1887

Abstract

SUMMARY

Reactions of virions of the potyvirus wheat streak mosaic virus (WSMV) with homologous antiserum in leaf-dips depended on the age of infection of the leaf. Virions from young and recently expanded leaves were completely or partially covered with immunoglobulin whereas virions from leaves infected earlier were not decorated. However, in ultrathin sections virions could be immunolabelled in leaves of all ages. These results suggest that the virions can be partially degraded upon extraction, particularly those from older leaves. Thus, a negative reaction with leaf-dip serology of a suspected WSMV infection does not in itself constitute evidence that the plant is not infected with WSMV. The same principle has implications for the routine serological identification of potyviruses in leaf-dips.

Keyword(s): leaf-dip , ultrathin section , WSMV and WSSMV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-4-969
1989-04-01
2023-06-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/4/JV0700040969.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-4-969&mimeType=html&fmt=ahah

References

  1. American type culture collection; 1980 Catalogue of Strains 1, 14. Rockville; Maryland:
    [Google Scholar]
  2. Ball E. M., Brakke M. K. 1968; Leaf-dip serology for electron microscopic identification of plant viruses. Virology 36:152–155
    [Google Scholar]
  3. Brakke M. K. 1971; Wheat streak mosaic virus. CMI/AAB Descriptions of Plant Viruses48
    [Google Scholar]
  4. Brakke M. K., Ball E. M. 1968; Purification and antigenicity of wheat streak mosaic virus. Phytopathology 58:963–971
    [Google Scholar]
  5. Brakke M. K., Ball E. M., Hsu Y. H., Langenberg W. G. 1987; Wheat streak mosaic virus cylindrical inclusion body protein. Journal of General Virology 68:281–287
    [Google Scholar]
  6. Hiebert E., Mcdonald J. G. 1976; Capsid protein heterogeneity in turnip mosaic virus. Virology 70:144–150
    [Google Scholar]
  7. Lane L. C., Skopp R. 1983; The coat protein of wheat streak mosaic virus. Phytopathology 73:791
    [Google Scholar]
  8. Langenberg W. G. 1986; Virus protein association with cylindrical inclusions of two viruses that infect wheat. Journal of General Virology 67:1161–1168
    [Google Scholar]
  9. Lin N.-S. 1984; Gold-IgG complexes improve the detection and identification of viruses in leaf dip preparations. Journal of Virological Methods 8:181–190
    [Google Scholar]
  10. Lin N.-S., Langenberg W. G. 1983; Immunohistochemical localization of barley stripe mosaic virus in infected wheat cells. Journal of infrastructure Research 84:16–23
    [Google Scholar]
  11. Sabatini D. S., Bensch K., Barrnett R. I. 1963; The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17:19–58
    [Google Scholar]
  12. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  13. Shukla D. D., Strike P. M., Tracy S. L., Gough K. K., Ward C. W. 1988; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  14. Slykhuis J. T. 1973; Agropyron mosaic virus. CMI/AAB Descriptions of Plant Viruses118
    [Google Scholar]
  15. Slykhuis J. T. 1976; Wheat spindle streak mosaic virus. CMI/AAB Descriptions of Plant Viruses16
    [Google Scholar]
  16. Slykhuis J. T., Bell W. 1966; Differentiation of Agropyron mosaic, wheat streak mosaic and a hitherto unrecognized Hordeum mosaic virus in Canada. Canadian Journal of Botany 44:1191–1208
    [Google Scholar]
  17. Stein A., Salomon R., Cohen J., Loebenstein G. 1986; Detection and characterization of bean yellow mosaic virus in corms of Gladiolus grandiflorus. Annals of Applied Biology 109:147–154
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-4-969
Loading
/content/journal/jgv/10.1099/0022-1317-70-4-969
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error