We expressed the bovine herpesvirus type 1 (BHV-1) glycoproteins, gI and gIII, in bovine cells using a bovine papillomavirus vector. The proteins expressed by these cells had the same Mr as the native BHV-1 proteins and monoclonal antibodies detected no differences in their antigenic structure. Cells expressing gI were infected with either BHV-1 or herpes simplex virus type 1 (HSV-1). The number of plaques in gI-expressing cells was similar to that seen with normal fibroblasts infected with BHV-1 or HSV-1. However, BHV-1 or HSV-1 plaques produced in gI-expressing cells were smaller and darker than those seen in normal fibroblasts indicating an interference with cell-to-cell transmission or cellular lysis. Virus growth curves and [35S]methionine labelling of BHV-1-infected gI-expressing cells showed no difference in virus production, virus protein synthesis or cellular protein shutdown when compared to BHV-1-infected normal cells. This led us to conclude that the gI protein may interfere with a cellular protein(s) responsible for the cytopathic effects of BHV-1 infection. Cells expressing gIII were fully susceptible to BHV-1 infection.
AliM. A.,
ButcherM.,
GhoshH. P.1987; Expression and nuclear envelope localization of biologically active fusion glycoprotein gB of herpes simplex virus in mammalian cells using cloned DNA. Proceedings of the National Academy of Sciences, U.S.A 84:5675–5679
AnonymousA.1980; Soft Agar Cloning techniques. In Cloning Techniques68Proceedings of the Cell Cloning Seminar, 12 to 14 November 1980, at the W. Alton Jones Cell Science CenterLake PlacidNew York, U.S.A
BabiukL. A.,
L’LtalienJ.,
Van Drunen Littel-Van Den HurkS.,
ZambT.,
LawmanM. J. P.,
HughesG.,
GiffordG. A.1987; Protection of cattle from bovine herpesvirus type I (BHV-1) infection by immunization with individual viral glycoproteins. Virology 159:57–66
Campadelli-FiumeG.,
AvitabileE.,
FiniS.,
StirpeD.,
ArsenakisM.,
RoizmanB.1988; Herpes simplex virus glycoprotein D is sufficient to induce spontaneous pH-independent fusion in a cell line that constitutively expresses the glycoprotein. Virology 166:598–602
ConwayM.,
MorganA.,
MackettM.1989; Expression of Epstein-Barr virus membrane antigen gp340/220 in mouse fibroblasts using a bovine papillomavirus vector. Journal of General Virology 70:729–734
EidenM.,
NewmanM.,
FisherA. G.,
MannD. L.,
HowleyP. M.,
ReitzM. S.1985; Type 1 human T-cell leukemia virus small envelope protein expressed in mouse cells by using a bovine papilloma virus-derived shuttle vector. Molecular and Cellular Biology 5:3320–3324
FitzpatrickD. R.,
Bielefeldt OhmannH.,
RedmondM. J.,
CamposM.,
BabiukL. A.1988a; Molecular mimicry by bovine herpesvirus type 1. Abstract No. 300 69th Annual Meeting of Conference of Research Workers in Animal Disease, 14 and 15 November 1988 Chicago, Illinois, U.S.A:
FitzpatrickD. R.,
ZambT.,
ParkerM. D.,
Van Drunen Littel-Van Den HurkS.,
BabiukL. A.,
LawmanM. I. P.1988b; Expression of bovine herpesvirus 1 glycoproteins gI and gIII in transfected murine cells. Journal of Virology 62:4239–4248
FreshneyR. I.1983; Disaggregation of the tissue and primary culture. In Culture of Animal Cells: A Manual of Basic Techniques104–110 New York: A.R. Liss;
GerberJ. D.,
MarronA. E.,
KuceraC. J.1978; Local and systemic cellular and antibody immune response of cattle to infectious bovine rhinotracheitis virus vaccine administered intranasally or intramuscularly. American Journal of Veterinary Research 39:753–760
IsraelB. A.,
MarshallR. L.,
LetchworthG. J.III1988; Epitope specificity and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines. Vaccine 6:349–356
LuptonH. W.,
ReedD. E.1980; Evaluation of experimental subunit vaccines for infectious bovine rhinotracheitis. American Journal of Veterinary Research 41:383–390
LuskyM.,
BergL.,
WeiherH.,
BotchanM.1983; Bovine papilloma virus contains an activator of gene expression at the distal end of the early transcription unit. Molecular and Cellular Biology 3:1108–1122
MckercherD. G.1978; Disease incidence and epidemiology — the situation in the U.S.A. In Respiratory Diseases in Cattle71–83MartineW. B.
The Hague: Martinus Nijhoff;
MarkwellM. A. K.,
FoxC. F.1978; Surface specific iodination of membrane proteins of viruses and eukaryotic cells using 1,3,4,6-tetrachloro-3α,6α-diphenylgrycouril. Biochemistry 17:4807–4817
MarshallR. L.,
RodriguezL. L.,
LetchworthG. J.III1986; Characterization of the envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods. Journal of Virology 57:745–753
MatthiasP. D.,
BernardH. U.,
ScottA.,
BradyG.,
Hashimoto-GotohT.,
SchutzG.1983; A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells. EMBO Journal 2:1487–1492
MisraV.,
NelsonR.,
SmithM.1988; Sequence of a bovine herpesvirus type-1 glycoprotein gene that is homologous to the herpes simplex gene for the glycoprotein gB. Virology 166:542–549
OkazakiK.,
HondaE.,
MinetomaT.,
KumagaiT.1987; Bovine herpesvirus 1 gp87 mediates both attachment of virions to susceptible cells and hemagglutination. Archives of Virology 97:297–307
SouthernP. J.,
BergP.1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
Van Drunen Littel-Van Den HurkS.,
Van Den HurkJ. V.,
GilchristJ. E.,
MisraV.,
BabiukL. A.1984; Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology 135:466–479
Van Drunen Littel-Van Den HurkS.,
Van Den HurkJ. V.,
BabiukL. A.1985; Topographical analysis of bovine herpesvirus type 1 glycoproteins: use of monoclonal antibodies to identify and characterize functional epitopes. Virology 144:216–227
WangY.,
Stratowa C,
Schaefer-RidderM.,
DoehmerJ.,
HofschneiderP. H.1983; Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector. Molecular and Cellular Biology 3:1032–1039
WhitbeckJ. C.,
BelloL. I.,
LawrenceW. C.1988; Comparison of the bovine herpesvirus 1 gI and the herpes simplex type 1 gB gene. Journal of Virology 62:3319–3327
YatesW. D. G.1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral-bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine 46:225–263
ZambT.1987; Identification of genes encoding the major immunogens of BHV-1: the mapping, subcloning, and expression in foreign systems of the gB, gC and gD gene homologs. Abstract No. 330 68th Annual Meeting of Conference of Research Workers in Animal Disease, 16 and 17 November 1987 Chicago, Illinois, U.S.A: