1887

Abstract

SUMMARY

The pattern of human papillomavirus type 16 (HPV-16) integration was studied in 23 invasive carcinomas of the cervix using subgenomic probes. Seventeen tumours contained integrated HPV-16 and in 13 of these there was evidence of disruption within the E1–E2 open reading frames (ORFs). In all cases the upstream regulatory region (URR)–E6–E7 ORFs was maintained intact. Two independently derived tumours were infected with episomal wild-type HPV-16 and an episomal variant of HPV-16 containing a 325 bp deletion within the URR (positions 7598 to 17) and a point mutation at position 20 (A to C). This is the first report of a variant HPV-16 which is likely to be both defective and transmissible. Loss of E2 expression and deletion of a large portion of the URR may be two of the mechanisms leading to altered HPV-16 early gene expression in cervical tumours.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-4-999
1989-04-01
2024-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/4/JV0700040999.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-4-999&mimeType=html&fmt=ahah

References

  1. Bankier A. T., Barrell B. G. 1983; Shotgun sequencing. Techniques in the life Sciences1–33 Flavell R. A. Limerick; Elsevier/North-Holland:
    [Google Scholar]
  2. Bedell M. A., Jones K. H., Laimins L. A. 1987; The E6–E7 region of human papillomavirus type 18 is sufficient for transformation of NIH 3T3 and Rat-1 cells. Journal of Virology 61:3635–3640
    [Google Scholar]
  3. Chin M. T., Hirochika R., Hirochika H., Broker T., Chow L. T. 1988; Regulation of human papillomavirus type 11 enhancer and E6 promoter by activating and repressing proteins from the E2 open reading frame: functional and biochemical studies. Journal of Virology 62:2994–3002
    [Google Scholar]
  4. Choo K-B., Pan C-C., Han S-H. 1987; Integration of human papillomavirus type 16 into cellular DNA of cervical carcinoma: preferential deletion of the E2 gene and invariable retention of the long control region and the E6/E7 open reading frames. Virology 161:259–261
    [Google Scholar]
  5. Cripe T. P., Haugen T. H., Turk J. P., Tabatabai F., Schmid P. G. III, Dürst M., Gissmann L., Roman A., Turek L. p. 1987; Transcriptional regulation of the human papillomavirus 16 E6–E7 promoter by a keratinocyte-dependent enhancer, and by viral E2 trans-activator and repressor gene products: implications for cervical carcinogenesis. EMBO Journal 6:3745–3753
    [Google Scholar]
  6. De Villiers E.-M., Wagner D., Schneider A., Miklaw H., Papendick U., Wagner D., Wesch H., Wahrendorf J., Zur Hausen H. 1987; Human papillomavirus infection in women with and without abnormal cervical cytology. Lancet ii:703–706
    [Google Scholar]
  7. Dostatni N., Thierry F., Yaniv M. 1988; A dimer of BPV-1 E2 containing a protease resistant core interacts with its DNA target. EMBO Journal 7:3807–3816
    [Google Scholar]
  8. Dürst M., Kleinheinz A., Hotz M., Gissmann L. 1985; The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. Journal of General Virology 66:1515–1522
    [Google Scholar]
  9. Dürst M., Croce C. M., Gissmann L., Schwarz E., Huebner K. 1987; Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas. Proceedings of the National Academy of Sciences U.S.A: 84:1070–1074
    [Google Scholar]
  10. Gloss B., Bernard H. u., Seedorf K., Klock G. 1987; The upstream regulatory region of the human papillomavirus type 16 contains an E2 protein independent enhancer which is specific for cervical carcinoma cells and regulated by glucocorticoid hormones. EMBO Journal 6:3735–3743
    [Google Scholar]
  11. Hirochika H., Hirochika R., Broker T. R., Chow L. T. 1988; Functional mapping of the human papillomavirus type 11 transcriptional enhancer and its interaction with the trans-acting E2 proteins. Genes and Development 2:54–67
    [Google Scholar]
  12. Kanda T., Watanabe S., Yoshiike K. 1988; Immortalization of primary rat cells by human papillomavirus type 16 subgenomic DNA fragments controlled by the SV40 promoter. Virology 165:321–325
    [Google Scholar]
  13. Lehn H., Villa L. L., Marziona F., Hilgarth M., Hillemans H.-G., Sauer G. 1988; Physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract. Journal of General Virology 69:187–196
    [Google Scholar]
  14. Moskaluk C. A., Bastia D. 1988; Interaction of the bovine papillomavirus type 1 E2 transcriptional control protein with the viral enhancer: purification of the DNA-binding domain and analysis of its contact points with DNA. Journal of Virology 62:1925–1931
    [Google Scholar]
  15. Murdoch I. B., Cassidy L. J., Fletcher K., Cordiner J. W., Macnab J. C. M. 1988; Histological and cytological evidence of viral infection and human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and normal tissue in the west of Scotland: evaluation of treatment policy. British Medical Journal 296:381–385
    [Google Scholar]
  16. Saiki R. K., Grelfand D. M., Stoffel S., Scharf S. J., Miguchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  17. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A: 74:5463–5467
    [Google Scholar]
  18. Schneider-Maunoury S., Croissant O., Orth G. 1987; Integration of human papillomavirus type 16 DNA sequences: a possible early event in the progression of genital tumors. Journal of Virology 61:3295–3298
    [Google Scholar]
  19. Seedorf K., Krämmer G., Dürst M., Suhai S., Röwekamp W. G. 1985; Human papillomavirus type 16 DNA sequence. Virology 145:181–185
    [Google Scholar]
  20. Shirasawa H., Tomita Y., Kubota K., Kasai T., Sekiya S., Takamizawa M., Simizu B. 1988; Transcriptional differences of the human papillomavirus type 16 genome between precancerous lesions and invasive carcinomas. Journal of Virology 62:1022–1027
    [Google Scholar]
  21. Smotkin D., Wettstein F. O. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences, U.S.A 83:4680–4684
    [Google Scholar]
  22. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  23. Storey A., Pim D., Murray A., Osborn K., Banks L., Crawford L. 1988; Comparison of the in vitro transforming activities of human papillomavirus types. EMBO Journal 7:1815–1820
    [Google Scholar]
  24. Thierry F., Yaniv M. 1987; The BPV1-E2 transacting protein can either be an activator or a repressor of HPV18 regulatory region. EMBO Journal 6:3391–3397
    [Google Scholar]
  25. Vousden K. H., Doniger J., Dipaolo J. A., Lowyx D. R. 1988; The E7 open reading frame of human papillomavirus type 16 encodes a transforming gene. Oncogene Research 3:167–175
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-4-999
Loading
/content/journal/jgv/10.1099/0022-1317-70-4-999
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error