1887

Abstract

SUMMARY

Human T cell clones were prepared from peripheral blood mononuclear cells from a vaccinated human donor and kept in culture in the presence of rabies virus antigen and growth factors. Phenotypic analysis of the T cell clones revealed expression of the CD3 and CD4 cell surface markers, but not of CD8, consistent with a phenotype of helper/inducer T cells. The rabies virus specificity of the T cell clones was established by virus-specific proliferation in response to the rabies virus Pitman-Moore strain (PM) produced in three different cell substrates. The clones also responded to the rabies virus strains Evelyn-Rokitnicki-Abelseth (ERA) and challenge virus standard (CVS), but not to the rabies virus-related Mokola and Duvenhage-6 virus strains. Proliferative responses of T cell clones required rabies virus antigen to be presented by autologous antigen-presenting cells in association with HLA class II molecules. When cultured with rabies virus antigen, but in the absence of growth factors, some of the T cell clones provided help for an antibody response of rabies virus immune B lymphocytes. Analysis of culture supernatant fluids showed that at least a part of this antibody response was directed against neutralizing antibody-inducing determinants of the viral glycoprotein.

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1989-06-01
2024-03-28
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References

  1. Bunschoten H., Gore M., Claassen I. J. TH. M., Uytdehaag F. G. C. M., Dietzschold B., Wunner W. H., Osterhaus A. D. M. E. 1989; Characterization of a new virus-neutralizing epitope that denotes a sequential determinant on the rabies virus glycoprotein.. Journal of General Virology 70:291–298
    [Google Scholar]
  2. Celis E., Miller R. W., Wiktor T. J., Dietzschold B., Koprowski H. 1986; Isolation and characterization of human T cell lines and clones reactive to rabies virus: antigen specificity and production of interferon-γ.. Journal of Immunology 136:692–697
    [Google Scholar]
  3. Celis E., Ou D., Dietzschold B., Koprowski H. 1988; Recognition of rabies and rabies related viruses by T cells derived from human vaccine recipients.. Journal of Virology 62:3128–3134
    [Google Scholar]
  4. Cho S., Narahara H., Mifune K., Kawai A. 1987; Murine T cell clones directed to rabies virus: isolation and some of their properties.. Journal of General Virology 68:1115–1123
    [Google Scholar]
  5. Cox J. H., Dietzschold B., Schneider L. G. 1977; Rabies virus glycoprotein. II. Biological and serological characterization.. Infection and Immunity 16:754–759
    [Google Scholar]
  6. Lafon M., Wiktor T. I., Macfarlan R. I. 1983a; Antigenic sites on the CVS rabies virus glycoprotein: analysis with monoclonal antibodies.. Journal of General Virology 64:843–851
    [Google Scholar]
  7. Lafon M., Ideler I., Wunner W. H. 1983b; Investigation of the antigenic structure of rabies virus glycoprotein by monoclonal antibodies.. Developments in Biological Standardization 57:219–225
    [Google Scholar]
  8. Lumio J., Hillbom M., Roine R., Ketonen L., Haltia M., Valle M., Neuvonen E., Lahdevirta J. 1986; Human rabies of bat origin in Europe.. Lancet i:378
    [Google Scholar]
  9. Manca F., Kunkl A., Fenoglio D., Fowler A., Sercarz E., Celada F. 1985; Constraints in T–B cell cooperation related to epitope topology on E. coli β-galactosidase. I. The fine specificity of T cells dictates the fine specificity of antibodies directed to conformation-dependent determinants.. European Journal of Immunology 15:345–350
    [Google Scholar]
  10. Mifune K., Takeuchi E., Napiorkowski P. A., Yamada A., Sakamoto K. 1981; Essential role of T cells in the post-exposure prophylaxis of rabies in mice.. Microbiology and Immunology 25:895–904
    [Google Scholar]
  11. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody. A new method of conjugation.. Journal of Histo-chemistry and Cytochemistry 11:1084–1091
    [Google Scholar]
  12. Ozaki S., Berzofsky I. A. 1987; Antibody conjugates mimic specific B cell presentation of antigen: relationship between T and B cell specificity.. Journal of Immunology 138:4133–4142
    [Google Scholar]
  13. Prabhakar B. S., Fischman H. R., Nathanson N. 1981; Recovery from experimental rabies by adoptive transfer of immune cells.. Journal of General Virology 56:25–31
    [Google Scholar]
  14. Scherle P. A., Gerhard W. 1988; Differential ability of B cells specific for external vs. internal influenza virus proteins to respond to help from influenza virus-specific T-cell clones in vivo. Proceedings of the National Academy of Sciences, U.S.A. 85:4446–4450
    [Google Scholar]
  15. Smith J. S. 1981; Mouse model for abortive rabies infection in the central nervous system.. Infection and Immunity 16:297–308
    [Google Scholar]
  16. Turner G. C. 1985; Immune response after rabies vaccination. Basic aspects.. Annales de I’Institut Pasteur/Virologie 136E:453–460
    [Google Scholar]
  17. Uytdehaag F. G. C. M., Osterhaus A. D. M. E., Loggen H. G., Barker R. H. J., Van Asten J. A. A. M., Kreeftenberg J. G., Van Der Marel P., Van Steenis B. 1983; Introduction of antigen-specific antibody response in human peripheral blood lymphocytes in vitro by a dog kidney cell vaccine against rabies virus (DKCV).. Journal of Immunology 131:1234–1239
    [Google Scholar]
  18. Uytdehaag F., Claassen I., Bunschoten H., Loggen H., Ottenhof T., Teeuwsen V., Osterhaus A. 1987; Human anti-idiotypic T lymphocyte clones are activated by autologous anti-rabies virus antibodies presented in association with HLA-DQ molecules.. Journal of Molecular Cell Immunology 3:145–155
    [Google Scholar]
  19. Van Wezel A. L., Van Steenis B., Hannik C. A., Cohen H. 1978; New approaches to the production of concentrated and purified inactivated polio and rabies tissue culture vaccines.. Developments in Biological Standardization 41:159–168
    [Google Scholar]
  20. Wiktor T. J., Koprowski H. 1978; Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants.. Proceedings of the National Academy of Sciences, U.S.A. 75:3938–3942
    [Google Scholar]
  21. Wiktor T. J., Gyorgy E., Schlumberger H. D., Sokol F., Koprowski H. 1973; Antigenic properties of rabies virus components.. Journal of Immunology 110:269–276
    [Google Scholar]
  22. Wiktor T. I., Kamo I., Koprowski H. 1974; In vitro stimulation of rabbit lymphocytes after immunization with live and inactivated rabies vaccines.. Journal of Immunology 112:2013–2019
    [Google Scholar]
  23. Wiktor T. J., Doherty P. C., Koprowski H. 1977; In vitro evidence of cell mediated immunity after exposure of mice to both live and inactivated rabies virus.. Proceedings of the National Academy of Sciences, U.S.A. 74:334–338
    [Google Scholar]
  24. Wiktor T. J., Macfarlan R. I., Reagan K., Dietzschold B., Curtis P., Wunner W. H., Kiney M. P., Lathe R., Lecocq I. P., Mackett M., Moss B., Koprowski H. 1984a; Protection from rabies by vaccinia virus recombinant containing the rabies glycoprotein gene.. Proceedings of the National Academy of Sciences, U.S.A 81:7194–7198
    [Google Scholar]
  25. Wiktor T. J., Macfarlan R. I., Goggin C. M., Koprowski H. 1984b; Antigenic analysis of rabies and Mokola virus from Zimbabwe using monoclonal antibodies.. Developments in Biological Standardization 57:199–211
    [Google Scholar]
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