1887

Abstract

SUMMARY

cytoplasmic polyhedrosis virus (BmCPV) strain H produces many hexahedral polyhedra (inclusion bodies) in the cytoplasm of insect midgut epithelial cells. The mutant A strain, however, produces polyhedra in the nucleus. We determined cDNA sequences of the polyhedrin genes, the smallest of the 10 genome segments, of these two strains. The polyhedrin genes of the H and A strains were 944 bp long, and encoded polypeptides of 248 amino acids ( 28500) and 252 amino acids ( 29000), respectively. The extra four amino acid residues at the carboxy terminus of the strain A polyhedrin (Arg-Leu-Leu-Val) were the result of a single nucleotide substitution at an opal stop codon (TGA → CGA). A further amino acid substitution of the histidine residue at position 101 (His → Tyr) was seen. The carboxy-terminal extension revealed a considerable similarity to the consensus amino acid sequence of the DNA-binding domain of many DNA-binding proteins. We discuss the relationship between the intracellular localization of polyhedrins and mutations in their amino acid sequences.

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/content/journal/jgv/10.1099/0022-1317-70-7-1885
1989-07-01
2024-06-19
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References

  1. Almendral J. M., Huebsch D., Blundell P. A., Macdonald-Bravo H., Bravo R. 1987; Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins. Proceedings of the National Academy of SciencesU.S.A. 84:1575–1579
    [Google Scholar]
  2. Arella M., Lavallec C., Belloncik S., Furuichi Y. 1988; Molecular cloning and characterization of CPV polyhedrin and its viable deletion mutant genes. Journal of Virology 62:211–217
    [Google Scholar]
  3. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W., Joklik W. K. 1982; Cloning the double- stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of SciencesU.S.A. 79:7644–7648
    [Google Scholar]
  4. Chou P. Y., Fasman G. D. 1978; Empirical predictions of protein conformation. Annual Review of Biochemistry 47:251–276
    [Google Scholar]
  5. Dingwall C., Laskey R. 1986; Protein import into the cell nucleus. Annual Review of Cell Biology 2:367–390
    [Google Scholar]
  6. Hayashi Y., Bird F. T. 1970; The isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth Orgyia leucostigma (Smith). Canadian Journal of Microbiology 16:695–701
    [Google Scholar]
  7. Hukuhara T., Yamaguchi K. 1973; Ultrastructural investigation on a strain of a cytoplasmic polyhedrosis with nuclear inclusions. Journal of Invertebrate Pathology 22:6–13
    [Google Scholar]
  8. Kalderon D., Richardson W. D., Markham A. F., Smith A. E. 1984a; Sequence requirements for nuclear localization of SV40 large-T antigen. Nature, London 311:33–38
    [Google Scholar]
  9. Kalderon D., Roberts B. L., Richardson W. D., Smith A. E. 1984b; A short amino acid sequence able to specify nuclear location. Cell 39:499–509
    [Google Scholar]
  10. Kawase S., Yamaguchi K. 1974; A polyhedrosis virus forming polyhedra in midgut-cell nucleus of the silkworm, Bombyx mori. II. Chemical nature of the virion. Journal of Invertebrate Pathology 24:106–111
    [Google Scholar]
  11. Kuchino Y., Nishimura S., Smith R. E., Furuichi Y. 1982; Homologous terminal sequences in the double-stranded RNA genome segments of cytoplasmic polyhedrosis virus of the silkworm, Bombyx mori . Journal of Virology 44:538–543
    [Google Scholar]
  12. Lanford R. E., Butel J. S. 1984; Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell 37:801–813
    [Google Scholar]
  13. Mori H., Sasaki T., Minobe Y., Miyajima S., Kawase S. 1987; Difference of proteins from inclusion bodies formed in the nucleus and cytoplasm of the CPV-infected midgut in the silkworm, Bombyx mori . Journal of Invertebrate Pathology 50:26–32
    [Google Scholar]
  14. Payne C. C., Rivers C. F. 1976; A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. Journal of General Virology 33:71–85
    [Google Scholar]
  15. Shoji S., Parmeree D. C., Wade R. D., Kumar S., Ericsson L. H., Walsh K. A., Neurath H., Long G., Demaille J. G., Fischer A. W., Titani K. 1981; Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP dependent protein kinase. Proceedings of the National Academy of SciencesU.S.A. 78:848–851
    [Google Scholar]
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