Molecular Analysis of Double-stranded RNA Free

Abstract

SUMMARY

A detailed analysis was made of dsRNA molecules present in (cultivated mushroom) affected and non-affected by La France disease occurring in The Netherlands. A specific pattern of 10 major dsRNA molecules was always associated with affected strains but non-affected strains also contained at least one of the dsRNAs. Cross-hybridization under stringent conditions showed that the dsRNAs were all unique.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1613
1989-06-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061613.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1613&mimeType=html&fmt=ahah

References

  1. Field L. J., Bruenn J. N., Chang T.-H., Pinhasi O., Koltin Y. 1983; Two Ustilago maydis viral dsRNAs of different size code for the same product. Nucleic Acids Research 11:2765–2787
    [Google Scholar]
  2. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proceedings of the National Academy of Sciences, U.S.A 55:1504–1511
    [Google Scholar]
  3. Hicks R. G. T., Haughton K. L. 1986; Detection of double-stranded RNA in shake cultures of Agaricus bisporus affected by ‘La France’ disease. Transactions of the British Mycological Society 86:579–584
    [Google Scholar]
  4. Maniatis T., Fritsch E. F., Sambrook L. 1982 Molecular Cloning . A Labora iory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Marino R., Saksena K. N., Schuler M., Mayfield J. E., Lemke P. A. 1976; Double-stranded ribonucleic acid in Agaricus bisporus . Applied and Environmental Microbiology 31:432–438
    [Google Scholar]
  6. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  7. Passmore E. L., Frost R. R. 1979; The detection and occurrence of virus-like particles in extracts of mushroom sporophores [Agaricus hortensis (Cooke) Pilat]. Phytopathologische Zeitschrift 95:346–363
    [Google Scholar]
  8. Romaine C. P. 1987; A review of virus disease with reference to hybrid mushrooms. Mushroom News 35:14–21
    [Google Scholar]
  9. Ross R. C., Brown G. A., Romaine C. P. 1986; Recent experience in detecting viral double-stranded RNA in commercial mushroom crops and its effect on yield. In Cultivating Edible Fungi: Proceedings of the International Symposium on Scientific and Technical Aspects of Cultivating Edible Fungi (IMS86)321–329 Wuest P. J., Royse D. J., Beelman R. B. Amsterdam: Elsevier;
    [Google Scholar]
  10. Tartaglia J., Paul C. P., Fulbright D. W., Nuss D. L. 1986; Structural properties of double-stranded RNAs associated with biological control of chestnut blight fungus. Proceedings of the National Academy of Sciences, U.S.A 83:9109–9113
    [Google Scholar]
  11. Tautz D., Renz M. 1983; An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
  12. Thiele D. J., Hannig E. M., Leibowitz M. J. 1984; Genome structure and expression of a defective interfering mutant of the killer virus of yeast. Virology 137:20–31
    [Google Scholar]
  13. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  14. Van Zaayen Z. 1979; Mushroom viruses. In Viruses and Plasmids in Fungi239–324 Lemke P. A. New York: Marcel Dekker;
    [Google Scholar]
  15. Wach M. P., Romaine P. C. 1983; Mushroom viruses-incidence, impact and control. Mushroom News 31:5–6
    [Google Scholar]
  16. Wach M. P., Sriskantha A., Romaine C. P. 1987; Double-stranded RNAs associated with La France disease of the commercial mushroom. Phytopathology 77:1321–1325
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1613
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1613
Loading

Data & Media loading...

Most cited Most Cited RSS feed