1887

Abstract

SUMMARY

Genetic variation between clones selected from early passage pools of Wallal virus (Reoviridae, ) was investigated. The virus had been isolated in 1970 from a pool of 100 insects (), caught in the wild, by laboratory passage in suckling mice. Gel electrophoresis and oligonucleotide fingerprint analysis of clones indicated that multiple reassortant genotypes were present in early passages of the original isolate. The virus, previously described as the prototype strain of Wallal virus, was one of the reassortant clones. A virus recovered during reisolation from the same insect pool was genotypically and serologically distinct from the prototype strain and represents a second Wallal serotype. We have concluded that the clonal variation was due to the presence of two orbivirus serotypes in the original insect pool and that a range of reassortant viruses were generated during early passages of the material in mice.

Keyword(s): reassortant , serotype and Wallal virus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-4-1011
1989-04-01
2023-02-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/4/JV0700041011.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-4-1011&mimeType=html&fmt=ahah

References

  1. Doherty R. L., Carley J. G., Mackerras M. J., Marks E. N. 1963; Studies of arthropod-borne virus infections in Queensland. III. Isolation and characterisation of virus strains from wild-caught mosquitoes in north Queensland. Australian Journal of Experimental Biology and Medical Science 41:17–40
    [Google Scholar]
  2. Doherty R. L., Carley J. G., Standfast H. A., Dyce A. L., Kay B. H., Snowdon W. A. 1973; Isolation of arboviruses from mosquitoes, biting midges, sandflies and vertebrates collected in Queensland, 1969 and 1970. Transactions of the Royal Society of Tropical Medicine and Hygiene 67:536–543
    [Google Scholar]
  3. Doherty R. L., Standfast H. A., Dyce A. L., Carley J. G., Gorman B. M., Filippich C., Kay B. H. 1978; Mudjinbarry virus, an orbivirus related to Wallal virus isolated from midges from the Northern Territory of Australia. Australian Journal of Biological Sciences 31:97–103
    [Google Scholar]
  4. Gonzalez H. A., Knudson D. L. 1988; Intra- and inter-serogroup genetic relatedness of obiviruses. I. Blot hybridization of viruses of Australian serogroups. Journal of General Virology 69:125–134
    [Google Scholar]
  5. Gorman B. M. 1983; On the evolution of orbiviruses. Intervirology 20:169–180
    [Google Scholar]
  6. Gorman B. M., Leer J. R., Filippich C., Goss P. D., Doherty R. L. 1975; Plaquing and neutralization of arboviruses in the PS-EK line of cells. Australian Journal of Medical Technology 6:65–71
    [Google Scholar]
  7. Gorman B. M., Taylor J., Brown K., Melzer A. J. 1977; Analysis of ribonucleic acid of orbiviruses. Annual Report of the Queensland Institute of Medical Research 31:15
    [Google Scholar]
  8. Gorman B. M., Taylor J., Walker P. J., Young P. R. 1978; The isolation of recombinants between related orbiviruses. Journal of General Virology 41:333–342
    [Google Scholar]
  9. Gorman B. M., Taylor J., Walker P. J. 1983; Orbiviruses. The Reoviridae287–357 Joklik W. K. New York & London: Plenum Press;
    [Google Scholar]
  10. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1987; Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology 157:172–179
    [Google Scholar]
  11. Kahlon J., Sugiyama K., Roy P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  12. Karabatsos N. 1985 International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates, 3.1083–1084 San Antonio: The American Society of Tropical Medicine and Hygiene;
    [Google Scholar]
  13. Mecham J. O., Dean V. C., Jochim M. M. 1986; Correlation of serotype specificity and protein structure of the five U.S. serotypes of bluetongue virus. Journal of General Virology 67:2617–2624
    [Google Scholar]
  14. Mertens P. P. C., Pedley S., Cowley J. A., Gorman B. M., Jeggo M. H., Jennings D. M. 1986; Analysis of the serotype-specific nature of bluetongue virus genome segments 2 and 5 and the proteins they encode. Orbiviruses and Birnaviruses - Proceedings of the Double-Stranded RNA Virus Meeting Oxford;74–93 Roy P., Osburn B.
    [Google Scholar]
  15. Walker P. J., Mansbridge J. N., Gorman B. M. 1980; Genetic analysis of orbiviruses by using RNase T1 oligonucleotide fingerprints. Journal of Virology 34:583–591
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-4-1011
Loading
/content/journal/jgv/10.1099/0022-1317-70-4-1011
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error