1887

Abstract

SUMMARY

B/Singapore/222/79-like influenza viruses isolated from three patients during the winter of 1981 to 1982 and cultured in either embryonated hens' eggs or MDCK cells were studied. Sequence analysis indicated that the haemagglutinin (HA) genes of the six virus preparations contained at least four distinct HA1 sequences which differed by up to six amino acids. Only one pair of viruses had amino acid differences between the egg- and MDCK cell-derived viral subpopulations and this change did not affect a glycosylation site. Mice infected with previously described recombinant vaccinia viruses expressing either the egg- or MDCK cell-derived HA of B/England/222/82 developed neutralizing antibodies against all of the 1982 type B viruses and were protected against intranasal challenge with these viruses. Therefore, in this model system, the minor sequence variation between the HAs of egg- and MDCK cell-derived influenza B/England/222/82 virus had no detectable effect on the induction of cross-protection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1533
1989-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061533.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1533&mimeType=html&fmt=ahah

References

  1. Anderson L., Hierholzer J., Bingham P., Stone Y. 1986; Microneutralization test for RSV based on an enzyme assay.. Journal of Clinical Microbiology 22:1050–1052
    [Google Scholar]
  2. Bean W. J., Sriram G., Webster R. G. 1980; Electrophoretic analysis of iodine-labeled influenza virus RNA segments.. Analytical Biochemistry 102:228–232
    [Google Scholar]
  3. Berton M. T., Webster R. G. 1985; The antigenic structure of the influenza B virus hemagglutinin: operational and topographical mapping with monoclonal antibodies.. Virology 143:583–594
    [Google Scholar]
  4. Berton M. T., Naeve C. W., Webster R. G. 1984; Antigenic structure of the influenza B virus hemagglutinin: nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies.. Journal of Virology 52:919–927
    [Google Scholar]
  5. Dowdle W. R., Kendal A. P., Noble G. R. 1979; Influenza viruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections585–609 Lennette E. H., Schmidt N. J. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  6. Harmon M. W., Rota P. A., Walls H. H., Kendal A. P. 1988; Antibody response in humans to influenza B host-cell derived variants after vaccination with standard egg-derived vaccine or natural infection.. Journal of Clinical Microbiology 26:333–337
    [Google Scholar]
  7. Katz J., Naeve C., Webster R. 1987; Host cell-mediated variation in H3N2 influenza viruses.. Virology 156:386–395
    [Google Scholar]
  8. Kendal A. P., Noble G. R., Skehel J. J., Dowdle W. R. 1978; Antigenic similarity of influenza A (H1N1) viruses from epidemics in 1977–1978 to “Scandinavian” strains isolated in epidemics of 1950–1951.. Virology 89:632–636
    [Google Scholar]
  9. Krystal M., Elliott R. M., Benz E. W., Young J. F., Palese P. 1982; Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes.. Proceedings of the National Academy of Sciences, U.S.A. 79:4800–4804
    [Google Scholar]
  10. Oxford J. S., Corcoran T., Knott R., Bates J., Bartolomei O., Major D., Newman R. W., Yates P., Robertson J., Webster R. G., Schild G. C. 1987; Serological studies with influenza A (H1N1) viruses cultivated in eggs or in a canine kidney cell line (MDCK).. Bulletin of the World Health Organization 65:181–187
    [Google Scholar]
  11. Patterson S., Oxford J. 1986; Analysis of antigenic determinants on internal and external proteins of influenza virus and identification of antigenic subpopulations of virions in recent field isolates using monoclonal antibodies and immunogold labeling.. Archives of Virology 88:199–202
    [Google Scholar]
  12. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. 1985; Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs.. Virology 143:166–174
    [Google Scholar]
  13. Rota P. A., Shaw M. W., Kendal A. P. 1987; Comparison of the immune response to variant influenza type B hemagglutinin expressed in vaccinia virus.. Virology 161:269–275
    [Google Scholar]
  14. Schild G. C., Oxford J. S., De Jong J. C., Webster R. W. 1983; Evidence for host-cell selection of influenza virus antigenic variants.. Nature, London 303:706–709
    [Google Scholar]
  15. Verhoeyen M., Rompuy L., Jou W., Huylebroeck D., Fiers W. 1983; Complete nucleotide sequence of the influenza B/Singapore/222/79 virus hemagglutinin gene and comparison with the B/Lee/40 hemagglutinin.. Nucleic Acids Research 11:4703–4712
    [Google Scholar]
  16. Walls H., Johansson K., Harmon M., Halonen P., Kendal A. 1986; Time-resolved fluoroimmunoassay with monoclonal antibodies for rapid diagnosis of influenza infections.. Journal of Clinical Microbiology 24:907–912
    [Google Scholar]
  17. Webster R. G., Berton M. T. 1981; Analysis of antigenic drift in the haemagglutinin molecule of influenza B virus with monoclonal antibodies.. Journal of General Virology 54:243–251
    [Google Scholar]
  18. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation.. Nature, ljmdon 289:373–378
    [Google Scholar]
  19. Yamashita M., Krystal M., Fitch W. M., Palese P. 1988; Influenza B virus evolution: co-circulating lineages and comparison of evolutionary pattern with those of influenza A and C viruses.. Virology 163:112–122
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1533
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1533
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error