1887

Abstract

Summary

Several strains of cucumber mosaic virus (CMV) have been classified, and nucleic acid hybridization data indicate that these strains differ widely in nucleotide sequence. We have constructed cDNA clones of the coat protein coding regions of CMV strains C and WL, and have compared the nucleotide sequences of the RNA 3 intergenic region, coat protein gene, and 3′ untranslated region with published CMV sequences from the same regions of the Q, D and Y strains. These comparisons show that the C and WL strains belong to different CMV subgroups, and that the subgroups are more closely related in sequence than suggested by previous nucleic acid hybridization studies.

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1989-05-01
2024-04-25
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References

  1. Ahlquist P., Dasgupta R., Kaesberg P. 1981; Near identity of 3′ RNA secondary structure in bromoviruses and cucumber mosaic virus. Cell 23:183–189
    [Google Scholar]
  2. Bujarski J. J., Dreher T. W., Hall T. C. 1985; Deletions in the 3′-terminal tRNA-like structure of brome mosaic virus RNA differentially affect aminoacylation and replication in vitro . Proceedings of the National Academy of SciencesU.S.A 82:5636–5640
    [Google Scholar]
  3. Chang L.-Y., Slightom J. L. 1984; Isolation and nucleotide sequence analysis of the β-type globin pseudogene from human, gorilla, and chimpanzee. Journal of Molecular Biology 180:767–784
    [Google Scholar]
  4. Cuozzo M., O’Connell K., Kaniewski W., Fang R.-X., Chua N.-H., Tumer N. E. 1988; Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6:549–557
    [Google Scholar]
  5. Davies C., Symons R. H. 1988; Further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus RNA 3. Virology 165:216–224
    [Google Scholar]
  6. Dayhoff M. O., Schwarz R. M., Orcutt B. C. 1979; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 53345–352 Dayhoff M. O. Washington, D.C.: National Biomedical Research Foundation;
    [Google Scholar]
  7. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  8. Devergne J. C., Cardin L. 1973; Contribution à l’étude du virus de la mosaïque du concombre (CMV). IV. Essai de classification de plusieurs isolats sur la base de leur structure antigenique. Annals of Phytopathology 5:409–430
    [Google Scholar]
  9. Devergne J. C., Cardin L. 1975; Relations sérologiques entre cucumovirus (CMV, TAV, PSV). Annals of Phytopathology 7:255–276
    [Google Scholar]
  10. Edwards M. C., Gonsalves D. 1983; Grouping of seven biologically defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73:1117–1120
    [Google Scholar]
  11. French R., Ahlquist P. 1987; Intercistronic as well as terminal sequences are required for efficient amplification of brome mosaic virus RNA 3. Journal of Virology 61:1457–1465
    [Google Scholar]
  12. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RN A sequence homology between strains of plant viruses : its application to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  13. Gonsalves D., Provvidentl R., Edwards M. C. 1982; Tomato whiteleaf: the relation of an apparent satellite RNA and cucumber mosaic virus. Phytopathology 72:1533–1538
    [Google Scholar]
  14. Gould A. R., Symons R. H. 1982; Cucumber mosaic virus RNA 3. Determination of the nucleotide sequence provides the amino acid sequences of protein 3A and viral coat protein. European Journal of Biochemistry 126:217–227
    [Google Scholar]
  15. Haenni A.-L., Joshi S., Chapeville F. 1982; tRNA-like structures in the genomes of RNA viruses. Progress in Nucleic Acid Research and Molecular Biology 27:85–104
    [Google Scholar]
  16. Hall T. C. 1979; Transfer RNA-like structures in viral genomes. International Review of Cytology 60:1–26
    [Google Scholar]
  17. Hemenway C., Fang R.-X., Kaniewsky W. K., Chua N.-H., Tumer N. E. 1988; Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO Journal 1:1273–1280
    [Google Scholar]
  18. Hidaka S., Tsunasawa S., Yoon J.-O., Narita K., Takanami Y., Kubo S., Miura K. 1985; Messenger RNA structure participating in the initiation of synthesis of cucumber mosaic virus coat protein. Journal of Biochemistry 97:161–171
    [Google Scholar]
  19. Huynh T. V., Young R. A., Davis R. W. 1985; Constructing and screening cDNA libraries in Agt 10 and Agt 11. In DNA Cloning: A Practical Approach 149–78 Glover D. M. Oxford & Washington, D.C.: IRL Press;
    [Google Scholar]
  20. Kaper J. M., Waterworth H. E. 1981; Cucumoviruses. In Plant Virus Infections and Comparative Diagnosis257–332 Kurstak E. New York: Elsevier/North-Holland;
    [Google Scholar]
  21. Kohl R. J., Hall T. C. 1974; Aminoacylation of RNA from several viruses: amino acid specificity and differential activity of plant, yeast and bacterial synthetases. Journal of General Virology 25:257–261
    [Google Scholar]
  22. Loesch-Fries L. S., Merlo D., Zinnen T., Burhop L., Hill K., Krahn K., Jarvis N., Nelson S., Halk E. 1987; Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers resistance. EMBO Journal 6:1845–1851
    [Google Scholar]
  23. Lot H., Marrou J., Quiot J. G., Esvan C. 1972; Contribution à l’étude du virus de la mosaïque du concombre (CMV). I. Methode de purification rapide du virus. Annals of Phytopathology 4:25–38
    [Google Scholar]
  24. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Marrou J., Quiot J. B., Marchoux G. 1975; Caractérisation de douze souches du VMC par leurs aptitudes pathogenés: tentative de classification. Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 40:107–122
    [Google Scholar]
  26. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  27. Miller W. A., Bujarski J. J., Dreher T. W., Hall T. C. 1986; Minus-strand initiation by brome mosaic virus replicase within the 3′ tRNA-like structure of native and modified RNA templates. Journal of Molecular Biology 187:537–546
    [Google Scholar]
  28. Nelson R. S., Powell-Abel P., Beachy R. N. 1987; Lesions and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein gene of tobacco mosaic virus. Virology 158:126–132
    [Google Scholar]
  29. Nelson R. S., McCormick S. M., Dellanay X., Dube P., Layton J., Anderson E. J., Kaniewska M., Proksch R. K., Horsch R. B., Rogers S. G., Fraley R. T., Beachy R. N. 1988; Virus tolerance, plant growth, and field performance of transgenic tomato plants expressing coat protein from tobacco mosaic virus. Bio/Technology 6:403–109
    [Google Scholar]
  30. Piazzolla P., Diaz-Ruiz J. R., Kaper I. M. 1979; Nucleic acid homologies of eighteen cucumber mosaic virus isolates determined by competition hybridization. Journal of General Virology 45:361–369
    [Google Scholar]
  31. Polites H. G., Marotti K. R. 1986; A step-wise protocol for cDNA synthesis. Biotechniques 4:514–520
    [Google Scholar]
  32. Powell-Abel P., Nelson R. S., De B., Hoffman N., Rogers S. G., Fraley R. T., Beachy R. N. 1986; Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743
    [Google Scholar]
  33. Rezaian M. A., Williams R. H. V., Gordon K. H. J., Gould A- R., Symons R. H. 1984; Nucleotide sequence of cucumber-mosaic-virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses. European Journal of Biochemistry 143:277–284
    [Google Scholar]
  34. Rezaian M. A., Williams R. H. V., Symons R. H. 1985; Nucleotide sequence of cucumber mosaic virus RNA 1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNA’s. European Journal of Biochemistry 150:331–339
    [Google Scholar]
  35. Rietveld K., Pleij C. W. A., Bosch L. 1983; Three-dimensional models of the tRNA-like 3′ termini of some plant viral RNAs. EMBO Journal 2:1079–1085
    [Google Scholar]
  36. Rizzo T. M., Palukaitis P. 1988; Nucleotide sequence and evolutionary relationships of cucumber mosaic vims (CMV) strains: CMV RNA 2. Journal of General Virology 69:1777–1787
    [Google Scholar]
  37. Slightom J. L., Theisen T. W., Koop B. F., Goodman M. 1987; Orangutan fetal globin genes. Nucleotide sequences reveal multiple gene conversions during hominid phytogeny. Journal of Biological Chemistry 262:7472–7483
    [Google Scholar]
  38. Tumer N. E., O’Connell K. M., Nelson R. S., Sanders P. R., Beachy R. N., Fraley R. T., Shah D. M. 1987; Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants. EMBO Journal 6:1181–1188
    [Google Scholar]
  39. Van Dun C. M. P., Bol J. F., Van Vloten-Doting L. 1987; Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159:299–305
    [Google Scholar]
  40. Wiener A. M., Maizels N. 1987; tRNA-like structures tag the 3′ ends of genomic RNA molecules for replication: implications for the origin of protein synthesis. Proceedings of the National Academy of SciencesU.S.A 84:7383–7387
    [Google Scholar]
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