1887

Abstract

SUMMARY

Glycoprotein gp52 exists within the mature human cytomegalovirus (HCMV) envelope in heterodimeric, disulphide-linked complexes with glycoproteins gp95 and gp130. Biochemical studies involving immunoprecipitations and Western blots have demonstrated that gp52 is the glycoprotein B (gB) homologue of HCMV but that gp95 and gp130 are probably separate gene products. The distribution of this putative gB on extracellular HCMV particles was revealed by high resolution electron microscopy of preparations labelled with a monoclonal antibody, F5, directly coupled to colloidal gold. F5–gold probes, specific for HCMV gp52, bind to the distal end of 12 nm long, slender spikes projecting from virion and dense body envelopes. Labelled spikes were most often present in closely packed, homogeneous clusters and were frequently present on envelope protrusions. The degree of labelling on individual HCMV particles was highly variable. Both the morphology and distribution of HCMV gp52 show strong similarity with that previously reported for the gB of herpes simplex virus. Other morphologically distinct spikes occur in the HCMV envelope but these were not recognized by F5–gold probes.

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/content/journal/jgv/10.1099/0022-1317-70-6-1553
1989-06-01
2024-12-07
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References

  1. Britt W. J. 1984; Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus.. Virology 135:369–378
    [Google Scholar]
  2. Cranage M. P., , Kouzarides T., , Bankier A. T., , Satchwell S., , Weston K., , Tomlinson P., , Barrell B., , Hart H., , Bell S. E., , Minson A. C., Smith G. L. 1986; Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.. EMBO Journal 5:3057–3063
    [Google Scholar]
  3. Farrar G. H., Greenaway P. J. 1986; Characterization of glycoprotein complexes present in human cytomegalovirus envelopes.. Journal of General Virology 67:1469–1473
    [Google Scholar]
  4. Farrar G. H., Oram J. D. 1984; Characterization of the human cytomegalovirus envelope glycoproteins.. Journal of General Virology 65:1991–2001
    [Google Scholar]
  5. Gretch D. R., , Kari B., , Rasmussen L., , Gehrz R. C., Stinski M. F. 1988a; Identification and characterization of three distinct families of glycoprotein complexes in the envelopes of human cytomegalovirus.. Journal of Virology 62:875–881
    [Google Scholar]
  6. Gretch D. R., , Gehrz R. C., Stinski M. F. 1988b; Characterization of a human cytomegalovirus glycoprotein complex (gcl).. Journal of General Virology 69:1205–1215
    [Google Scholar]
  7. Kari B., , Lussenhop N., , Goertz R., , Wabuke-Bunoti M., , Radeke R., Gehrz R. C. 1986; Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.. Journal of Virology 60:345–352
    [Google Scholar]
  8. Law K. M., , Wilton-Smith P., Farrar G. H. 1985; A murine monoclonal antibody recognizing a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex.. Journal of Medical Virology 17:255–266
    [Google Scholar]
  9. Nowak B., , Sullivan C., , Sarnow P., , Thomas R., , Bricout F., , Nicolas I. C., , Fleckenstein B., Levine A. J. 1984; Characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins.. Virology 132:325–338
    [Google Scholar]
  10. Stannard L. M., , Fuller A. O., Spear P. G. 1987; Herpes simplex virus glycoproteins associated with different morphological entities projecting from the virion envelope.. Journal of General Virology 68:715–725
    [Google Scholar]
  11. Stinski M. F. 1976; Human cytomegalovirus : glycoproteins associated with virions and dense bodies.. Journal of Virology 19:594–609
    [Google Scholar]
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