Virology 61:420–426
     [Google Scholar]
  24. Govier D. A., Kassanis B., Pirone T. P. 1977; Partial purification and characterization of the potato virus Y helper component. Virology 78:306–314
     [Google Scholar]
  25. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of SciencesU.S.A 72:3961–3965
     [Google Scholar]
  26. Hammond J., Lawson R. H. 1988; An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissue. Journal of Virological Methods 20:203–217
     [Google Scholar]
  27. Hari V. 1981; The RNA of tobacco etch virus: further characterization and detection of protein linked to RNA. Virology 112:391–399
     [Google Scholar]
  28. Hari V., Siegel A., Rozek C., Timberlake W. E. 1979; The RNA of tobacco etch virus contains poly(A). Virology 92:568–571
     [Google Scholar]
  29. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
     [Google Scholar]
  30. Helfman D. M., Feramisco J. R., Fiddes J. C., Thomas G. P., Hughes S. H. 1983; Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. Proceedings of the National Academy of SciencesU.S.A 80:31–35
     [Google Scholar]
  31. Helfman D. M., Feramisco J. R., Fiddes J. C., Thomas G. P., Hughes S. H. 1984; Immunological screening of cDNA expression libraries. Focus 611–5 Gaithersburg: Bethesda Research Laboratories;
     [Google Scholar]
  32. Hellmann G. M., Thornbury D. W., Hiebert E., Shaw J. G., Pirone T. P., Rhoads R. E. 1983a; Cell-free translation of tobacco vein mottling virus RNA. II. Immunoprecipitation of products by antisera to cylindrical inclusion, nuclear inclusion, and helper component proteins. Virology 124:434–444
     [Google Scholar]
  33. Hellmann G. M., Shahabuddin M., Shaw J. G., Rhoads R. E. 1983b; Molecular cloning of DNA complementary to tobacco vein mottling virus RNA. Virology 128:210–220
     [Google Scholar]
  34. Hellmann G. M., Hiremath S. T., Shaw J. G., Rhoads R. E. 1986; Cistron mapping of tobacco vein mottling virus. Virology 151:159–171
     [Google Scholar]
  35. Hellmann G. M., Shaw J. G., Rhoads R. E. 1988; In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-coded protease. Virology 163:554–562
     [Google Scholar]
  36. Hiebert E., Tremaine J. H., Ronald W. P. 1984; The effects of limited proteolysis on the amino acid composition of five potyviruses and on the serological reaction and peptide map of tobacco etch virus capsid protein. Phytopathology 14:311–416
     [Google Scholar]
  37. Hollings M., Brunt A. A. 1981; Potyviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis731–807 Kurstak E. Amsterdam: Elsevier/North-Holland;
     [Google Scholar]
  38. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of SciencesU.S.A 78:3824–3828
     [Google Scholar]
  39. Hurtt S. S. 1987; Detection and comparison of electrophorotypes of hibiscus chlorotic ringspot virus. Phytopathology 77:845–850
     [Google Scholar]
  40. Jordan R. L., Hammond J. 1986; Analysis of antigenic specificity of monoclonal antibodies to several potyviruses. Phytopathology 76:1091
     [Google Scholar]
  41. Larson R., Messing J. 1983; Apple II computer software for DNA and protein sequence data analysis. DNA 231–35
     [Google Scholar]
  42. Leary J. J., Brigati D. J., Ward D. C. 1983; Rapid and sensitive colorimetric method for visualising biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: bio-blots. Proceedings of the National Academy of SciencesU.S.A 80:4045–1049
     [Google Scholar]
  43. Lomonossoff G. P., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
     [Google Scholar]
  44. Lomonossoff G. P., Shanks M., Evans D. 1985; The structure of cowpea mosaic virus replicative form RNA. Virology 144:351–362
     [Google Scholar]
  45. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  46. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
     [Google Scholar]
  47. Moghal S. M., Francki R. I. B. 1976; Towards a system for the identification and classification of potyviruses. I. Serology and amino acid composition of six distinct viruses. Virology 73:350–362
     [Google Scholar]
  48. Nagel J., Hiebert E. 1985; Complementary DNA and expression of the papaya ringspot potyvirus sequences encoding capsid protein and a nuclear inclusion-like protein in Escherichia coli. Virology 143:435–441
     [Google Scholar]
  49. Powell ABEL P., Nelson R. S., De B., Hoffmann K., Rogers S. G., Fraley R. T., Beachy R. N. 1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743
     [Google Scholar]
  50. Proudfoot N. J., Brownlee G. G. 1976; 3′ non-coding region sequences in eukaryotic messenger RNA. Nature, London 263:211–214
     [Google Scholar]
  51. Randles J. W., Da Vies C., Gibbs A. J., Hatta T. 1980; Amino acid composition of capsid protein as a taxonomic criterion for classifying the atypical S strain of bean yellow mosaic virus. Australian Journal of Biological Sciences 33:245–254
     [Google Scholar]
  52. Ravelonandro M., Varveri C., Delbos R., Dunez J. 1988; Nucleotide sequence of the capsid protein gene of plum pox potyvirus. Journal of General Virology 69:1509–1516
     [Google Scholar]
  53. Renart J., Reiser J., Stark G. P. 1979; Transfer of proteins from gels to diazobenzyloxymethyl paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National Academy of SciencesU.S.A 76:3116–3120
     [Google Scholar]
  54. Rosner R., Raccah B. 1988; Nucleotide sequence of the capsid protein gene of potato virus Y (PVY),. Virus Genes 1:255–260
     [Google Scholar]
  55. Ruther V., Koenen M., Otto K., Muller-Hill B. 1981; pUR222, a vector for cloning and rapid sequencing of DNA. Nucleic Acids Research 9:4087–4098
     [Google Scholar]
  56. Sawicki D. L., Gomatos P. J. 1976; Replication of Semliki Forest virus: polyadenylate in plus-stranded RNA and polyuridylate in minus-stranded RNA. Journal of Virology 20:446–464
     [Google Scholar]
  57. Shukla D. D., Inglis A. S., Mckern N. M., Gough K. H. 1986; Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152:118–125
     [Google Scholar]
  58. Shukla D. D., Gough K. H., Ward C. W. 1987; Coat protein of potyviruses. 3. Comparison of amino acid sequences of the coat proteins of four Australian isolates of sugarcane mosaic virus. Archives of Virology 96:59–74
     [Google Scholar]
  59. Shukla D. D., Strike P. M., Tracy S. L., Gough K. H., Ward C. W. 1988; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
     [Google Scholar]
  60. Smith D. R., Cabro J. M. 1980; Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Research 8:2255–2274
     [Google Scholar]
  61. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
     [Google Scholar]
  62. Vance V. B., Beachy R. N. 1984; Translation of soybean mosaic virus RNA in vitro. evidence of proteolytic processing. Virology 132:271–281
     [Google Scholar]
  63. Van emmelo J., Ameloot P., Plaetinck G., Fiers W. 1984; Controlled synthesis of the coat protein of satellite tobacco necrosis virus in Escherichia coli. Virology 136:32–40
     [Google Scholar]
  64. Van Wezenbeek P., Verver J., Harmsen J., Vos P., Van Kammen A. 1983; Primary structure and organization of the middle-component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
     [Google Scholar]
  65. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
     [Google Scholar]
  66. Wengler G., Wengler G., Gross H. D. 1982; Terminal sequences of Sindbis virus-specific nucleic acids: identity in molecules synthesized in vertebrate and insect cells and characteristic properties of the replicative form RNA. Virology 123:273–283
     [Google Scholar]
  67. Yeh S. D., Gonsalves D. 1985; Translation of papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical inclusion protein, and amorphous inclusion protein. Virology 143:260–271
     [Google Scholar]
  68. Yogo Y., Wimmer E. 1973; Poly(A) and poly(U) in poliovirus double-stranded RNA. Nature New Biology 242:171–174
     [Google Scholar]
  69. Yogo Y., Teng M. H., Wimmer E. 1974; Poly(U) in poliovirus minus RNA is 5′ terminal. Biochemical and Biophysical Research Communications 61:1101–1109
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-8-1961
Loading
Molecular Cloning, Sequencing and Expression in Escherichia coli of the Bean Yellow Mosaic Virus Coat Protein Gene
J. Gen. Virol. 70, 1961 (1989); https://doi.org/10.1099/0022-1317-70-8-1961
/content/journal/jgv/10.1099/0022-1317-70-8-1961
/content/journal/jgv/10.1099/0022-1317-70-8-1961
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error