1887

Abstract

SUMMARY

Six monoclonal antibodies (MAbs) to bovine coronavirus (BCV, Quebec isolate) E2 and E3 glycoproteins which were found previously to be neutralizing were examined for virus-neutralizing activity . Surgically ligated intestinal loops of newborn colostrum-deprived calves were virus-inoculated, mock-infected or inoculated with a mixture of virus and antibody. Of the six BCV-specific MAbs, four were found to be protective against a virulent field isolate of BCV, as indicated by a reduction in villous atrophy. These MAbs were specific to antigenic domain A and antigenic domains A1 and A2 on the E2 and E3 glycoproteins respectively. MAbs to antigenic domains B and C on the E2 and E3 glycoproteins, respectively, were not protective.

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1989-04-01
2022-07-07
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References

  1. Babiuk L. A., Sabara M., Hudson G. R. 1985; Rotavirus and coronavirus infections in animals Progress in Veterinary Microbiology and Immunology. 1:80–120
    [Google Scholar]
  2. Buchmeier M. J., Lewicki H. A., Talbot P. J., Knobler R. L. 1984; Murine hepatitis virus-4 (strain JHM)-induced neurologic disease is modulated in vivo by monoclonal antibody. Virology 132:261–270
    [Google Scholar]
  3. Carpio M., Bellamy J. E. C., Babiuk L. A. 1981; Comparative virulence of different bovine rotavirus isolates. Canadian Journal of Comparative Medicine 45:38–42
    [Google Scholar]
  4. Crouch C. F., Raybould T. J. G., Acres S. D. 1984; Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus. Journal of Clinical Microbiology 19:388–393
    [Google Scholar]
  5. Deregt D., Babiuk L. A. 1987; Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161:410–420
    [Google Scholar]
  6. Deregt D., Sabara M., Babiuk L. A. 1987; Structural proteins of bovine coronavirus and their intracellular processing. Journal of General Virology 68:2863–2877
    [Google Scholar]
  7. Hogue B. G., King B., Brian D. A. 1984; Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A59. Journal of Virology 51:384–388
    [Google Scholar]
  8. King B., Brian D. A. 1982; Bovine coronavirus structural proteins. Journal of Virology 42:700–707
    [Google Scholar]
  9. King B., Potts B. J., Brian D. A. 1985; Bovine coronavirus haemagglutinin protein. Virus Research 2:53–59
    [Google Scholar]
  10. Mebus C. A. 1978; Pathogenesis of coronaviral infection in calves. Journal of the American Veterinary Medical Association 173:631–632
    [Google Scholar]
  11. Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. 1973; Pathology of neonatal calf diarrhoea induced by a coronavirus-like agent. Veterinary Pathology 10:45–64
    [Google Scholar]
  12. Storz J., Kaluza G., Niemann H., Rott R. 1981; On enteropathogenic bovine coronavirus. Advances in Experimental Medicine and Biology 142:171–180
    [Google Scholar]
  13. Talbot P. J., Salmi A. A., Knobler R. L., Buchmeier M. I. 1984; Topographical mapping of epitopes on the glycoproteins of murine hepatitis virus-4 (strain JHM): correlation with biological activities. Virology 132:250–260
    [Google Scholar]
  14. Wege H., Dörries R., Wege H. 1984; Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2). Journal of General Virology 65:1931–1942
    [Google Scholar]
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