%0 Journal Article %A Dore, Jean-Michel %A Pinck, Monique %A Pinck, Lothaire %T Competitive Multiplication of RNA3 Species of Different Strains of Alfalfa Mosaic Virus %D 1989 %J Journal of General Virology, %V 70 %N 3 %P 777-782 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-70-3-777 %K AlMV %K replication %K oligonucleotide priming %I Microbiology Society, %X SUMMARY Competition between RNA3 from alfalfa mosaic virus (A1MV) strain S (RNA3-S), strain B (RNA3-B) and strain 425L (RNA3-L) was studied. The identification of the RNA3 species multiplying in infected leaves was possible since the RNA3 5′ non-coding leader sequences in strains S, B and 425L differ in length. RNA3 present in total RNA from infected tobacco leaves was detected, and strains were identified from the length of the cDNA reverse-transcribed from RNA primed with a specific oligonucleotide. In competition experiments the inoculum, containing known amounts of RNA1, 2, 3 and 4 of one strain, was complemented with various amounts of heterologous RNA3 and inoculated to a systemic host. It is shown that RNA3-S was better replicated in vivo by the A1MV replicase of strain B than was RNA3-B itself, and to a lesser extent better replicated by the A1MV replicase of strain L than was RNA3-L. Comparison of genetic information carried by the RNA3 species present in the inoculum suggests that the more efficient multiplication of RNA3-S is related to the structure of the leader sequence of RNA3-S. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-3-777