1887

Abstract

SUMMARY

Competition between RNA3 from alfalfa mosaic virus (A1MV) strain S (RNA3-S), strain B (RNA3-B) and strain 425L (RNA3-L) was studied. The identification of the RNA3 species multiplying in infected leaves was possible since the RNA3 5′ non-coding leader sequences in strains S, B and 425L differ in length. RNA3 present in total RNA from infected tobacco leaves was detected, and strains were identified from the length of the cDNA reverse-transcribed from RNA primed with a specific oligonucleotide. In competition experiments the inoculum, containing known amounts of RNA1, 2, 3 and 4 of one strain, was complemented with various amounts of heterologous RNA3 and inoculated to a systemic host. It is shown that RNA3-S was better replicated by the A1MV replicase of strain B than was RNA3-B itself, and to a lesser extent better replicated by the A1MV replicase of strain L than was RNA3-L. Comparison of genetic information carried by the RNA3 species present in the inoculum suggests that the more efficient multiplication of RNA3-S is related to the structure of the leader sequence of RNA3-S.

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/content/journal/jgv/10.1099/0022-1317-70-3-777
1989-03-01
2022-01-24
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References

  1. Ansorge W., Labeit S. 1984; Field gradients improve resolution on DNA sequencing gels. Journal of Biochemical and Biophysical Methods 10:237–243
    [Google Scholar]
  2. Bol J. F., Van Vloten-Doting L., Jaspars E. M. J. 1971; A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85
    [Google Scholar]
  3. Dore J.-M., Pinck L. 1988; Plasmid DNA containing a copy of RNA3 can substitute for RNA3 in alfalfa mosaic virus RNA inocula. Journal of General Virology 69:1331–1338
    [Google Scholar]
  4. Godefroy-Colburn T., Ravelonandro M., Pinck L. 1985; Cap accessibility correlates with the initiation efficiency of alfalfa mosaic virus RNAs. European Journal of Biochemistry 147:549–552
    [Google Scholar]
  5. Godefroy-Colburn T., Gagey M.-J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  6. Jackson A. O., Larkins B. A. 1976; Influence of ionic strength, pH, and chelation of divalent metals on isolation of polyribosomes from tobacco leaves. Plant Physiology 57:5–10
    [Google Scholar]
  7. Langereis K., Mugnier M. A., Cornelissen B. J. C., Pinck L., Bol I. F. 1986; Variable repeats and poly(A)-stretches in the leader sequence of alfalfa mosaic virus RNA3. Virology 154:409–414
    [Google Scholar]
  8. Nassuth A., Bol J. F. 1983; Altered balance of the synthesis of plus and minus strand RNAs induced by RNA1 and 2 of alfalfa mosaic virus in absence of RNA3. Virology 124:75–85
    [Google Scholar]
  9. Nassuth A., Alblas F., Bol J. F. 1981; Localization of genetic information involved in the replication of alfalfa mosaic virus. Journal of General Virology 53:207–214
    [Google Scholar]
  10. Pinck L., Fuchs M., Pinck M., Ravelonandro M., Walter B. 1988; A satellite RNA in grapevine fanleaf virus strain F13. Journal of General Virology 69:233–239
    [Google Scholar]
  11. Qu L. H., Michot B., Bachellerie J. P. 1983; Improved methods for structure probing in large RNAs: a rapid ‘heterologous’ sequencing approach is coupled to direct mapping of nuclease accessible sites. Nucleic Acids Research 11:5903–5920
    [Google Scholar]
  12. Ravelonandro M., Godefroy-Colburn T., Pinck L. 1983; Structure of the 5′-terminal region of the genomic RNAs from two strains of alfalfa mosaic virus. Nucleic Acids Research 11:2815–2826
    [Google Scholar]
  13. Ravelonandro M., Pinck M., Pinck L. 1984; Complete nucleotide sequence of RNA3 from alfalfa mosaic virus, strain S. Biochimie 66:395–402
    [Google Scholar]
  14. Walter B., Kuszala J., Ravelonandro M., Pinck L. 1985; Alfalfa mosaic virus isolated from Buddleia davidii compared with other strains. Plant Disease 69:266–267
    [Google Scholar]
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