1887

Abstract

SUMMARY

Infection of the human B cell line JOK-1 with herpes simplex virus type 1 persisted over a period of more than 12 months (to date). Although limited cytopathic effects were seen, viral infection did not lead to extinction of the culture. Infectious centre assays, performed at various times after infection, revealed that only a small proportion of cells (1 to 10%) produced infectious virus particles. However, immunofluorescence studies showed that at any given time considerably more cells than calculated by infectious centre assays contained the immediate early viral protein ICP4 and expressed viral glycoproteins. These observations were confirmed by hybridization analyses which revealed the presence of viral DNA even in cells not producing infectious particles. Since no evidence for the involvement of interferon could be found, some other so far unknown intrinsic property of the cells must be responsible for the restriction of virus replication and/or maturation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-7-1907
1989-07-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/7/JV0700071907.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-7-1907&mimeType=html&fmt=ahah

References

  1. Anderson L. C, Galmberg C. G., Janson S. E., Vuopio P., Lehtonen E. 1983; Establishment and characterization of a hairy cell leukemia cell line JOK-1. In Leukemic Markers299 Krapp W. New York: Academic Press;
    [Google Scholar]
  2. Biswal N., Ko U., Akman S., Ross D. D., Pollak A., Cimino E. 1983; Persistence of herpes simplex virus type 2 genome in a human leukemic cell line. Biochimica et biophysica acta 740:271–281
    [Google Scholar]
  3. Brahic M., Haase A. T. 1978; Detection of viral sequences of low reiteration frequency by in situ hybridization. Proceedings of the National Academy of SciencesU.S.A. 75:6125–6129
    [Google Scholar]
  4. Brahic M., Stroop W. G., Beringer I. R. 1981; Theiler’s virus persists in glial cells during demyelinating diseases. Cell 26:123–128
    [Google Scholar]
  5. Braun R. W., Teute H. K., Kirchner H., Munk K. 1984; Replication of herpes simplex virus in human T-lymphocytes: characterization of the viral target cell. Journal of Immunology 132:914–919
    [Google Scholar]
  6. Cummings P. J., Lakomy R. J., Rinaldo C. R. 1981; Characterization of herpes simplex virus persistence in a human T lymphoblastoid cell line. Infection and Immunity 34:817–827
    [Google Scholar]
  7. Domke-Opitz I., Poberschin P., Mittnacht S., Kirchner H. 1987; Role of interferon in persistent infection of macrophages with herpes simplex virus. Virology 159:306–311
    [Google Scholar]
  8. Floyd R., Glasser R., Vonka V., Benyesh-Melnick M. 1971; Studies on the growth of herpes simplex virus in lymphoblastoid cells. Acta virologica 15:133–142
    [Google Scholar]
  9. Hammer S. M., Richter B. S., Hirsch M. S. 1981; Activation and suppression of herpes simplex virus in a human T lymphoid cell line. Journal of Immunology 127:144–148
    [Google Scholar]
  10. Henle W., Henle G., Zur Hausen H. 1969; Effect of herpes simplex virus in cultured Burkitt tumor cells and its failure to influence the Epstein-Barr virus carrier state. Cancer Research 29:489–494
    [Google Scholar]
  11. Kirchner H., Schroeder C. H. 1979; Replication of herpes simplex virus in human B-lymphocytes stimulated by Epstein-Barr virus. Intervirology 11:61
    [Google Scholar]
  12. Kirchner H., Kleinicke C., Northoff H. 1977; Replication of herpes simplex virus in human peripheral blood T lymphocytes. Journal of General Virology 37:647–649
    [Google Scholar]
  13. Leinbach S. S., Summers W. C. 1979; Herpes simplex virus type 1 infection and isogenic Epstein-Barr virus genome-negative and -positive Burkitt’s lymphoma-derived cell lines. Journal of Virology 30:248–254
    [Google Scholar]
  14. Levine M., Goldin A. L., Glorioso J. C. 1980; Persistence of herpes simplex virus genes in cells of neuronal origin. Journal of Virology 35:203–210
    [Google Scholar]
  15. Pülm W., Knippers R. 1985; Transfection of mouse fibroblast cells with a promotorless herpes simplex virus thymidine kinase gene: number of integrated gene copies and structure of single and amplified gene sequence. Molecular and Cellular Biology 5:295–304
    [Google Scholar]
  16. Rinaldo C. R., Richters B. S., Black P. H., Hirsch M. S. 1979; Persistent infection of human lymphoid and myeloid cell lines with herpes simplex virus. Infection and Immunity 25:521–525
    [Google Scholar]
  17. Robey W. G., Graham B. J., Harris C. L., Madden M. J., Pearson G. R., Vande Woude G. F. 1976; Persistent herpes simplex virus infections established in two Burkitt lymphoma derived cell lines. Journal of General Virology 32:51–62
    [Google Scholar]
  18. Roumillat L. F., Florino P. M., Caplan D. D., Lukert P. D. 1980; Analysis and characterization of herpes simplex virus after its persistence in a lymphoblastoid cell line for 15 months. Infection and Immunity 29:671–677
    [Google Scholar]
  19. Shivers B. D., Schachter B. S., Pfaff D. 1986; In situ hybridization for the study of gene expression in the brain. Methods in Enzymology 124:497–510
    [Google Scholar]
  20. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate early protein ICP4. Infection and Immunity 34:684–692
    [Google Scholar]
  21. Stroop W. G., Rock D. L., Fraser N. W. 1984; Localization of herpes simplex virus in the trigeminal and olfactory systems of the mouse central nervous system during acute and latent infections by in situ hybridization. Laboratory Investigation 51:27–38
    [Google Scholar]
  22. Teute H. R., Braun R., Kirchner H., Becker H., Munk K. 1983; Replication of herpes simplex virus in human T-lymphocytes. Intervirology 20:32–41
    [Google Scholar]
  23. Thiele K., Kirchner H. 1988; Effects of different interferons on the replication of herpes simplex virus in human T-lymphocytes. Journal of Interferon Research 8:507–515
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-7-1907
Loading
/content/journal/jgv/10.1099/0022-1317-70-7-1907
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error