1887

Abstract

Summary

The virulence of a deletion variant of herpes simplex virus type 2 (HSV-2) strain HG52 has been determined by intracranial inoculation of 3-week-old BALB/c mice. The variant JH2604 has a 1.5 kb deletion within each copy of the long repeat region (R) of the genome between 0 to 0.02 and 0.81 to 0.83 map units. JH2604 is avirulent for mice compared to the parental wild-type virus, and fails to replicate in mouse brain . Correction of the deletion by marker rescue resulted in the isolation of recombinants which gave LD values comparable to those of individual plaque stocks of the parental HG52. Introduction of the deletion into wild-type virus resulted in recombinants which on intracranial inoculation of mice were avirulent. The results imply that sequences within the 3 kb terminal portion of R are required for virulence of HSV-2 strain HG52.

Keyword(s): HSV-2 , recombinants and RL
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-705
1989-03-01
2019-10-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-705
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error