1887

Abstract

Summary

Epstein-Barr virus (EBV) DNA sequences were detected in four established monoblast or early monocytic cell lines (CM-S, ROV-S, CV-S and AD-S) obtained from bone marrow of children suffering from maturation defects of haematopoiesis. EBV is present in these cells in a latent state. The viral DNA in these cell lines was analysed by Southern blot hybridization, using a set of cloned EBV DNA fragments from the EBV strain B95-8 as probes. A common spectrum of highly related but distinguishable EBV DNA restriction enzyme sequences was found, suggesting some genomic diversity. Propagation of the cells in long-term culture revealed a gradual decrease of EBV copies per cell in all lines with some minor changes in the restriction pattern of the EBV DNA. These findings demonstrate that human precursor monocyte cells may be susceptible to infection by EBV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-5-1203
1989-05-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/5/JV0700051203.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-5-1203&mimeType=html&fmt=ahah

References

  1. Arrand J. R., Rymo J. E., Walsh J. E., Bjorck E., Lindahl T., Griffin B. E. 1981; Molecular cloning of the complete Epstein–Barr virus genome as a set of overlapping restriction endonuclease fragments. Nucleic Acids Research 9:2999–3014
    [Google Scholar]
  2. Bayliss G. J., Wolf H. 1980; Epstein–Barr virus-induced cell fusion. Nature, London 287:164–165
    [Google Scholar]
  3. Bertolini L., Butler R. H., Revoltella R. P. 1984; Growth factors that stimulate human granulocyte- macrophage colony formation produced by the cell line CM-S. Journal of Cellular Physiology 119:65–70
    [Google Scholar]
  4. Dolyniuk M., Pritchett R., Kieff R. 1976; Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein–Barr virus. Journal of Virology 17:935–949
    [Google Scholar]
  5. Einhorn L., Host A. 1984; Cytomegalovirus infection of human blood cells. Journal of Infectious Diseases 149:207–214
    [Google Scholar]
  6. Epstein M. A., Achong B. G. 1979; The Epstein–Barr virus. In The Epstein–Barr Virus1–20 Epstein M. A., Achong B. G. Berlin: Springer-Verlag;
    [Google Scholar]
  7. Ernberg I., Danell E., Gratama J. W., Oosterveer R., Klein G. 1988; Molecular weight variations of EBN As as a means to identify different Epstein-Barr virus isolates (EB No typing). Journal of Experimental and Clinical Cancer Research 7:98 supplement
    [Google Scholar]
  8. Fingeroth I. D., Weis J. J., Tedder T. F., Strominger J. S., Biro P. A., Fearon D. T. 1984; Epstein–Barr virus receptor of human B lymphocytes is the C3d receptor CR2. Proceedings of the National Academy of SciencesU.S.A 81:4510–4514
    [Google Scholar]
  9. Frade R., Barel M., Ehlin-Enriksson B., Klein G. 1985; Gpl40, the C3d receptor of human B lymphocytes, is also the Epstein–Barr virus receptor. Proceedings of the National Academy of SciencesU.S.A 82:1490–1493
    [Google Scholar]
  10. Griffin B. E., Karran L. 1984; Immortalization of monkey epithelial cells by specific fragments of Epstein–Barr virus DNA. Nature, London 309:78–82
    [Google Scholar]
  11. Heller M., Van Santen V., Kieff E. 1982; Simple repeat sequence in Epstein–Barr virus DNA is transcribed in latent and productive infections. Journal of Virology 44:311–320
    [Google Scholar]
  12. Henle G., Henle W. 1966; Immunofluorescence in cells derived from Burkitt’s lymphoma. Journal of Bacteriology 91:1248–1256
    [Google Scholar]
  13. Jones J. F., Shurin S., Abramowskiy C., Tubbs R. R., Sciotto C. G., Wahl R., Sands J., Gottam D., Katz B. Z., Sklar J. 1988; T cell lymphomas containing Epstein–Barr viral DNA in patients with chronic Epstein–Barr virus infections. New England Journal of Medicine 318:733–741
    [Google Scholar]
  14. Katamine S., Otsu M., Tada K., Tsuchiya S., Sato T., Ishida N., Honio T., Ono Y. 1984; Epstein–Barr virus transforms precursor B cell even before immunoglobulin gene rearrangements. Nature, London 309:369–372
    [Google Scholar]
  15. Klein G., Giovanella B., Westman A., Stehlin J. S., Mumford D. 1975; An EBV-genome-negative cell line established from an American Burkitt lymphoma: receptor characteristics, EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5:319–334
    [Google Scholar]
  16. Lemon S. M., Hutt L. M., Shaw J. E., Li J. L. H., Pagano J. S. 1977; Replication of EBV in epithelial cells during infectious mononucleosis. Nature, London 268:268–270
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982; Extraction, purification and analysis of mRNA from eukariotic cells. In Molecular Cloning: A Laboratory Manual187–199 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Miller G., Grogan E., Heston L., Robinson J., Smith D. 1981; Epstein–Barr viral DNA: infectivity for human placental cells. Science 212:452–455
    [Google Scholar]
  19. Monaco G., Vigneti E., Lancieri D., Cornaglia-Ferraris P., Lambertenghi-Deliliers G., Revoltella R. P. 1982; Induction of monocyte-macrophage differentiation in a new diploid line of human hemopoietic cells (CM-S). Cancer Research 42:4182–4189
    [Google Scholar]
  20. Nonoyama M., Pagano J. S. 1973; Homology between Epstein–Barr virus DNA and viral DNA from Burkitt’s lymphoma and nasopharingeal carcinoma determinated by DNA-DNA reassociation kinetics. Nature, London 242:44–47
    [Google Scholar]
  21. Raab-Traub N., Flynn K. 1986; The structure of the termini of the Epstein–Barr virus as a marker of clonal cellular proliferation. Cell 47:883–889
    [Google Scholar]
  22. Raab-Traub N., Dambaugh T., Kieff E. 1980; DNA of Epstein–Barr virus. VIII. B95-8, the previous prototype, is an unusual deletion derivative. Cell 22:257–267
    [Google Scholar]
  23. Ragona G., Frezza D., Fruscalzo A., Jucker R., Rossi N., Raimondi E., Barletta C, De Carli L., Calef E. 1986; Studies on host-virus genome relationship in Epstein–Barr virus immortalized lymphoblastoid cell lines. Cell Biology International Reports 10:315–321
    [Google Scholar]
  24. Ranelletti F. O., Starace G., Piantelli M., Lambertenghi-Deliliers G., Revoltella R. P. 1983; Glucocorticoid receptors and cortico sensitivity in a human clonal monocytic cell line, CM-SM. Journal of Cellular Physiology 116:329–335
    [Google Scholar]
  25. Ranelletti F. O., Carbone A., Piantelli M., Revoltella R. P. 1986; Glucocorticoid receptors and corticosensitivity in the human monocytic cell line CM-S: changes during phorbol ester-induced differentiation. Cellular Immunology 99:95–107
    [Google Scholar]
  26. Reedman B. M., Klein G. 1973; Cellular localization of an Epstein–Barr virus (EBV)-associated complement-fixing antigen in producer and non producer lymphoblastoid cell lines. International Journal of Cancer 11:499–520
    [Google Scholar]
  27. Revoltella R. P., Vigneti E., Park M., Simoni G., Romitti L. 1986; Spontaneous in vitro malignant transformation of a monocytic cell line (CM-S) from a patient with congenital hypoplastic anemia. Cancer Journal 1:111–117
    [Google Scholar]
  28. Rice G. P. A., Schrier R. D., Oldstone M. B. A. 1984; Cytomegalovirus infects human lymphocytes and monocytes: virus expression restricted to immediate-early gene products. Proceedings of the National Academy of SciencesU.S.A 81:6134–6138
    [Google Scholar]
  29. Rocchi G., Ragona G., Revoltella R. P. 1984; EBV expression in human non-lymphoid hematopoietic cell lines. Progress in Medical Virology 30:129–138
    [Google Scholar]
  30. Romitti L., Revoltella R. P., Vigneti E., Simoni G. 1986; Chromosome changes in human monocytic cell lines with in vitro spontaneous malignant transformation. Human Genetics 74:256–261
    [Google Scholar]
  31. Rosen A., Gergely P., Jondal M., Klein G. 1977; Polyclonal Ig production after Epstein–Barr virus infection of human lymphocytes in vitro. Nature, London 267:52–54
    [Google Scholar]
  32. Sambuy Y., Spagnoli G., Vigneti E., Revoltella R. P. 1985; Ultrastructural studies of spontaneous in vitro transformation of cultured monocyte-macrophage cells from a patient with congenital hypoplastic anemia. Cancer Research 45:1392–1401
    [Google Scholar]
  33. Sixbey J. W., Vesterinen E. H., Nedrud J. G., Raab-Traub N., Walton L. A., Pagano J. S. 1983; Replication of Epstein–Barr virus in human epithelial cells infected in vitro. Nature, London 306:480–483
    [Google Scholar]
  34. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  35. Staczek S., Steplewsky Z., Weinmamn R., Klein G., Koprowski H. 1981; Epstein–Barr virus in nontumorigenic and tumorigenic nasopharingeal carcinoma (NPC) somatic cell hybrids. Journal of Cellular Physiology 107:391–398
    [Google Scholar]
  36. Stevenson M., Volsky B., Hedenskog M., Volsky D. I. 1986; Immortalization of human T lymphocytes after transfection of Epstein–Barr virus DNA. Science 233:980–984
    [Google Scholar]
  37. Stollmann B., Fonatsch C. H., Havers W. 1985; Persistent EBV infection associated with monosomy 7 or chromosome 3 abnormality in childhood myeloprolipherative disorders. British Journal of Haematology 60:183–196
    [Google Scholar]
  38. Summers W. P., Grogan E. A., Shedd D., Robert M., Liu C. R., Miller G. 1982; Stable expression in mouse cells of nuclear neoantigen after transfer of a 34-megadalton cloned fragment of Epstein–Barr virus DNA. Proceedings of the National Academy of SciencesU.S.A 19:5688–5692
    [Google Scholar]
  39. Tosato G., Marti G. E., Yarchoan R., Heilman C. A., Wang F., Pike S. A., Korsmeyer S. J., Siminovitc K. 1986; Epstein–Barr virus immortalization of normal cells of B cell lineage with nonproductive rearranged immunoglobulin genes. Journal of Immunology 137:2037–2042
    [Google Scholar]
  40. Weis I. J., Tedder T. F., Fearon D. T. 1984; Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proceedings of the National Academy of SciencesU.S.A 81:881–885
    [Google Scholar]
  41. Zur Hausen H., O’neil F. J., Freese U. K., Hecker E. 1978; Persisting oncogenic herpes virus induced by tumour promoter TPA. Nature, London 272:373–375
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-5-1203
Loading
/content/journal/jgv/10.1099/0022-1317-70-5-1203
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error