1887

Abstract

Summary

The complete nucleotide sequence of the phytoreovirus rice dwarf virus (RDV) genome segment 9 is presented. It consisted of 1305 nucleotides and had an open reading frame that codes for a putative polypeptide of 351 amino acids. of the protein was calculated to be 38 598. The terminal nucleotides 5′ GGUAAA--GAU 3′ were the same as those of RDV genome segment 10. The fourth nucleotide from the 3′end of an expected conserved sequence was a C rather than the U found in the previously sequenced genome segment 10. A structure similar to the segment-specific inverted repeat of wound tumour virus was also found in the terminal region of segment 9.

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1989-05-01
2023-02-01
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References

  1. Anzola J. V., Xu X., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of SciencesU.S.A 84:8301–8305
    [Google Scholar]
  2. Asamizu T., Summers D., Motika M. B., Anzola J. V., Nuss D. L. 1985; Molecular cloning and characterization of the genome of wound tumor virus: a tumor-inducing plant reovirus. Virology 144:398–409
    [Google Scholar]
  3. Boccardo G., Milne R. G. 1984; Plant reovirus group. CMI/AAB Descriptions of Plant Viruses294
    [Google Scholar]
  4. De Wachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  5. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  6. Jordan R. L., Dodds J. A. 1983; Hybridization of 5′-end-labelled RNA to plant viral RNA in agarose and acrylamide gels. Plant Molecular Biology Reporter 1:31–37
    [Google Scholar]
  7. Kodama T., Suzuki N. 1973; RNA polymerase activity in purified rice dwarf virus. Annals of the Phytopathological Society of Japan 39:251–258
    [Google Scholar]
  8. Kozak M. 1987; An analysis of 5′-noncoding sequence from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8132
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Matsuoka M., Minobe Y., Omura T. 1985; Reaction of antiserum against SDS-dissociated rice dwarf virus and a polypeptide of rice gall dwarf virus. Phytopathology 75:1125–1127
    [Google Scholar]
  11. Meshi T., Ishikawa M., Takamatsu N., Ohno T., Okada Y. 1983; The 5′-terminal sequence of TMV RNA; question on the polymorphism found in vulgare strain. FEBS Letters 162:282–285
    [Google Scholar]
  12. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–77
    [Google Scholar]
  13. Nakata M., Fukunaga K., Suzuki N. 1978; Polypeptide components of rice dwarf virus. Annals of the Phytopathological Society of Japan 44:288–296
    [Google Scholar]
  14. Omura T., Minobe Y., Tsuchizaki T. 1988; Nucleotide sequence of segment S10 of the rice dwarf virus genome. Journal of General Virology 69:227–231
    [Google Scholar]
  15. Rensing U. F. E., Schoenmakers J. G. G. 1973; A sequence of 50 nucleotides from coliphage R17 RNA. European Journal of Biochemistry 33:8–18
    [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A 74:5463–5467
    [Google Scholar]
  17. Smith H. O. 1980; Recovery of DNA from gels. Methods in Enzymology 65:371–380
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Uyeda I., Shikata E. 1982; Ultrastructure of rice dwarf virus. Annals of the Phytopathological Society of Japan 48:295–300
    [Google Scholar]
  20. Uyeda I., Shikata E. 1984; Characterization of RNAs synthesized by the virion-associated transcriptase of rice dwarf virus in vitro. Virus Research 1:527–532
    [Google Scholar]
  21. Uyeda I., Matsumura T., Sano T., Oshima K., Shikata E. 1987; Nucleotide sequence of rice dwarf virus genome segment 10. Proceedings of the Japan Academy 63:227–230
    [Google Scholar]
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