A temperature-sensitive mutant (13) of herpes simplex virus type 2 (HSV-2) has a mutation which causes thermolability of the virion. This mutation lies within the gene encoding a virion structural protein of 65K which is known to stimulate immediate early transcription (the trans-inducing factor, 65K). The results presented here show that the structural role of 65K is essential. The electrophoretic mobility of the 65K encoded by 13 and a revertant of 13 differed from that of the wild-type HSV-2 parent. Two monoclonal antibodies directed against 65K were shown to react with two different epitopes on this polypeptide, one of which was altered by the mutation in 13. No differences were observed in the phosphorylation status of 65K from mutant- and wild-type-infected cells.

Keyword(s): HSV-2 and structural gene

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