1887

Abstract

Summary

Mouse monoclonal antibodies (MAbs) were prepared to an inhibitor of virus replication (IVR), released from protoplasts or leaf tissue of hypersensitive tobacco plants infected with tobacco mosaic virus. The MAbs were highly specific for IVR and reduced its antiviral activity. Using these MAbs in affinity chromatography enabled the recovery of purified IVR. SDS-PAGE of the immunoaffinity-purified IVR gave a single 23K band. Immunoblots of IVR from extracts of protoplast or leaf tissue also revealed a single 23K band which suggests that protoplast and tissue IVR are closely related.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-5-1293
1989-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/5/JV0700051293.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-5-1293&mimeType=html&fmt=ahah

References

  1. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  2. Gera A., Loebenstein G. 1983; Further studies of an inhibitor of virus replication from tobacco mosaic virus-infected protoplasts of a local lesion-responding tobacco cultivar. Phytopathology 73:111–115
    [Google Scholar]
  3. Gera A., loebenstein G. 1989; Evaluation of antisera to an inhibitor of virus replication (IVR). Journal of Phytopathology in press
    [Google Scholar]
  4. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  5. Loebenstein G., Gera A. 1981; Inhibitor of virus replication released from tobacco mosaic virus infected protoplasts of a local lesion-responding cultivar. Virology 114:132–139
    [Google Scholar]
  6. Moav N., Smorodinsky N. I., Balin B., Witz I. P. 1982; Monoclonal antibodies from mice bearing polyoma virus-induced tumor. Cancer Immunology and Immunotherapy 12:217–224
    [Google Scholar]
  7. Spiegel S., Gera A., Salomon R., Ahl P., Harlap S., Loebenstein G. 1989; Recovery of an inhibitor of virus replication from the intercellular fluid of hypersensitive tobacco infected with tobacco mosaic virus and from non-infected induced resistant tissue. Phytopathology79 in press
    [Google Scholar]
  8. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesU.S.A 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-5-1293
Loading
/content/journal/jgv/10.1099/0022-1317-70-5-1293
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error