1887

Abstract

SUMMARY

Three monoclonal antibodies (MAbs) have been raised against cell membrane-derived herpes simplex virus type 1 glycoprotein C (gC-1). By using different DNA constructs of gC-1 expressed in the sites recognized by these antibodies could be assigned to a peptide in the more hydrophobic and probably non-glycosylated middle third of the gC-1 molecule. This peptide segment corresponds to a 571 bp segment on the gC-1 gene located between the I and the I restriction sites. None of the three MAbs interfered with the binding of the human serum complement component C3b to gC, which has been shown to be rather glycosylation-dependent. Since the data of other groups have suggested that antibodies directed against all regions of gC-1 inhibit C3b binding to gC-1, whereas our results suggest that the central part of gC-1 is not actually involved in C3b-binding activity, it can be inferred that the N- and C-terminal segments are involved in C3b binding by gC-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1571
1989-06-01
2022-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061571.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1571&mimeType=html&fmt=ahah

References

  1. Amann E., Bröker M., Wurm F. 1984a; Expression of herpes simplex virus type 1 glycoprotein C antigens in Escherichia coli.. Gene 32:203–215
    [Google Scholar]
  2. Amann E., Broker M., Wurm F. 1984b; High level production in E. coli of hybrid proteins expressing parts of coding sequences of HSV glycoproteins.. In Modern Approaches to Vaccines183–187
    [Google Scholar]
  3. Chanock R. M., Lerner R. A. New York: Cold Spring Harbor Laboratory;
  4. Campadelli-Fiume G., Poletti L., Dall’olio F., Serafini-Cessi F. 1982; Infectivity and glycoprotein processing of herpes simplex virus type 1 grown in a ricin-resistant cell line deficient in N-acetylglycosaminyl transferase I.. Journal of Virology 43:1061–1071
    [Google Scholar]
  5. Dowbenko D. J., Lasky L. A. 1984; Extensive homology between herpes simplex virus type 2 glycoprotein F gene and the herpes simplex virus type 1 glycoprotein C gene.. Journal of Virology 51:154–163
    [Google Scholar]
  6. Draper K. G., Costa R. H., Lee G.-Y., Spear P. G., Wagner E. K. 1984; Molecular basis of the glycoprotein C-negative phenotype of herpes simplex virus type 1 macroplaque strain.. Journal of Virology 51:578–585
    [Google Scholar]
  7. Fazekas De ST, Groth A., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics.. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  8. Friedman H. M., Cohen G. H., Eisenberg R. J., Seidel C. A., Cinés D. B. 1984; Glycoprotein C of herpes simplex virus 1 acts as a receptor for the C3b complement component on infected cells.. Nature, London 309:633–635
    [Google Scholar]
  9. Friedman H. M., Glorioso J. C., Cohen G. H., Hastings J. C., Harris S. L., Eisenberg R. J. 1986; Binding of complement component C3b to glycoprotein gC of herpes simplex virus type 1 : mapping of gC-binding sites and demonstration of conserved C3b binding in low passage clinical isolates.. Journal of Virology 60:470–475
    [Google Scholar]
  10. Fries L. F., Friedman H. M., Cohen G. H., Eisenberg R. J., Hammer C. H., Frank M. M. 1986; Glycoprotein C of herpes simplex virus 1 is an inhibitor of the complement cascade.. Journal of Immunology 137:1636–1641
    [Google Scholar]
  11. Frink R. J., Eisenberg R., Cohen G., Wagner E. K. 1983; Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C.. Journal of Virology 45:634–647
    [Google Scholar]
  12. Görtz J., Brake B., Härle-Grupp V., Falke D. 1984; Replication of HSV-1 in murine peritoneal macrophages: comparison of various virus strains with different properties.. Archives of Virology 79:173–187
    [Google Scholar]
  13. Guarente L., Lauer G., Robert T. M., Ptashne M. 1980; Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globin.. Cell 20:543–553
    [Google Scholar]
  14. Hammer C. H., Wirtz G. H., Renfer L., Gresham H. D., Tack B. F. 1981; Large scale isolation of functionally active components of the human complement system.. Journal of Biological Chemistry 256:3995–4006
    [Google Scholar]
  15. Holland T. C., Homa F. L., Marlin S. D., Levine M., Glorioso J. 1984; Herpes simplex virus type 1 glycoprotein C-negative mutants exhibit multiple phenotypes, including secretion of truncated glycoproteins.. Journal of Virology 51:566–574
    [Google Scholar]
  16. Homa F. L., Purifoy D. J. M., Glorioso J. C., Levine M. 1986; Molecular basis of the glycoprotein C-negative phenotypes of herpes simplex virus type 1 mutants selected with a virus-neutralizing monoclonal antibody.. Journal of Virology 58:281–289
    [Google Scholar]
  17. Hope R. G., Palfreyman J., Suh M., Marsden H. S. 1982; Sulphated glycoproteins induced by herpes simplex virus.. Journal of General Virology 58:399–415
    [Google Scholar]
  18. Johnson D. C., Spear P. G. 1983; O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus.. Cell 32:987–997
    [Google Scholar]
  19. Koenen M., Rüther U., Müller-Hill B. 1982; Immunoenzymatic detection of expressed gene fragments cloned in the lacZ gene of E. coli . EMBO Journal 1:509–512
    [Google Scholar]
  20. Kubota Y., Gaither T. A., Cason J., O’shea J. J., Lawley T. J. 1987; Characterization of the C3 receptor induced by herpes simplex virus type 1 infection of human epidermal, endothelial, and A431 cells.. Journal of Immunology 138:1137–1142
    [Google Scholar]
  21. Mcnearney T. A., Odell C., Holers V. M., Spear P. G., Atkinson J. P. 1987; Herpes simplex virus glycoproteins gC-1 and gC-2 bind to the third component of complement and provide protection against complement-mediated neutralization of viral infectivity.. Journal of Experimental Medicine 166:1525–1535
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Marlin S. D., Holland T. C., Levine M., Glorioso J. C. 1985; Epitopes of herpes simplex virus type 1 glycoprotein gC are clustered in two distinct antigenic sites.. Journal of Virology 53:128–136
    [Google Scholar]
  24. Olofsson S., Sjöblom L., Lundström M., Jeansson S., Lycke E. 1983; Glycoprotein C of herpes simplex virus type 1 : characterization of O-linked oligosaccharides.. Journal of General Virology 64:2735–2747
    [Google Scholar]
  25. Petzer A. L., Schulz T. F., Stauder R., Eigentler A., Myones B. L., Dierich M. P. 1988; Structural and functional analysis of CR2/EBV receptor by means of monoclonal antibodies and limited tryptic digestion.. Immunology 63:47–53
    [Google Scholar]
  26. Rüther U., Müller-Hill B. 1983; Easy identification of cDNA clones.. EMBO Journal 2:1791–1794
    [Google Scholar]
  27. Schmitt M., Mussel H. H., Hammann K. P., Scheiner O., Dierich M. P. 1981; Role of β1H for the binding of C3b-coated particles to human lymphoid and phagocytic cells.. European Journal of Immunology 11:739–745
    [Google Scholar]
  28. Schröder C. H., Engler H., Kirchner H. 1981; Protection of mice by an apathogenic strain of HSV-1 against lethal infection by a pathogenic strain of HSV-1.. Journal of General Virology 52:159–161
    [Google Scholar]
  29. Sjöblom L., Lundström M., Sjogren-Jansson E., Glorioso J. C., Jeansson S., Olofsson S. 1987; Demonstration and mapping of highly carbohydrate-dependent epitopes in the herpes simplex virus type 1-specified glycoprotein C.. Journal of General Virology 68:545–554
    [Google Scholar]
  30. Smiley M. L., Friedman H. M. 1985; Binding of complement component C3b to glycoprotein C is modulated by sialic acid on herpes simplex virus type 1-infected cells.. Journal of Virology 55:857–861
    [Google Scholar]
  31. Smiley M. L., Hoxie J. A., Friedman H. M. 1985; Herpes simplex virus type 1 infection of endothelial, epithelial and fibroblast cells induces a receptor for C3b.. Journal of Immunology 134:2673–2678
    [Google Scholar]
  32. Wenske E. A., Bratton M. W., Courtney R. J. 1982; Endo-β-N-acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC.. Journal of Virology 44:241–248
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1571
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1571
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error