Full text loading...
Abstract
Chicory yellow mottle virus (CYMV) is a nepovirus isolated from chicory in southern Italy. The strain CYMV-T, besides the genomic RNAs, contains additional encapsidated RNA components, the most prominent of which have estimated M r values of 500000 (500K) and 170000 (170K). Northern blot hybridization experiments confirmed that the 170K molecule is a satellite RNA and indicated that the 500K RNA is neither a subgenomic RNA nor a trimeric form of the 170K RNA, but is another satellite RNA. The two satellite RNAs also differ significantly at their termini. The 170K RNA has a free hydroxyl group at the 5′ terminus, where the 500K RNA is blocked. At the 3′ termini, the 170K RNA has a cyclic 2′,3′-phosphate whereas the 500K RNA has a poly(A) tail. Thus CYMV-T seems capable of supporting two satellites with structural characteristics that resemble two types of satellite previously found in association only with two different nepoviruses.
- Received:
- Accepted:
- Published Online: