1887

Abstract

SUMMARY

A mouse genomic segment containing three new members of the murine interferon alpha (MuIFN-) gene family was isolated from a fibroblastic cosmid library. A 4 kb RI fragment contained a new MuIFN- gene named MuIFN-8. The nucleotide sequence of the coding and flanking regions of this gene showed a high level of homology to those of known members of the MuIFN- family. Transient expression of the MuIFN-8 gene in COS cells and oocyte translation of transcripts both led to a biologically active protein. The antiviral activity was neutralized by monoclonal and polyclonal MuIFN- antibodies. Although the 5′ flanking sequence shows features characteristic of an IFN regulatory region, the MuIFN-8 gene is not expressed in murine fibroblasts treated with Newcastle disease virus or poly(I)·poly(C).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1381
1989-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061381.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1381&mimeType=html&fmt=ahah

References

  1. Bisat F., Raj N. B. K., Pitha P. M. 1988; Differential and cell type specific expression of murine alpha-interferon genes is regulated on the transcriptional level.. Nucleic Acids Research 16:6067–6083
    [Google Scholar]
  2. Daugherty B., Martin-Zanca D., Kelder B., Collier K., Seamans T. C., Hotta K., Pestka S. 1984; Isolation and bacterial expression of a murine alpha leukocyte interferon gene.. Journal of Interferon Research 4:635–643
    [Google Scholar]
  3. Dinter H., Hauser H. 1987; Cooperative interaction of multiple DNA elements in the human interferon-β promoter.. European Journal of Biochemistry 166:103–109
    [Google Scholar]
  4. Dion M., Vodjdani G., Doly J. 1986; Sequence and expression of a novel murine interferon alpha gene homology with enhancer elements in the regulatory region of the gene.. Biochemical and Biophysical Research Communications 138:826–834
    [Google Scholar]
  5. Fujita T., Ohno S., Yasumitsu H., Taniguchi T. 1985; Delimitation and properties of DNA sequences required for the regulated expression of human interferon-β gene.. Cell 41:489–496
    [Google Scholar]
  6. Gluzman Y. 1981; SV40-transformed simian cells support the replication of early SV40 mutants.. Cell 23:175–182
    [Google Scholar]
  7. Goodbourn S., Maniatis T. 1988; Overlapping positive and negative regulatory domains of the human β-interferon gene.. Proceedings of the National Academy of Sciences, U.S.A. 85:1447–1451
    [Google Scholar]
  8. Goodbourn S., Zinn K., Maniatis T. 1985; Human β-interferon gene expression is regulated by an inducible enhancer element.. Cell 41:509–520
    [Google Scholar]
  9. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. 1971; Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.. Nature, London 233:177–182
    [Google Scholar]
  10. Hattori M., Sakaki Y. 1986; Dideoxy sequencing method using denatured plasmid templates.. Analytical Biochemistry 152:232–238
    [Google Scholar]
  11. Hiscott J., Cantell K., Weissmann C. 1984; Differential expression of human interferon genes.. Nucleic Acids Research 12:3727–3746
    [Google Scholar]
  12. Kelley K. A., Pitha P. M. 1985a; Characterization of a mouse interferon gene locus. I. Isolation of a cluster of four interferon genes.. Nucleic Acids Research 13:805–823
    [Google Scholar]
  13. Kelley K. A., Pitha P. M. 1985b; Characterization of a mouse gene locus. II. Differential expression of interferon genes.. Nucleic Acids Research 13:825–839
    [Google Scholar]
  14. Kuhl D., De La Fuente J., Chaturvedi M., Parimoo S., Ryals J., Meyer F., Weissmann C. 1987; Reversible silencing of enhancers by sequences derived from the human IFN-α promoter.. Cell 50:1057–1069
    [Google Scholar]
  15. Le Roscouet D., Vodjdani G., Lemaigre-Dubreuil Y., Tovey M. G., Latta M., Doly J. 1985; Structure of a murine alpha interferon pseudogene with a repetitive R-type sequence in the 3′ flanking region.. Molecular and Cellular Biology 5:1343–1348
    [Google Scholar]
  16. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  17. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.. Gene 19:269–276
    [Google Scholar]
  18. O’hare K., Bbnoist C., Breathnach R. 1981; Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.. Proceedings of the National Academy of Sciences, U.S.A. 78:1527–1531
    [Google Scholar]
  19. Ragg H., Weissmann C. 1983; Not more than 117 base pairs of 5′-flanking sequence are required for inducible expression of a human IFN-α gene.. Nature, London 303:439–442
    [Google Scholar]
  20. Ryals J., Dierks P., Ragg H., Weissmann C. 1985; A 46-nucleotide promoter segment from an IFN-α gene renders an unrelated promoter inducible by virus.. Cell 41:497–507
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  22. Seif I., De Maeyer-Guignard J. 1986; Structure and expression of a new murine interferon-alpha gene: MuIFN-α9.. Gene 43:111–121
    [Google Scholar]
  23. Shaw G., Kamen R. 1986; A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation.. Cell 46:659–667
    [Google Scholar]
  24. Shaw G. D., Boll W., Taira H., Mantei N., Lengyel P., Weissmann C. 1983; Structure and expression of cloned murine IFN-α genes.. Nucleic Acids Research 11:555–573
    [Google Scholar]
  25. Taira H., Broeze R. J., Jayaram B. M., Lengyel P., Hunkapiller M. W., Hood L. E. 1980; Mouse interferons: amino terminal amino acid sequences of various species.. Science 207:528–530
    [Google Scholar]
  26. Tovey M. G., Begon-LOURS J., Gresser I. 1974; A method for large scale production of potent interferon preparations.. Proceedings of the Society for Experimental Biology and Medicine 146:809–815
    [Google Scholar]
  27. Trapman J., Van Heuvel M., De Jonge P., Bosveld I. J., Klaassen P., Zwarthoff E. C. 1988; Structure–function analysis of mouse interferon alpha species: MuIFN-α10, a subspecies with low antiviral activity.. Journal of General Virology 69:67–75 addendum 2701
    [Google Scholar]
  28. Van Der Korput J. A. G. M., Hilkens J., Kroezen V., Zwarthoff E. C., Trapman J. 1985; Mouse interferon alpha and beta genes are linked at the centromere proximal region of chromosome 4.. Journal of General Virology 66:493–502
    [Google Scholar]
  29. Vodjdani G., Le Roscouet D., . Horth M., Perucca-Lostanlen D., Gallien C., Doly J. 1985; Structure of mouse interferon-β cDNA clones: sequence rearrangements in a cloned cDNA.. Biochemistry International 10:495–505
    [Google Scholar]
  30. Weissenbach J., Zeevi M., Landau T., Revel M. 1979; Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates.. European Journal of Biochemistry 98:1–8
    [Google Scholar]
  31. Weissmann C., Weber H. 1986; The interferon genes.. Progress in Nucleic Acid Research and Molecular Biology 33:251–300
    [Google Scholar]
  32. Wetzel R. 1981; Assignment of the disulphide bonds of leukocyte interferon.. Nature, London 289:606–607
    [Google Scholar]
  33. Wigler M., Pellicer A., Silverstein S., Axel R. 1978; Biochemical transfer of single-copy eukaryotic genes using total cellular DNA as donor.. Cell 14:725–731
    [Google Scholar]
  34. Yamamoto Y., Kawade Y. 1980; Antigenicity of mouse interferons: distinct antigenicity of the two L cell interferon species.. Virology 103:80–88
    [Google Scholar]
  35. Zinn K., Di Maio D., Maniatis T. 1983; Identification of two distinct regulatory regions adjacent to the human β-interferon gene.. Cell 34:865–879
    [Google Scholar]
  36. Zwarthoff E. C., Mooren A. T. A., Trapman J. 1985; Organization, structure and expression of murine interferon alpha genes.. Nucleic Acids Research 13:791–804
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1381
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1381
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error