The Temperature Sensitivity of the Sabin Type 3 Vaccine Strain of Poliovirus: Molecular and Structural Effects of a Mutation in the Capsid Protein VP3 Free

Abstract

Summary

The growth of the Sabin strain of type 3 poliovirus is reduced at high temperatures compared to that of its virulent precursor strain Leon. Recombinant viruses have been generated from infectious cDNA clones and demonstrate that the temperature-sensitive (ts) phenotype is mainly attributable to a difference in residue 91 of the virion protein VP3. Examination of non-ts mutants derived or reveals the existence of second site mutations some of which are clearly able to suppress the ts phenotype. The location of residue 91 of VP3, and of a number of candidate suppressor mutations, in the atomic structure of the virion suggests that the ts phenotype may result in destabilization of the particle and that the suppressors may function by stabilizing specific interfaces. It is not yet clear whether the ts phenotype is expressed at the level of the particle or in the form of defects in assembly or uncoating of the virion, or all three.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-5-1117
1989-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/5/JV0700051117.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-5-1117&mimeType=html&fmt=ahah

References

  1. Cann A. J., Stanway C., Hughes P. J., Minor P. D., Evans D. M. A., Schild G. C., Almond J. W. 1984; Reversion to virulence of the live attenuated Sabin type 3 oral poliovirus vaccine. Nucleic Acids Research 12:7787–7792
    [Google Scholar]
  2. Evans D. M. A., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V. Jr 1985; A single nucleotide change in the 5′ non-coding region of the genome of the Sabin type 3 poliovaccine is associated with increased neurovirulence. Nature, London 314:548–550
    [Google Scholar]
  3. Hogle J. M., Chow M., Filman D. J. 1985; The three-dimensional structure of poliovirus at 2.9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  4. Ming L., Vriend G., Kamer G., Minor I., Arnold E., Rossmann M. G., Boege U., Scraba D. G., Duke G. M., Palmenberg A. C. 1987; The atomic structure of mengovirus at 3.0 Å resolution. Science 235:182–190
    [Google Scholar]
  5. Minor P. D., Ferguson M., Evans D. M. A., Almond I. W., Icenogle J. P. 1986a; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  6. Minor P. D., John A., Ferguson M., Icenogle J. P. 1986b; Antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee. Journal of General Virology 67:693–706
    [Google Scholar]
  7. Nomoto A., Omata T., Toyoda H., Kuge S., Horie H., Kataoka Y., Genba Y., Nakano Y., Imura N. 1982; Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome. Proceedings of the National Academy of SciencesU.S.A 79:5793–5797
    [Google Scholar]
  8. Omata T., Kohara M., Kuge S., Komabu T., Shinabu A., Semler B. L., Kameda A., Itoh H., Arita M., Wimmer E., Nomoto A. 1986; Genetic analysis of the attenuation phenotype of poliovirus type 1. Journal of Virology 58:348–358
    [Google Scholar]
  9. Racaniello V. R., Baltimore D. 1981; Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916–919
    [Google Scholar]
  10. Sabin A. B., Boulger L. R. 1973; History of Sabin attenuated poliovirus oral live vaccine strains. Journal of Biological Standardization 1:115–118
    [Google Scholar]
  11. Salk J., Cohen H., Fillastre C., Stoeckel P., Rey J. L., Schlumberger M., Nicolas I. F., Van Steenis G., Van Wezel A. L., Triau R., Saliou P., Barry L. F., Moreau J. P., Merieux C. 1978; Killed poliovirus antigen titration in humans. Developments in Biological Standardization 41:119–132
    [Google Scholar]
  12. Smith T. J., Kremer M. S., Ming L., Vriend G., Arnold E., Kamer G., Rossmann M. G., McKinlay M. A., Diana G. D., Otto M. J. 1986; The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. Science 233:1286–1293
    [Google Scholar]
  13. Westrop G. D., Evans D. M. A., Minor P. D., Magrath D., Schild G. C., Almond I. W. 1986; Investigation of the molecular basis of attenuation in the Sabin type 3 vaccine using novel recombinant polioviruses constructed from infectious cDNA. In The Molecular Biology of Positive Strand Viruses Rowlands D. J., Mahy B. W. J., Mayo M. A. London & Orlando: Academic Press;
    [Google Scholar]
  14. World Health Organization Report 1981; Markers of poliovirus strains isolated from cases temporally associated with the use of live poliovirus vaccine: report on a WHO collaborative study. Journal of Biological Standardization 9:163–184
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-5-1117
Loading
/content/journal/jgv/10.1099/0022-1317-70-5-1117
Loading

Data & Media loading...

Most cited Most Cited RSS feed