1887

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Volume 70, Issue 5

Identification of L2 Open Reading Frame Gene Products of Bovine Papillomavirus Type 1 Using Monoclonal Antibodies Free

Abstract

Summary

Four hybridoma cell lines producing monoclonal antibodies (MAbs) to bovine papillomavirus type 1 (BPV-1) L2 open reading frame (ORF) gene products have been established from mice immunized with a BPV-1 L2--galactosidase fusion protein. Hybridomas were selected and cloned (from over 700 hybridomas) on the basis of specific reactivity of supernatant fluids with BPV-1 L2 epitopes on disrupted BPV-1 particles and L2--galactosidase fusion proteins by ELISA and Western blotting, and with acetone-fixed frozen sections of BPV-1-induced fibropapillomas by immunofluorescence. These MAbs were not reactive with intact BPV-1 particles or BPV-1 L1--galactosidase fusion proteins by ELISA or with -galactosidase by ELISA and Western blotting. The four MAbs detected viral structural proteins of 76K, 68K and possibly 55K in purified BPV-1 preparations by Western blotting. Two of the four MAbs were cross-reactive with BPV-2-induced fibropapillomas. These findings suggest that (i) the BPV-1 L2 ORF encodes the minor capsid protein(s), (ii) the gene products of the BPV-1 L2 ORF have values of 76K, 68K and possibly 55K, (iii) minor capsid epitopes are internal to the BPV-1 particle, and (iv) MAbs reactive with genetically engineered truncated BPV-1 L2 ORF gene products can distinguish between BPV-1 and BPV-2 productive infections.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BPV-1 , fusion proteins and L2 ORF
© Society for General Microbiology 1989
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1989-05-01
2024-04-20
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Identification of L2 Open Reading Frame Gene Products of Bovine Papillomavirus Type 1 Using Monoclonal Antibodies
J. Gen. Virol. 70, 1133 (1989); https://doi.org/10.1099/0022-1317-70-5-1133
/content/journal/jgv/10.1099/0022-1317-70-5-1133
/content/journal/jgv/10.1099/0022-1317-70-5-1133
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