1887

Journal of General Virology header logo

Volume 70, Issue 3

A Human Cytomegalovirus-neutralizing Monoclonal Antibody Recognizes a Normal Cell Protein Free

Abstract

SUMMARY

A monoclonal antibody (MAb), N2, neutralized human cytomegalovirus (HCMV) infectivity in the absence of complement and recognized a normal cell protein. By immunofluorescence, MAb N2 detected antigens in uninfected human cells, but not in cells of non-human origin. Antigens were present at the membrane and were dispersed diffusely within the cytoplasm. MAb N2 immunoprecipitated a glycoprotein with an of 94K from uninfected and infected cells. In infected cells only, it also recognized a protein of 34K which was not linked to the 94K glycoprotein by disulphide bonds. N2 neutralized both laboratory and field isolates of HCMV. A study of the distribution of the N2 neutralization epitope recognized among fresh isolates of HCMV showed that only 67% of the isolates could be neutralized by this antibody. There was no correlation between the number of passages and the level of neutralization. The 34K polypeptide was present in cells infected by all isolates. It thus appears that, during virus assembly, HCMV acquires a normal cell protein that bears a neutralization epitope.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CMV , human , MAb and neutralization
©Society for General Microbiology 1989
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-673
1989-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/3/JV0700030673.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-3-673&mimeType=html&fmt=ahah

References

  1. Amadéi C., Tardy-Panit M., Couillin P., Coulon M., Cabau N., Boue A., Michelson S. 1983; Kinetic Study of the development and localization of human cytomegalovirus (HCMV)-induced antigens using monoclonal antibodies. Annales de Virologie 134E:165–180
     [Google Scholar]
  2. Andersen P., Andersen K. K. 1975; Smooth muscle antibodies and other tissue antibodies in cytomegalovirus infection. Clinical and Experimental Immunology 22:22–29
     [Google Scholar]
  3. Beck S., Barrell B. G. 1987; Human cytomegalovirus encodes a glycoprotein homologous to MHC class I antigens. Nature, London 331:269–272
     [Google Scholar]
  4. Betts R. F. 1984; The relationship of epidemiology and treatment factors to infection and allograft survival in renal transplantation. In CMV: Pathogenesis and Prevention of Human Infection87–99 Birth Defects Original Article Series Plotkin S. A., Michelson S., Pagano J., Rapp F. New York: Alan R. Liss;
     [Google Scholar]
  5. Borysiewicz L. K., Rodgers B., Morris S., Graham S., Sissons J. G. P. 1985; Lysis of human cytomegalovirus infected fibroblasts by natural killer cells: demonstration of an interferon independent component requiring expression of early viral proteins and characterization of effector cells. Journal of Immunology 134:2695–2701
     [Google Scholar]
  6. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
     [Google Scholar]
  7. Britt W. J. 1984; Neutralizing antibodies detect a disulfide-linked glycoprotein complex within the envelope of human cytomegalovirus. Virology 135:369–378
     [Google Scholar]
  8. Chanarin I., Walford D. 1973; Thrombocytopenic purpura in cytomegalovirus mononucleosis. Lancet ii:238–239
     [Google Scholar]
  9. Colimon R., Somogyi T., Bertrand C., Michelson S. 1985; Demonstration of the colinearity of human cytomegalovirus genomes and construction of restriction maps of unknown isolates using cloned subgenomic fragments. Journal of General Virology 66:2183–2198
     [Google Scholar]
  10. Crawford L., Leppard K., Lane D., harlow E. 1982; Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen. Journal of Virology 42:612–620
     [Google Scholar]
  11. Dales S., Fujinami R. S., Oldstone M. B. A. 1983; Infection with vaccinia favors the selection of hybridomas synthesizing autoantibodies against intermediate filaments, one of them cross-reacting with the virus hemagglutinin. Journal of Immunology 131:1546–1553
     [Google Scholar]
  12. Farrar G. H., Greenaway P. J. 1986; Characterization of glycoprotein complexes present in human cytomegalovirus envelopes. Journal of General Virology 67:1469–1473
     [Google Scholar]
  13. Fiala M., Kattlove H., Lemikin S. 1973; Cytomegalovirus mononucleosis and severe thrombocytopenia. Annals of Internal Medicine 79:450–451
     [Google Scholar]
  14. Fujinami R. S., Oldstone M. B. A., Wroblewska Z., Frankel M. E., Koprowski H. 1983; Molecular mimicry in virus infection: cross reaction of measles virus phosphoprotein or of herpes simplex virus protein with human intermediate filaments. Proceedings of the National Academy of SciencesU.S.A 802346–2350
     [Google Scholar]
  15. Fujinami R. S., Nelson J. A., Walker L., Oldstone M. B. A. 1987; Sequence homology and immunologic cross-reactivity of human cytomegalovirus with HLA-DR β chain: a means for graft rejection and immunosuppression. Journal of Virology 62:100–105
     [Google Scholar]
  16. Gibson W. 1983; Protein counterparts of human and simian cytomegaloviruses. Virology 128:391–406
     [Google Scholar]
  17. Gould E. A., Chanas A. C., Buckley A., Clegg C. S. 1983; Monoclonal immunoglobulin M antibody to Japanese encephalitis virus that can react with a nuclear antigen in mammalian cells. Infection and Immunity 41:774–779
     [Google Scholar]
  18. Gupta P., St. Jeor S., Rapp F. 1977; Comparison of the polypeptides of several strains of human cytomegalovirus. Journal of General Virology 34:447–454
     [Google Scholar]
  19. Harris A. I., Meyers J. D. 1975; Cytomegalovirus-induced thrombocytopenia and hemolysis in an adult. Annals of Internal Medicine 83:670–671
     [Google Scholar]
  20. Haynes B. F., Hemler M., Cotner T., Mann D. L., Eisenbarth G. S., Strominger J. L., Fauci S. 1981; Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. Journal of Immunology 127:347–351
     [Google Scholar]
  21. Irmiere A., Gibson W. 1983; Isolation and characterization of a non-infectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118–130
     [Google Scholar]
  22. Irmiere A., Gibson W. 1985; Isolation of human cytomegalovirus intranuclear capsids, characterization of their protein constituents and demonstration that the B-capsid assembly protein is also abundant in non-infectious enveloped particles. Virology 130:277–283
     [Google Scholar]
  23. Jacobs J. P., Tones C. M., Baille J. P. 1970; Characteristics of a human diploid cell designated MRC-5. Nature, London 227:168–170
     [Google Scholar]
  24. Kari B., Lussenhop N., Goertz R., Wabuke-Bunoti M., Radeke R., Gehrz R. 1986; Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. Journal of Virology 60:345–352
     [Google Scholar]
  25. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
     [Google Scholar]
  26. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature, London 227:680–685
     [Google Scholar]
  27. Landini M. P., Severi B., Badiali L., Gonczol E., Mirolo O. 1987; Structural component of human cytomegalovirus: in situ localization of the major glycoprotein. Intervirology 27:154–161
     [Google Scholar]
  28. Lane D. P., Hoeffler W. K. 1980; SV40 large T shares an antigenic-determinant with a cellular protein of molecular weight 68,000. Nature, London 288:167–170
     [Google Scholar]
  29. Lönnqvist B., Ringden O., Wahren B., Gahrton G., Lundgren G. 1984; Cytomegalovirus infection associated with and preceding chronic graft-versus-host disease. Transplantation 38:465–468
     [Google Scholar]
  30. Mar E.-C., Patel P. C., Huang E.-S. 1981; Human cytomegalovirus-associated DNA polymerase and protein kinase activities. Journal of General Virology 57:149–156
     [Google Scholar]
  31. Mazeron M. C., Berba D., Guillemin M. C., Colimon R., Rossito A., Pérol Y. 1983; Production of monoclonal antibodies against human cytomegalovirus. Compte rendu hebdomadaire des séances de I’Académie des sciences, section III 297:305–308
     [Google Scholar]
  32. Revello M. G., Percivalle E., Gerna G. 1986; Immunoglobulin M to the membrane of uninfected fibroblasts in primary human cytomegalovirus infections. Microbiologica 9:127–138
     [Google Scholar]
  33. Rüger R., Bornkamm G. W., Fleckenstein B. 1984; Human cytomegalovirus DNA sequences with homologies to the cellular genome. Journal of General Virology 65:1351–1364
     [Google Scholar]
  34. Sarov I., Abady I. 1977; Human Cytomegalovirions And dense bodies: glycopeptide analysis and mechanism of cell rounding and polykaryocytosis. Israel Journal of Medical Sciences 13:887–895
     [Google Scholar]
  35. Schmidt H., Muller-Lantzch N., Peteler G. 1980; Human immune response to proteins of cytomegalovirus. Intervirology 13:154–161
     [Google Scholar]
  36. Severi B., Landini M. P., Govoni E. 1988; Human cytomegalovirus morphogenesis: an ultrastructural study of late cytoplasmic phases. Archives of Virology 98:51–64
     [Google Scholar]
  37. Shaw S. B., Rasmussen R. D., Mcdonough S. H., Staprans S. I., Vacquier J. P., Spector D. h. 1985; Cell-related sequences in the DNA genome of human cytomegalovirus strain Ad-169. Journal of Virology 55:843–848
     [Google Scholar]
  38. Sheshberadaran H., Norrby E. 1984; Three monoclonal antibodies against measles virus F protein cross-react with cellular stress proteins. Journal of Virology 52:995–999
     [Google Scholar]
  39. Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. 1985; Monoclonal antibodies for rapid laboratory detection of cytomegalovirus infection: characterization and diagnostic applications. Mayo Clinic Proceedings 60577–585
     [Google Scholar]
  40. Smith J. D., De Harven E. 1973; Herpes simplex and human cytomegalovirus replication in WI-38 cells. I. Sequence of viral replication. Journal of Virology 12:919–930
     [Google Scholar]
  41. Stagno S., Pass R. F., Dworsky M. E., Britt W. J., Alford C. A. 1984; Congenital and perinatal cytomegalovirus infections: clinical characteristics and pathogenic factors. In CMV: Pathogenesis and Prevention of Human Infection65–85 Birth Defects Original Article Series Plotkin S. A., Michelson S., Pagano J., Rapp F. New York: Alan R. Liss;
     [Google Scholar]
  42. Stinski M. F. 1976; Human cytomegalovirus: glycoproteins associated with virions and dense bodies. Journal of Virology 56:665–675
     [Google Scholar]
  43. Staprans S. I., Spector D. H. 1986; 2·2-kilobase class of early transcripts encoded by cell-related sequences in human cytomegalovirus strain Ad-169. Journal of Virology 57:591–602
     [Google Scholar]
  44. Tardieu M., Powers M. L., Hafler D. A., Hauser S. L., Weiner H. L. 1984; Autoimmunity following viral infection: demonstration of monoclonal antibodies against normal tissue following infection of mice with reovirus and demonstration of shared antigenicity between virus and lymphocytes. European Journal of Immunology 14:561–565
     [Google Scholar]
  45. Trowbridge I. S., Omary M. B. 1981; Human cell surface glycoprotein related to cell proliferation is the receptor for transferrin. Proceedings of the National Academy of SciencesU.S.A 791175–1179
     [Google Scholar]
  46. Wager O., Rasanen J. A., Hagman A., Klemola E. 1968; Mixed cryoimmunoglobulinemia in infectious mononucleosis and cytomegalovirus mononucleosis. International Archives of Allergy and Applied Immunology 35:345–361
     [Google Scholar]
  47. Weiner D., Gibson W. 1981; Identification of a primate cytomegalovirus group-common protein antigen. Virology 115:182–191
     [Google Scholar]
  48. Wiley D. 1987; MHC gene in cytomegalovirus. Nature, London 331:209–210
     [Google Scholar]
  49. Wray W., Boulikas T., Wray V. P., Hannock R. 1981; Silver staining of proteins in polyacrylamide gels. Analytical Biochemistry 118:197–202
     [Google Scholar]
  50. Zeuler W. W., Stulberg C. S., Page R. H., Teruya J., Brough A. J. 1966; Etiology and pathogenesis of acquired hemolytic anemia. Transfusion 6:438–461
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-673
Loading
A Human Cytomegalovirus-neutralizing Monoclonal Antibody Recognizes a Normal Cell Protein
J. Gen. Virol. 70, 673 (1989); https://doi.org/10.1099/0022-1317-70-3-673
/content/journal/jgv/10.1099/0022-1317-70-3-673
/content/journal/jgv/10.1099/0022-1317-70-3-673
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error