1887

Abstract

SUMMARY

A near full-length cDNA clone of tobacco rattle virus (TRV) RNA-1 was constructed by joining together nine overlapping cDNA clones using restriction sites in the regions of overlap. At the 5′ end of the cDNA, oligonucleotide mutagenesis was used to insert nucleotides which were missing from the cDNA placing the construct immediately on the 3′ side of the P promoter of phage to create pTR7116. Extraneous non-viral nucleotides had been deleted from the 3′ end of the TRV cDNA to create a unique I site in pTR7116 in which the nucleotides CCC were provided by the viral cDNA, and GGG by the vector. As a result, pTR7116 could be linearized with I and transcribed to yield RNA molecules with 5′ and 3′ termini identical to those of natural TRV RNA-1. These transcripts were infectious when inoculated onto leaves of tobacco and produced the subgenomic RNA species typical of an infection with TRV RNA-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-4-963
1989-04-01
2022-12-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/4/JV0700040963.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-4-963&mimeType=html&fmt=ahah

References

  1. Abouhaidar M. A., Hirth L. 1977; 5′-terminal structure of tobacco rattle virus RNA: evidence for polarity of reconstitution. Virology 16:173–185
    [Google Scholar]
  2. Ahlquist P., Janda M. 1984; cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Molecular and Cellular Biology 4:2876–2882
    [Google Scholar]
  3. Baulcombe D. C., Buffard D. 1983; Gibberellic-acid-regulated expression of α-amylase and six other genes in wheat aleurone layers. Planta 157:493–501
    [Google Scholar]
  4. Boccara M., Hamilton W. D. O., Baulcombe D. C. 1986; The organization on interviral homologies of genes at the 3′ end of tobacco rattle virus RNA-1. EMBO Journal 5:223–229
    [Google Scholar]
  5. Cadman C. H., Harrison B. D. 1959; Studies on the properties of soil-borne viruses of tobacco-rattle type occurring in Scotland. Annals of Applied Biology 47:542–556
    [Google Scholar]
  6. Deom C. M., Oliver M. J., Beachy R. N. 1987; The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237:389–394
    [Google Scholar]
  7. Goldbach R. W. 1986; Molecular evolution of plant RNA viruses. Annual Review of Phytopathology 24:289–310
    [Google Scholar]
  8. Hamilton W. D. O., Boccara M., Robinson D. J., Baulcombe D. C. 1987; The complete nucleotide sequence of tobacco rattle virus RNA-1. Journal of General Virology 68:2563–2575
    [Google Scholar]
  9. Harrison B. D., Robinson D. J. 1978; The tobraviruses. Advances in Virus Research 23:25–77
    [Google Scholar]
  10. Harrison B. D., Robinson D. J. 1981; Tobraviruses. Handbook of Plant Virus Infections and Comparative Diagnosis515–540 Kurstak E. Amsterdam: Elsevier;
    [Google Scholar]
  11. Harrison B. D., Robinson D. J. 1986; Tobraviruses. In The Plant Viruses 2339–369 van Regenmortel M. H. V., Fraenkel-Conrat H. New York: Plenum Press;
    [Google Scholar]
  12. Knorr D. A., Hawson W. O. 1988; A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity in Nicotiana sylvestris . Proceedings of the National Academy of Sciences U.S.A 85:170–174
    [Google Scholar]
  13. Lister R. M., Hadidi A. F. 1971; Some properties of apple chlorotic leaf spot virus and their relation to purification problems. Virology 45:204–251
    [Google Scholar]
  14. Meshi T., Watanabe Y., Saito T., Sugimoto A., Maeda T., Okada y. 1987; Function of the 30 Kd protein of tobacco mosaic virus; involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
    [Google Scholar]
  15. Meshi T., Motoyoshi F., Adachl A., Watanabe Y., Takamatsu N., Okada Y. 1988; Two concomitant base substitutions in the putative replicase genes of tobacco mosaic virus confer the ability to overcome the effects of a tomato resistance gene, Tm-1. EMBO Journal 7:1575–1581
    [Google Scholar]
  16. Minson A. C., Darby G. 1973; 3′ terminal oligonucleotide fragments of tobacco rattle virus ribonucleic acids. Journal of Molecular Biology 77:337–340
    [Google Scholar]
  17. Ohno T., Takamatsu N., Meshi T., Okada Y., Nishiguchi M., Kiho Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
    [Google Scholar]
  18. Robinson D. J., Mayo M. A., Fritsch C., Jones A. T., Raschké J. H. 1983; Origin and messenger activity of two small RNA species found in particles of tobacco rattle virus strain SYM. Journal of General Virology 64:1591–1599
    [Google Scholar]
  19. Saito T., Meshi T., Takamatsu N., Okada Y. 1987; Coat protein gene sequence of tobacco mosaic virus encodes a host response determinant. Proceedings of the National Academy of Sciences U.S.A 84:6074–6077
    [Google Scholar]
  20. van Belkum A., Cornelissen B., Linthorst H., Bol J., Pleijy C., Bosch L. 1987; tRNA-like properties of tobacco rattle virus RNA. Nucleic Acids Research 15:2837–2850
    [Google Scholar]
  21. Zimmern D., Hunter T. 1983; Point mutation in the 30-k open reading frame of TMV implicated in temperature-sensitive assembly and local lesion spreading of mutant N :2519. EMBO Journal 2:1893–1900
    [Google Scholar]
  22. Zoller M. J., Smith M. 1987; Oligonucleotide-directed mutagenesis : a simple method using two oligonucleotide primers and a single-stranded DNA template. Methods in Enzymology 154:329–350
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-4-963
Loading
/content/journal/jgv/10.1099/0022-1317-70-4-963
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error