1887

Abstract

SUMMARY

A mouse monoclonal antibody (MAb) to dengue 4 (DEN-4) virus reacted with the antigen in the nucleus as well as in the cytoplasm of DEN-4-infected mammalian and mosquito cells, as demonstrated by the peroxidase–antiperoxidase staining method. The intranuclear antigen appeared to accumulate at the nucleoli, forming spots, whereas the cytoplasmic antigen appeared to be localized mainly in large perinuclear foci in the infected cells. The MAb-reactive antigen was produced in the presence of actinomycin D, which caused the accumulation in the nucleus to be altered to a dispersed pattern. Radioimmunoprecipitation analysis of [S]methionine-labelled purified virions and Western blot analysis of the antigens prepared from the infected mammalian and mosquito cells showed that the MAb was directed against the DEN-4 virus core protein ( 15·5K). These results indicated that the DEN-4 virus core protein was partially transported, soon after its synthesis in the cytoplasm, into the nucleus and accumulated at the nucleoli.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1409
1989-06-01
2024-02-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061409.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1409&mimeType=html&fmt=ahah

References

  1. Anzai T., Ozaki Y. 1969; Intranuclear crystal formation of poliovirus: electron microscopic observations. Experimental and Molecular Pathology 10:176–185
    [Google Scholar]
  2. Brinton M. A. 1986; Replication of Flaviviruses. In The Togaviridae and Flaviviridae327–374 Schlesinger S., Schlesinger M. J. New York: Plenum Press;
    [Google Scholar]
  3. Buckley A., Gould E. A. 1988; Detection of virus-specific antigen in the nuclei or nucleoli of cells infected with Zika or Langat virus. Journal of General Virology 69:1913–1920
    [Google Scholar]
  4. Clarke D. H., Casals I. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 17:561–573
    [Google Scholar]
  5. Dittmar D., Haines H. G., Castro A. 1980; Monoclonal antibodies specific for dengue virus type 3. Journal of Clinical Microbiology 12:74–78
    [Google Scholar]
  6. Igarashi A. 1978; Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and chikungunya viruses. Journal of General Virology 40:531–544
    [Google Scholar]
  7. Jackson D. A., Caton A. J., Mccready S. J., Cook P. R. 1982; Influenza virus RNA is synthesized at fixed sites in the nucleus. Nature, London 296:366–368
    [Google Scholar]
  8. Kalderon D., Roberts B. L., Richardson W. D., Smith A. E. 1984; A short amino acid sequence able to specify nuclear location. Cell 39:499–509
    [Google Scholar]
  9. Kennet R. H., Denis K. A., Tung A. S., Klinman N. R. 1978; Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Current Topics in Microbiology and Immunology 81:77–91
    [Google Scholar]
  10. Kos K. A., Osborne B. A., Goldsby R. A. 1975; Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B. Journal of Virology 15:913–917
    [Google Scholar]
  11. Murphy F. A., Harrison A. K., Gary G. W., Whitfield S. G., Forrester F. T. 1968; St Louis encephalitis virus infection of mice: electron microscopic studies of central nervous system. Laboratory Investigation 19:652–662
    [Google Scholar]
  12. Ng M. L., Pedersen J. S., Toh B. H., Westaway E. G. 1983; Immunofluorescent sites in Vero cells infected with the flavivirus Kunjin. Archives of Virology 78:177–190
    [Google Scholar]
  13. Okuno Y., Fukunaga T., Srisupaluck S., Fukai K. 1979; A modified PAP (peroxidase-anti-peroxidase) staining technique using sera from patients with dengue hemorrhagic fever (DHF): 4 step PAP staining technique. Biken Journal 22:131–135
    [Google Scholar]
  14. Okuno Y., Igarashi A., Fukai K. 1978; Neutralization tests for dengue and Japanese encephalitis viruses by the focus reduction method using peroxidase-anti-peroxidase staining. Biken Journal 21:137–147
    [Google Scholar]
  15. Schoefl G. I. 1964; The effect of actinomycin D on the fine structure of the nucleolus. Journal of infrastructure Research 10:224–243
    [Google Scholar]
  16. Sever J. L. 1962; Application of a microtechnique to viral serological investigations. Journal of Immunology 88:320–329
    [Google Scholar]
  17. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. 1971; The proteins of Japanese encephalitis virus. Virology 44:108–124
    [Google Scholar]
  18. Shulman M., Wilde C. D., Kohler G. 1978; A better line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  19. Stollar V. 1969; Studies on the nature of dengue viruses: IV. The structural proteins of type 2 dengue virus. Virology 39:426–438
    [Google Scholar]
  20. Trent D. W. 1977; Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. Journal of Virology 22:608–618
    [Google Scholar]
  21. Trent D. W., Naeve C. W. 1980; Biochemistry and replication. In St Louis Encephalitis159–198 Monath T. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  22. Westaway E. G., Ng M. L. 1980; Replication of flaviviruses: separation of membrane translation sites of Kunjin virus proteins and of cell protein. Virology 106:107–122
    [Google Scholar]
  23. Yasuzumi G., Tsubo L, Sugihara R., Nakai Y. 1964; Analysis of the development of Japanese B encephalitis (JBE) virus. I. Electron microscope studies of microglia infected with JBE virus. Journal of Ultrastructure Research 11:213–229
    [Google Scholar]
  24. Zhao B., Mackow E., Buckler-White A., ., Markoff L., Chanock R. M., Lai C., Makino Y. 1986; Cloning full- length dengue type 4 viral DNA sequences: sof genes coding for structural proteins. Virology 155:77–88
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1409
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1409
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error