1887

Abstract

Summary

Twenty-one monoclonal antibodies reactive with Junin virus structural proteins were produced and characterized. Using radioimmunoprecipitation and Western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glyco-protein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. In radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35K structural glycoprotein, but also with what is presumed to be the glycoprotein precursor. Four of seven glycoprotein-specific antibodies neutralized Junin virus to high titres. Cross-reactivity with other arenaviruses was found to be restricted to nucleoprotein-specific monoclonal antibodies and occurred only with New World arenaviruses. Cross-reactivity also shows the Junin virus to be most closely related to Machupo and Tacaribe viruses.

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1989-05-01
2022-01-24
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References

  1. Auperin D., Romanowski V., Galinski M., Bishop D. H. L. 1984; Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA. Journal of Virology 52:897–904
    [Google Scholar]
  2. Bishop D. H. L., Auperin D. D. 1987; Arenavirus gene structure and organization. Current Topics in Microbiology and Immunology 133:5–17
    [Google Scholar]
  3. Buchmeier M. J., Parekh B. S. 1987; Protein structure and expression among arena viruses. Current Topics in Microbiology and Immunology 133:41–57
    [Google Scholar]
  4. Buchmeier M. J., Lewicki H. A., Tomori O., Oldstone M. B. A. 1981; Monoclonal antibodies to lymphocytic choriomeningitis and Pichinde viruses: generation, characterization, and cross-reactivity with other arenaviruses. Virology 113:73–85
    [Google Scholar]
  5. Casals J., Buckley S. M., Cedeno R. 1975; Antigenic properties of the arenaviruses. Bulletin of the World Health Organization 52:421–427
    [Google Scholar]
  6. Clegg J. C. S., Lloyd G. 1983; Structural and cell-associated proteins of Lassa virus. Journal of General Virology 64:1127–1136
    [Google Scholar]
  7. Coto C. E., Damonte E. B., Calello M. A., Weissenbacher M. C. 1980; Protection of guinea pigs inoculated with Tacaribe virus against lethal doses of Junin virus. Journal of Infectious Diseases 141:389–393
    [Google Scholar]
  8. Cresta B., Padula P., De Martinez Segovia Z. M. 1980; Biological properties of Junin virus proteins. Inter-virology 13:284–288
    [Google Scholar]
  9. De Martinez Segovia Z. M., De Mitri M. I. 1977; Junin virus structural proteins. Journal of Virology 21:579–583
    [Google Scholar]
  10. De Mitri M. I., De Martinez Segovia Z. M. 1985; Polypeptide synthesis in Junin virus-infected BHK-21 cells. Ada virologica 29:97–103
    [Google Scholar]
  11. Elliott L. H., McCormick J. B., Johnson K. M. 1982; The inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. Journal of Clinical Microbiology 16:704–708
    [Google Scholar]
  12. Grau O., Franze-Fernandez M., Romanowski V., Rustici S. M., Rosas M. F. 1981; Junin virus structure. In The Replication of Negative Strand Viruses11–14 Bishop D. H. L., Compans R. W. New York: Elsevier;
    [Google Scholar]
  13. Harnish D. G., Leung W-C., Rawls W. E. 1981; Characterization of polypeptides immunoprecipitable from Pichinde virus-infected BHK-21 cells. Journal of Virology 38:840–848
    [Google Scholar]
  14. Hendry R. M., McLntosh K. 1982; Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description. Journal of Clinical Microbiology 16:324–328
    [Google Scholar]
  15. Howard C. R., Lewicki H., Allison L., Salter M., Buchmeier M. J. 1985; Properties and characterization of monoclonal antibodies to Tacaribe virus. Journal of General Virology 66:1383–1395
    [Google Scholar]
  16. Johnson K. M., Elliott L. H., Heymann D. L. 1981; Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. Journal of Clinical Microbiology 14:527–529
    [Google Scholar]
  17. Kenyon R. H., Green D. E., Eddy G. A., Peters C. J. 1986; Treatment of Junin virus-infected guinea pigs with immune serum: development of late neurological disease. Journal of Medical Virology 20:207–218
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 111:680–685
    [Google Scholar]
  19. Lukashevich I. S., Lemeshko N. N. 1985; Machupo virus polypeptides: identification by immunoprecipitation. Archives of Virology 86:85–99
    [Google Scholar]
  20. McKee K. T. jr, Mahlandt B. G., Bagley L. R., Lyerly W. H. jr, Peters C. J., Eddy G. A. 1985; Infection of Cebus monkeys with Junin virus. Medicina, Buenos Aires 45:144–152
    [Google Scholar]
  21. McKee K. T. jr, Mahlandt B. G., Maiztegui J. I., Green D. E., Peters C. J. 1987; Virus-specific factors in experimental Argentine hemorrhagic fever in rhesus macaques. Journal of Medical Virology 22:99–111
    [Google Scholar]
  22. Padula P. J., De Martinez Segovia Z. M. 1984; Replication of Junin virus in the presence of tunicamycin. Inter-virology 11:227–231
    [Google Scholar]
  23. Regnery R. L., Johnson K. M., Kiley M. P. 1980; Virion nucleic acid of Ebola virus. Journal of Virology 36:465–469
    [Google Scholar]
  24. Rowe W. P., Pugh W. E., Webb P. A., Peters C. J. 1970; Serological relationship of the Tacaribe complex of viruses to lymphocytic choriomeningitis virus. Journal of Virology 5:289–292
    [Google Scholar]
  25. Sabattini M. S., Gonzalez De Rios L. E., Diaz G., Vega V. R. 1977; Infeccion naturaly experimental de roedores con virus Junin. Medicina, Buenos Aires 37:149–161
    [Google Scholar]
  26. Sanchez A., Kiley M. P. 1987; Identification and analysis of Ebola virus messenger RNA. Virology 157:414–420
    [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:. Proceedings of the National Academy of SciencesU.S.A 76:4350–4354
    [Google Scholar]
  28. Weissenbacher M. C., Coto C. E., Calello J. A., Rondinone S. N., Damonte E. B., Frigeria J. J. 1982; Cross-protection in nonhuman primates against Argentine hemorrhagic fever. Infection and Immunity 35:425–430
    [Google Scholar]
  29. Weissenbacher M. C., Sabattini M. S., Avila M. M., Sangiorgio P. M., De Sensi M. R. F., Contigiani M. S., Del C. Levis S., Maiztegui J. L. 1983; Junin virus activity in two rural populations of the Argentine hemorrhagic fever (AHF) endemic area. Journal of Medical Virology 12:272–280
    [Google Scholar]
  30. Wulff H., Lange J. V., Webb P. A. 1978; Interrelationships among arenaviruses measured by indirect immuno- fluorescence. Intervirology 9:344–350
    [Google Scholar]
  31. Young P. R., Chanas A. C., Lee S. R., Gould E. A., Howard C. R. 1987; Localization of an arenavirus protein in the nuclei of infected cells. Journal of General Virology 68:2465–2470
    [Google Scholar]
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