1887

Abstract

Summary

Monoclonal antibodies (MAbs) were prepared against the cylindrical inclusion protein (CIP), amorphous inclusion protein (AIP) and capsid protein (CP) of watermelon mosaic virus 2 (WMV2). Using the MAbs, CIP, AIP and CP were detected, roughly quantitatively, in WMV2-infected pumpkin leaves showing various symptoms by using electroblot-ELISA. From symptomless leaves and most dark green areas in the mosaic pattern no protein or small amounts of the three proteins were detected, but from most yellow areas in the mosaic almost equal amounts of each protein were detected in abundance. Leaves showing mild vein-yellowing and vein-clearing (respectively, the first and second leaves of the plants tested) contained AIP and CP in large amounts, but little CIP. On the other hand, expanding leaves contained CIP and AIP in large quantities, but CP in traces only. Therefore the distributions of CIP, AIP and CP in pumpkin plants were very uneven, but correlated with symptoms. In addition, the ratio of the concentrations of CIP, AIP and CP varied from tissue to tissue.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-5-1085
1989-05-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-5-1085
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error