1887

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Volume 70, Issue 4

Characterization of IgG Fc Receptors Induced by Human Cytomegalovirus Free

Abstract

SUMMARY

Immunoglobulin G Fc receptors (IgG FcRs) induced by human cytomegalovirus (HCMV) were isolated from solubilized HCMV-infected cell extracts by IgG affinity chromatography and analysed by Western blotting using I-labelled human IgG and Fc. FcR species of 130K, 65K, 50K and 38K were found to mediate the binding of IgG. The 130K and 65K FcRs were also detected on HCMV virions. All four FcRs were reactive with a murine monoclonal antibody to herpes simplex virus glycoprotein E. Anti-HCMV antibodies markedly inhibited the binding of I-labelled human IgG Fc to the 130K and 50K species but inhibited to a lesser extent the binding to the 65K FcR.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cytomegalovirus , Fc receptors , human and IgG
© Society for General Microbiology 1989
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1989-04-01
2024-04-23
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References

  1. Adler R., Glorioso J. C., Cossman J., Levine M. 1978; Possible role of Fc receptor on cells infected and transformed by herpesvirus: escape from immune cytolysis.. Infection and Immunity 21:442–447
     [Google Scholar]
  2. Anderson C. L., Abraham G. N. 1980; Characterization of Fc receptor for IgG on a human macrophage cell line, U937.. Journal of Immunology 125:2735–2741
     [Google Scholar]
  3. Balachandran N., Oba D. E., Hutt-Fletcher L. M. 1987; Antigenic cross-reactions among herpes simplex virus type 1 and 2, Epstein-Barr virus, and cytomegalovirus.. Journal of Virology 61:1125–1135
     [Google Scholar]
  4. Baucke R. B., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein.. Journal of Virology 32:779–789
     [Google Scholar]
  5. Byrt D., Ada G. L. 1969; An in vitro reaction between labelled flagellin or hemocyanin and lymphocyte-like cells from normal animals.. Immunology 17:503–516
     [Google Scholar]
  6. Dowler K. W., Veltri R. W. 1984; In vitro neutralization of HSV-2: inhibition by binding of normal IgG and purihed Fc to virion Fc receptor (FcR).. Journal of Medical Virology 13:251–259
     [Google Scholar]
  7. Frey J., Einsfelder B. 1984; Induction of IgG receptors in cytomegalovirus-infected human fibroblasts.. European Journal of Biochemistry 138:213–216
     [Google Scholar]
  8. Furukawa T., Fioretti A., Plotkin S. A. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication.. Journal of Virology 11:991–997
     [Google Scholar]
  9. Furukawa T., Hornberger E., Sakuma S., Plotkin S. A. 1975; Demonstration of immunoglobulin G receptors induced by human cytomegalovirus.. Journal of Clinical Microbiology 2:332–336
     [Google Scholar]
  10. Furukawa T., Gonczol E., Starr S., Tolpin M. D., Arbeter A., Plotkin S. A. 1984; HCMV envelope antigens induce both humoral and cellular immunity in guinea pigs.. Proceedings of the Society for Experimental Biology and Medicine 175:243–250
     [Google Scholar]
  11. Grundy J. E., Mckeating J. A., Griffiths P. D. 1987; Cytomegalovirus strain AD169 binds β2 microglobulin in vitro after release from cells.. Journal of General Virology 68:777–784
     [Google Scholar]
  12. Hibbs M. L., Walker I. D., Kirszbaum L., Pietersz G. A., Deacon N. J., Chambers G. W., Mckenzie I. F. C., Hogarth P. M. 1986; The murine Fc receptor for immunoglobulin: purification, partial amino acid sequence, and isolation of cDNA clones.. Proceedings of the National Academy of Sciences, U. S. A. 83:6980–6984
     [Google Scholar]
  13. Iacobs J. P., Jones C. M., Belle J. P. 1970; Characteristics of a diploid cell designated MRC-5.. Nature, London 227:168–173
     [Google Scholar]
  14. Keller R., Peitchel R., Goldman I. N., Goldman M. 1976; An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts.. Journal of Immunology 116:772–777
     [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
     [Google Scholar]
  16. Langone J. J. 1982; Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.. Advances in Immunology 32:157–252
     [Google Scholar]
  17. Lewis V. A., Koch T., Plutne H., Mellman I. 1986; A complementary DNA clone for a macrophage-lymphocyte Fc receptor.. Nature, London 324:372–375
     [Google Scholar]
  18. Mctaggart S. P., Burns W. H., White D. O., Jackson D. C. 1978; Fc receptors induced by herpes simplex virus. I. Biologic and biochemical properties.. Journal of Immunology 121:726–730
     [Google Scholar]
  19. Murayama T., Natsuume-Sakai S., Shimokawa K., Furukawa T. 1986; Fc receptor(s) induced by human cytomegalovirus bind differentially with human immunoglobulin G subclasses.. Journal of General Virology 67:1475–1478
     [Google Scholar]
  20. Para M. F., Baucke R. B., Spear P. G. 1980; Immunoglobulin G (Fc)-binding receptors on virus of herpes simplex virus type 1 and transfer of these receptors to the cell surface by infection.. Journal of Virology 34:512–520
     [Google Scholar]
  21. Para M. F., Goldstein L., Spear P. G. 1982; Similarities and differences in the Fc-binding glycoprotein (gE) of herpes simplex virus type 1 and 2 and tentative mapping of viral gene for this glycoprotein.. Journal of Virology 41:137–144
     [Google Scholar]
  22. Peterson P., Cunningham B., Berggard I., Edelman G. 1972; β2-microglobulin – a free immunoglobulin domain.. Proceedings of the National Academy of Sciences, U.S.A. 69:1697–1701
     [Google Scholar]
  23. Rahman A. A., Teschner M., Sethi K. K., Brandis H. 1976; Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus.. Journal of Immunology 117:253–258
     [Google Scholar]
  24. Sakuma S., Furukawa T., Plotkin S. A. 1977; The characterization of IgG receptors induced by human cytomegalovirus.. Proceedings of the Society for Experimental Biology and Medicine 155:168–172
     [Google Scholar]
  25. Smithies O., Poulik M. 1972; Initiation of protein synthesis at an unusual position in an immunoglobulin gene.. Science 175:187–189
     [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proceedings of the National Academy of Sciences, U.S.A. 76:4350–4354
     [Google Scholar]
  27. Unkeless J. C., Fleit H., Mellman I. S. 1981; Structural aspects and heterogeneity of immunoglobulin Fc receptors.. Advances in Immunology 31:247–270
     [Google Scholar]
  28. Vaughn M., Taylor M., Mohanakumar T. 1985; Characterization of human IgG Fc receptors.. loumal of Immunology 135:4059–4065
     [Google Scholar]
  29. Watkins J. F. 1964; Adsorption of sensitized sheep erythrocytes of HeLa cells infected with herpes simplex virus.. Nature, London 202:1364–1365
     [Google Scholar]
  30. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin.. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
     [Google Scholar]
  31. Westmoreland D., Watkins J. F. 1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG.. Journal of General Virology 24:167–178
     [Google Scholar]
  32. Westmoreland D., Stjeor S., Rapp F. 1976; The development by cytomegalovirus-infected cells of binding affinity for normal human immunoglobulin.. Journal of Immunology 116:1566–1570
     [Google Scholar]
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Characterization of IgG Fc Receptors Induced by Human Cytomegalovirus
J. Gen. Virol. 70, 893 (1989); https://doi.org/10.1099/0022-1317-70-4-893
/content/journal/jgv/10.1099/0022-1317-70-4-893
/content/journal/jgv/10.1099/0022-1317-70-4-893
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