1887

Abstract

SUMMARY

Bovine herpesvirus type 4 proteins were identified by PAGE of [S]methionine- or [H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain were used to identify 29 proteins, ten of which were glycosylated. All of these glycoproteins belonged to the viral envelope and a 140K nonglycosylated protein appeared to be the major nucleocapsid protein. The MAbs were classified into two groups. The first group precipitated three glycoproteins of 150K, 120K and 51K. The 120K and 51K glycoproteins were linked by disulphide bonds and the 150K glycoprotein was linked to the others by non-covalent bonds. The second group precipitated a different 120K glycoprotein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-7-1743
1989-07-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/7/JV0700071743.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-7-1743&mimeType=html&fmt=ahah

References

  1. Bruck C., Mathot S., Portetelle D., Berte C., Franssen J. D., Herion P., Burny A. 1982; Monoclonal anti-bodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp 51. Virology 122:342–352
    [Google Scholar]
  2. Castrucci G., Frigeri F., Cilli V., Donnelli G., Ferrari M., Chicchini V., Bordoni V. 1986; A study of herpesvirus isolated from dairy cattle with a history of reproductive disorders. Comparative Immunology, Microbiology and Infectious Diseases 9:13–21
    [Google Scholar]
  3. Dubuisson J., Thiry E., Thomas I., Coignoul F., Bublot M., Van Heule G., Dekegel D., Pastoret P.-P. 1987; Infection expérimentale de taureaux par injection intratesticulaire d’une souche de bovid herpesvirus 4 isolée d’un cas d’orchite. Annales de médecine vétérinaire 131:37–48
    [Google Scholar]
  4. Dubuisson J., Thiry E., Thalasso F., Bublot M., Pastoret P.-P. 1988; Biological and biochemical comparison of bovid herpesvirus-4 strains. Veterinary Microbiology 16:339–349
    [Google Scholar]
  5. Dubuisson J., Thiry E., Bublot M., Sneyers M., Boulanger D., Guillaume J., Pastoret P.-P. 1989; Production and characterization of monoclonal antibodies to bovid herpesvirus-4. Veterinary Microbiology in press
    [Google Scholar]
  6. Ehlers B., Buhk H.-J, Ludwig H. 1985; Analysis of bovine cytomegalovirus genome structure: cloning and mapping of the monomeric polyrepetitive DNA unit, and comparison of European and American strains. Journal of General Virology 66:55–68
    [Google Scholar]
  7. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgG1; IgG1a and IgG2a immunoglobulins from mouse serum using Protein A–Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  8. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K.-O., Kaplan A. S. 1984; Characterization of the envelope proteins of pseudorabies virus. Journal of Virology 52:583–590
    [Google Scholar]
  9. Herring A. J., Sharp J. M. 1984; Protein blotting: the basic method and its role in viral diagnosis. In Recent Advances in Virus Diagnosis115–124 McNulty M. S., McFerran J. B. The Hague: Martinus Nijhoff;
    [Google Scholar]
  10. Kit S., Kit M., Ichimura H., Crandell R., McConnell S. 1986; Induction of thymidine kinase activity by viruses with group B DNA genomes: bovine cytomegalovirus (bovine herpesvirus 4). Virus Research 4:197–212
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  12. Misra V., Blumenthal R. M., Babiuk L. A. 1981; Proteins specified by bovine herpesvirus type-1 (infectious bovine rhinotracheitis virus). Journal of Virology 40:367–378
    [Google Scholar]
  13. Seppälä I., Sarvas H., Peterfy F., Makela O. 1981; The four subclasses of IgG can be isolated from mouse serum by using Protein A-Sepharose. Scandinavian Journal of Immunology 14:335–342
    [Google Scholar]
  14. Spear P. G. 1985; The herpesvirus glycoproteins. In The Herpesviruses 3315–347 Roizman B. New York: Plenum Press;
    [Google Scholar]
  15. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 19:143–159
    [Google Scholar]
  16. Stevely W. S. 1975; Virus-induced proteins in pseudorabies-infected cells. II. Proteins of the virion and nucleocapsid. Journal of Virology 16:944–950
    [Google Scholar]
  17. Storz J., Ehlers B., Todd W. J., Ludwig H. 1984; Bovine cytomegaloviruses: identification and differential properties. Journal of General Virology 65:697–706
    [Google Scholar]
  18. Theodoridis A. 1978; Preliminary characterization of viruses isolated from cases of epididymitis and vaginitis in cattle. Onderstepoort Journal of Veterinary Research 45:187–195
    [Google Scholar]
  19. Thiry E., Pastoret P. Y, , Dessy-Doize C., Hanzen C., Calberg-Bacq C. M., Dagenais L., Vindevogel H., Ectors F. 1981; Réactivation d’un herpèsvirus en culture de cellules testiculaires prélévees chez un taureau atteint d’orchite et d’azoospermie. Annales de medecine veterinaire 125:207–214
    [Google Scholar]
  20. Thiry E., Bublot M., Dubuisson J., Pastoret P.-P. 1989; Bovine herpesvirus-4 infections of cattle. In Herpesvirus Diseases of Cattle, Horses and Pigs. Developments in Veterinary Virology96–115 Wittman G. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  21. Todd W. J., Storz J. 1983; Morphogenesis of a cytomegalovirus from an American bison affected with malignant catarrhal fever. Journal of General Virology 64:1025–1030
    [Google Scholar]
  22. Van Drunen Little-Van Den Hurk S., Van Der Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A. 1984; Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology 135:466–479
    [Google Scholar]
  23. Van Drunen Little-Van Den Hurk S., Van Den Hurk J. V., Babiuk L. A. 1985; Topographical analysis of bovine herpesvirus type-1 glycoproteins: use of monoclonal antibodies to identify and characterize functional epitopes. Virology 144:216–227
    [Google Scholar]
  24. Wellemans G., Van Opdenbosch E., Mammerickx M. 1986; Inoculation expérimentale du virus LVR 140 (herpès bovin IV) à des vaches gestantes et non gestantes. Annales de recherche vétérinaire 17:89–94
    [Google Scholar]
  25. Zweig M., Heilman C. J., Rabin H., Hampar B. 1980; Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. Journal of Virology 35:644–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-7-1743
Loading
/content/journal/jgv/10.1099/0022-1317-70-7-1743
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error