RT Journal Article SR Electronic(1) A1 Demler, S. A. A1 De Zoeten*, G. A.YR 1989 T1 Characterization of a Satellite RNA Associated with Pea Enation Mosaic Virus JF Journal of General Virology, VO 70 IS 5 SP 1075 OP 1084 DO https://doi.org/10.1099/0022-1317-70-5-1075 PB Microbiology Society, SN 1465-2099, AB Summary The bipartite genome of pea enation mosaic virus (PEMV) is often accompanied by a non-essential third RNA (M r 0·3 × 106) of unknown origin and function. Although the Wisconsin strains of PEMV originally lacked this RNA, we have monitored the appearance of a putative replicative form of this species in PEMV-infected tissue. In later generations encapsidated single-stranded RNA 3 appeared. We have used a 750 bp clone generated against the ds replicative form of RNA 3 to probe viral and host-derived nucleic acids to establish the relationship of this RNA to PEMV infection. Northern blot analysis showed that RNA 3 is distinct from viral genomic RNA and from host RNA. Similarly, Southern blot analysis showed that RNA 3 is distinct from the host genome. Infectivity analysis of fractionated viral RNAs coupled with Northern blot analysis confirmed that RNA 3 is both non-essential for PEMV infection, and non-infectious when inoculated on its own. RNA 3 does not influence symptom expression, aphid transmission or particle morphology. We conclude that RNA 3 of PEMV is a satellite RNA., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-5-1075