1887

Abstract

SUMMARY

The relatively cell impermeable hygromycin B was found to inhibit viral but not cellular protein synthesis when added to cultures of murine hepatitis virus (MHV)-infected or mock-infected mouse L-2 fibroblasts. Membrane permeability, as judged by influx of sodium ions, has previously been demonstrated to be an MHV E2 glycoprotein-mediated, cytopathic effect of MHV infection in L-2 cells. It is therefore likely that the selective effect of hygromycin B on viral protein synthesis is a reflection of an increased drug penetration into virus-infected cells. Using hygromycin B as a marker for MHV-induced cell membrane cytopathology, the effects of drug treatment on a persistent MHV infection in mouse LM-K fibroblasts were investigated. MHV persistence in LM-K cells, which normally involves a steady state infection of 0·1 to 1 % of the cells in culture, was found to be cured by hygromycin B treatment, as measured by the elimination of infectious virus from the supernatant medium. Hygromycin B also resulted in the eradication of MHV-specific RNA from LM-K cells, arguing against the presence of a non-cytopathically or latently infected subpopulation of cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-763
1989-03-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/3/JV0700030763.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-3-763&mimeType=html&fmt=ahah

References

  1. Bailey O. T., Pappenheimer A. M., Cheever F. S., Daniels J. B. 1949; A murine hepatitis virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. Journal of Experimental Medicine 90:195–231
    [Google Scholar]
  2. Benedetto A., Rossi G. B., Amici C, Belardelli F., Cioe L., Carruba G., Carrasco L. 1980; Inhibition of animal virus production by means of translation inhibitors unable to penetrate normal cells. Virology 106:123–132
    [Google Scholar]
  3. Cameron J. M., Clemens M. I., Gray M. A., Menzies D. E., Mills B. J., Warren A. P., Pasternak C. A. 1986; Increased sensitivity of virus-infected cells to inhibitors of protein synthesis does not correlate with changes in plasma membrane permeability. Virology 155:534–544
    [Google Scholar]
  4. Cheley S., Anderson R. 1984; A reproducible microanalytical method for the detection of specific RNA sequences by dot-blot hybridization. Analytical Biochemistry 137:15–19
    [Google Scholar]
  5. Collins A. R., Knobler R. L., Powell H., Buchmeier M. J. 1982; Monoclonal antibodies to murine hepatitis virus 4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology 119:358–371
    [Google Scholar]
  6. Haspel M. V., Lampert P. W., Oldstone M. B. A. 1978; Temperature-sensitive mutants of mouse hepatitis virus produce a high incidence of demyelination. Proceedings of the National Academy of SciencesU.S.A 754033–1036
    [Google Scholar]
  7. Hilton A., Mizzen L., Macintyre G., Cheley S., Anderson R. 1986; Translational control in murine hepatitis virus infection. Journal of General Virology 67:923–932
    [Google Scholar]
  8. Hirano N., Gotto N., Makino S., Fujiwara K. 1981; Persistent infection with mouse hepatitis virus JHM strain in DBT cell culture. Advances in Experimental Medicine and Biology 142:301–308
    [Google Scholar]
  9. Holmes K. V., Behnke J. N. 1981; Evolution of a coronavirus during persistent infection in vitro . Advances in Experimental Medicine and Biology 142:133–142
    [Google Scholar]
  10. Jackson D., Percy D., Morris V. L. 1984; Characterization of murine hepatitis virus (JHM) RNA from rats with experimental encephalomyelitis. Virology 137:297–304
    [Google Scholar]
  11. Lucas A., Flintoff W., Anderson R., Percy D., Coulter M., Dales S. 1977; In vivo and in vitro models of demyelinating diseases: tropism of the JHM strain of murine hepatitis virus for cells of glial origin. Cell 12:553–560
    [Google Scholar]
  12. Lucas A., Coulter M., Anderson R., Dales S., Flintoff W. 1978; In vivo and in vitro models of demyelinating diseases. II. Persistence and host-regulated thermosensitivity in cells of neural derivation infected with mouse hepatitis and measles viruses. Virology 88:325–337
    [Google Scholar]
  13. Mizzen L., Cheley S., Rao M., Wolf R., Anderson R. 1983; Fusion resistance and decreased infectibility as major host cell determinants of coronavirus persistence. Virology 128:407–417
    [Google Scholar]
  14. Mizzen L., Macintyre G., Wong F., Anderson R. 1987; Translational regulation in mouse hepatitis virus infection is not mediated by altered intracellular ion concentrations. Journal of General Virology 68:2143–2151
    [Google Scholar]
  15. Nagashima K., Wege H., Meyermann R., Ter Meulen V. 1979; Demyelinating encephalomyelitis induced by long-term coronavirus infection in rats. A preliminary report. Acta neuropathologica 45:205–213
    [Google Scholar]
  16. Sorensen O., Percy D., Dales S. 1980; In vivo and in vitro models of demyelinating disease. III. JHM virus infection of rats. Archives of Neurology 37:478–484
    [Google Scholar]
  17. Sorensen O., Beushausen S., Puchalski S., Cheley S., Anderson R., Coulter-Mackie M., Dales S. 1984; In vivo and in vitro models of demyelinating diseases-VIII: genetic, immunologic and cellular influences on JHM virus infection of rats. Advances in Experimental Medicine and Biology 173:279–298
    [Google Scholar]
  18. Stohlman S. A., Sakaguchi A. Y., Weiner L. P. 1979; Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion. Virology 98:448–455
    [Google Scholar]
  19. Taguchi F., Siddell S., Wege H., Massa P., Ter Meulen V. 1987; Characterization of JHMV variants isolated from rat brain and cultured neural cells after wild type JHMV infection. Advances in Experimental Medicine and Biology 218:343–349
    [Google Scholar]
  20. Weiner L. P., Johnson R. T., Herndon R. M. 1973; Viral infections and demyelinating diseases. New England Journal of Medicine 228:1103–1110
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-763
Loading
/content/journal/jgv/10.1099/0022-1317-70-3-763
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error