1887

Abstract

SUMMARY

The nucleotide sequence of the polyhedrin gene of multiple nucleocapsid polyhedrosis virus (PfMNPV) has been determined. The coding sequences of this gene shared 82% similarity at the DNA level and 88% similarity at the protein level with the polyhedrin gene from (Ac) MNPV. A single nucleotide deviation from the consensus transcription initiation sequence for baculovirus very late genes was identified in the PfMNPV polyhedrin gene. RNA was prepared from larvae infected with PfMNPV and compared with RNA harvested at 24 h post-infection from AcMNPV-infected cells using Northern blotting with an AcMNPV polyhedrin gene-specific probe. The PfMNPV mRNA was estimated to be 1·0 kb compared with a larger size of 1·15 kb for the AcMNPV polyhedrin mRNA. A cDNA copy of the 5′ end of the PfMNPV polyhedrin mRNA was made using the technique of primer extension and sequenced to demonstrate that the point of transcription initiation was similar to that of AcMNPV polyhedrin mRNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-769
1989-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/3/JV0700030769.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-3-769&mimeType=html&fmt=ahah

References

  1. Akiyoshi D., Chakerian R., Rohrmann G. F., Nesson M. H., Beaudreau G. S. 1985; Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus. Virology 141:328–332
    [Google Scholar]
  2. Birnstiel M. L., Busslinger M., Strub K. 1985; Transcription termination and 3′ processing: the end is in site!. Cell 41:349–359
    [Google Scholar]
  3. Cameron I. R., Possee R. D. 1989; Conservation of polyhedrin gene promoter function between Autographa californica and Mamestra brassicae nuclear polyhedrosis viruses. Virus Research (in press)
    [Google Scholar]
  4. Chakerian R., Rohrmann G. F., Nesson M. H., Leisy D. J., Beaudreau G. S. 1985; The nucleotide sequence of the Pieris brassicae granulosis virus granulin gene. Journal of General Virology 66:1263–1269
    [Google Scholar]
  5. Guarino L. A., Summers M. D. 1986; Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene. Journal of Virology 57:563–571
    [Google Scholar]
  6. Hooft Van Iddekinge B. J., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  7. Howard S. C., Ayres M. D., Possee R. D. 1986; Mapping the 5′ and 3′ ends of Autographa californica nuclear polyhedrosis virus polyhedrin mRNA. Virus Research 5:109–119
    [Google Scholar]
  8. Iatrou K., Ito K., Witkiewicz H. 1985; Polyhedrin gene otBombyx mori nuclear polyhedrosis virus. Journal of Virology 54:436–445
    [Google Scholar]
  9. Kuzio J., Rohel D. Z., Curry C. J., Krebs A., Carstens E. B., Faulkner P. 1984; Nucleotide sequence of the p10 polypeptide gene of Autographa californica nuclear polyhedrosis virus. Virology 139:414–418
    [Google Scholar]
  10. Leisy D., Rohrmann G., Beaudreau G. 1986a; The nucleotide sequence of the polyhedrin gene region from the multicapsid baculovirus of Orgyia pseudotsugata . Virology 153:280–288
    [Google Scholar]
  11. Leisy D., Nesson M., Pearson M., Rohrmann G., Beaudreau G. 1986b; Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 67:1073–1079
    [Google Scholar]
  12. Leisy D. J., Rohrmann G. F., Nesson M., Beaudreau G. S. 1986c; Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene. Virology 153:157–167
    [Google Scholar]
  13. Lübbert H., Doerfler W. 1984; Transcription of overlapping sets of RNAs from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs. Journal of Virology 52:255–265
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  16. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  17. Possee R. D. 1986; Cell-surface expression of the influenza virus haemagglutinin gene using a baculovirus vector. Virus Research 5:43–59
    [Google Scholar]
  18. Possee R. D., Howard S. C. 1987; Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus. Nucleic Acids Research 15:10233–10248
    [Google Scholar]
  19. Possee R. D., Kelly D. C. 1988; Physical maps and comparative DNA hybridization of Mamestra brassicae and Panolis flammea nuclear polyhedrosis virus genomes. Journal of General Virology 69:1285–1298
    [Google Scholar]
  20. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  21. Rohrmann G. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  22. Smith G. E., Summers M. D. 1982; DNA homology among subgroup A, B and C baculoviruses. Virology 123:393–406
    [Google Scholar]
  23. Smith G. E., Vlak J. M., Summers M. D. 1983; Physical analysis of Autographa californica nuclear polyhedrosis virus transcripts for polyhedrin and 10,000-molecular-weight protein. Journal of Virology 45:215–225
    [Google Scholar]
  24. Van Der Wilk F., Van Lent J. W. M., Vlak J. M. 1987; Immunogold detection of polyhedrin, p10 and virion antigens in Autographa californica nuclear polyhedrosis virus-infected Spodoptera frugiperda cells. Journal of General Virology 68:2615–2623
    [Google Scholar]
  25. Vlak J. M., Klinkenberg F. A., Zaal K. J. M., Usmany M., Klinge-Roode E. C., Geervliet I. B. F., Roosien J., Van Lent J. W. M. 1988; Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10–β-galactosidase fusion gene. Journal of General Virology 69:765–776
    [Google Scholar]
  26. Weyer U., Possee R. D. 1988; Functional analyses of the p10 gene promoter of the Autographa califomica nuclear polyhedrosis virus. Nucleic Acids Research 16:3635–3653
    [Google Scholar]
  27. Wilson M. E., Mainprize T. H., Friesen P. D., Miller L. K. 1987; Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide. Journal of Virology 61:661–666
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-769
Loading
/content/journal/jgv/10.1099/0022-1317-70-3-769
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error